Scanning Electrochemical Microscopy for Imaging Single Cells and Biomolecules
This article presents an overview of the recent progress in scanning electrochemical microscopy (SECM) for imaging single cells and biomolecules. SECM is a technique for characterizing the local electrochemical nature of various materials by scanning a probe microelectrode. The probe current reflects the electrochemical processes occurring in the small space surrounded by the probe and the substrate. The spatial resolution of SECM is inferior to conventional scanning probe microscopes such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM), since the fabrication of the probe microelectrodes with nanometer sizes is quite difficult. However, recent progress in the fabrication of nanometer-scale electrodes and the development of electrode–sample distance control systems has greatly enhanced the capacity of SECM systems to solve problems in cell biology. The topics reviewed include the following: enzyme activity evaluation, electrochemical enzyme-linked immunosorbent assays, membrane permeability evaluation, respiratory activity measurements, reporter gene assays, membrane protein imaging, and neurotransmitter detection.
KeywordsEpidermal Growth Factor Receptor Bilayer Lipid Membrane Redox Species Scan Electrochemical Microscopy Epidermal Growth Factor Receptor Expression Level
- Kaya T, Nagamine K, Matsui N, Yasukawa T, Shiku H, Matsue T (2004) On-chip electrochemical measurement of beta-galactosidase expression using a microbial chip. Chem Commun (2):248–249Google Scholar
- Novak P, Li C, Shevchuk AI, Stepanyan R, Caldwell M, Hughes S, Smart TG, Gorelik J, Ostanin VP, Lab MJ, Moss GWJ, Frolenkov GI, Klenerman D, Korchev YE (2009) Nanoscale live-cell imaging using hopping probe ion conductance microscopy. Nat Methods 6(4):279–281CrossRefPubMedCentralPubMedGoogle Scholar
- Takahashi Y, Shevchuk AI, Novak P, Zhang YJ, Ebejer N, Macpherson JV, Unwin PR, Pollard AJ, Roy D, Clifford CA, Shiku H, Matsue T, Klenerman D, Korchev YE (2011b) Multifunctional nanoprobes for nanoscale chemical imaging and localized chemical delivery at surfaces and interfaces. Angew Chem Int Ed 50(41):9638–9642CrossRefGoogle Scholar
- Takahashi Y, Shevchuk AI, Novak P, Babakinejad B, Macpherson J, Unwin PR, Shiku H, Gorelik J, Klenerman D, Korchev YE, Matsue T (2012) Topographical and electrochemical nanoscale imaging of living cells using voltage-switching mode scanning electrochemical microscopy. Proc Natl Acad Sci U S A 109(29):11540–11545CrossRefPubMedCentralPubMedGoogle Scholar