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Nanobiosensors and Nanobioanalyses pp 197–249Cite as

Semiconductor Quantum Dots and Energy Transfer for Optical Sensing and Bioanalysis: Applications

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Abstract

Semiconductor quantum dots (QDs) are very promising materials for optical sensing and bioanalysis. This chapter builds on Chap. 10, which reviewed the optical properties of QDs and their benefits for energy transfer, by illustrating the utility of QDs and energy transfer for optical sensing and bioanalysis. Representative examples of different in vitro assays and cellular probes from the literature are described. Energy transfer mechanisms including Förster resonance energy transfer (FRET ), bioluminescence and chemiluminescence resonance energy transfer (BRET and CRET ), nanosurface energy transfer (NSET ), and charge transfer can be used for optical signal generation in homogeneous assays, single-particle assays, and heterogeneous assays targeting bioanalytes as diverse as nucleic acids, proteins, small molecules, ions, and the activity of enzymes such as proteases, kinases, and nucleases. The importance and versatility of QDs in optical sensing and bioanalysis has been growing steadily since their introduction and will continue to grow in the near future as QD-based assays are optimized and applied to new problems, and new capabilities are developed.

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  1. Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC, V6T 1Z1, Canada

    Miao Wu & W. Russ Algar

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  1. Miao Wu
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  1. School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan

    Mun'delanji C. Vestergaard

  2. Department of Physical and Environmental Sciences, University of Toronto Scarborough, Toronto, Ontario, Canada

    Kagan Kerman

  3. Department of Chemical and Biomolecular Engineering, The Hong Kong University of Science and Technology, Kowloon, Hong Kong SAR

    I-Ming Hsing

  4. Graduate School of Engineering, Osaka University, Osaka, Japan

    Eiichi Tamiya

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© 2015 Springer Japan

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Wu, M., Algar, W.R. (2015). Semiconductor Quantum Dots and Energy Transfer for Optical Sensing and Bioanalysis: Applications. In: Vestergaard, M., Kerman, K., Hsing, IM., Tamiya, E. (eds) Nanobiosensors and Nanobioanalyses. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55190-4_11

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