Abstract
Vascular endothelial cells are constantly exposed to shear stress generated by blood flow and respond by exhibiting alterations in morphology and cytoskeletal structures as well as by modulating cell physiological functions. Since endothelial cell responses to fluid shear stress have been implicated in the localization of atherosclerosis, the effects of fluid shear stress on endothelial cell morphology and functions have been exclusively studied. Interestingly, atherosclerosis occurs primarily at branching and curving regions of arterial walls, where endothelial cells would experience complex blood flow patterns. So far, a lot of efforts have been made to study endothelial mechanotransduction to flow, indicating the fact that after applying fluid shear stress, endothelial cells exhibit marked elongation and orientation in the direction of flow. The need for experimental techniques for studying endothelial cell responses to flow has lead to the development of different types of flow chambers. Conventional flow chambers include a cone-and-plate flow chamber and a parallel-plate flow chamber, while more recently, microfluidic flow chambers have emerged with a great potential for a high throughput analysis. In this chapter, many types of flow chambers are first summarized. Stiffness change of sheared endothelial cells has been be of great interest in particular for mechanical engineering researchers because endothelial cells may change their morphology and cytoskeletal structures in response to fluid shear stress. Therefore, AFM (Atomic Force Microscopy) stiffness measurement of sheared endothelial cells is then described. Lastly, stiffness change of sheared endothelial cell nuclei measured with a pipette aspiration test is also presented.
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References
Barbee KA, Mundel T, Lal R, Davies PF (1995) Subcellular distribution of shear stress at the surface of flow-aligned and nonaligned endothelial monolayers. Am J Physiol 268:H1765–H1772
Caille N, Tardy Y, Meister J-J (1998) Assessment of strain field in endothelial cells subjected to uniaxial deformation of their substrate. Ann Biomed Eng 26:409–416
Caille N, Thoumine O, Tardy Y, Meister J-J (2002) Contribution of the nucleus to the mechanical properties of endothelial cells. J Biomech 35:177–187
Chau L, Doran M, Cooper-White J (2009) A novel multishear microdevice for studying cell mechanics. Lab Chip 9:1897–1902
Chen J, Fabry B, Schiffrin EL, Wang N (2001) Twisting integrin receptors increases endothelin-1 gene expression in endothelial cells. Am J Physiol Cell Physiol 280:C1475–C1484
Chien S (2007) Mechanotransduction and endothelial cell homeostasis: the wisdom of the cell. Am J Physiol Heart Circ Physiol 292:H1209–H1224
Davies PF (1995) Flow-mediated endothelial mechanotransduction. Physiol Rev 75:519–560
Deguchi S, Maeda K, Ohashi T, Sato M (2005) Flow-induced hardening of endothelial nucleus as an intracellular stress-bearing organelle. J Biomech 38:1751–1759
Dewey CF, DePaola N (1989) Exploring flow-cell interactions using computatioal fluid dynamics. In: Woo SL-Y, Seguchi Y (eds) Tissue eng. ASME, New York, pp 31–33
Dewey CF Jr, Bussolari SR, Gimbrone MA Jr, Davies PF (1981) The dynamic response of vascular endothelial cells to fluid shear stress. J Biomech Eng 103:177–185
Flaherty JT, Pierce JE, Ferrans VJ, Patel DJ, Tucker WK, Fry DL (1972) Endothelial nuclear patterns in the canine arterial tree with p reference to hemodynamic events. Circ Res 30:23–33
Fukushima S, Ngatsu A, Kaibara M, Oka K, Tanishita K (2001) Measurement of surface topography of endothelial cell and wall shear stress distribution on the cell. JSME Int J Ser C 44:972–980
Garin G, Berk BC (2006) Flow-mediated signaling modulates endothelial cell phenotype. Endothelium 13:375–384
Guilak F (1995) Compression-induced changes in the shape and volume of the chondrocyte nucleus. J Biomech 28:1529–1541
Hazel AL, Pedley TJ (2000) Vascular endothelial cells minimize the total force on their nuclei. Biophys J 78:47–54
Helmke BP, Davies PF (2002) The cytoskeleton under external fluid mechanical forces: hemodynamic forces acting on the endothelium. Ann Biomed Eng 30:284–296
Hsu S, Thakar R, Liepmann D, Li S (2005) Effects of shear stress on endothelial cell haptotaxis on micropatterned surfaces. Biochem Biophys Res Commun 337:401–409
Ingber DE (1990) Fibronectin controls capillary endothelial cell growth by modulating cell shape. Proc Natl Acad Sci U S A 87:3579–3583
Kataoka N, Ujita S, Sato M (1998) Effect of flow direction on the morphological responses of cultured bovine aortic endothelial cells. Med Biol Eng Comput 36:122–128
Levesque MJ, Nerem RM (1985) The elongation and orientation of cultured endothelial cells in response to shear stress. Trans ASME J Biomech Eng 107:341–347
Maniotis AJ, Chen CS, Ingber DE (1997) Demonstration of mechanical connections between integrins, cytoskeletal filaments, and nucleoplasm that stabilize nuclear structure. Proc Natl Acad Sci U S A 94:849–854
Mott RE, Helmke BP (2007) Mapping the dynamics of shear stress-induced structural changes in endothelial cells. Am J Physiol Cell Physiol 293:C1616–C1626
Nerem RM, Levesque MJ, Cornhill JF (1981) Vascular endothelial morphology as an indicator of the pattern of blood flow. Trans ASME J Biomech Eng 103:172–176
Ogunrinade O, Kameya GT, Truskey GA (2002) Effect of fluid shear stress on the permeability of the arterial endothelium. Ann Biomed Eng 30:430–446
Ohashi T, Sato M (2005) Remodeling of vascular endothelial cells exposed to fluid shear stress: experimental and numerical approach. Fluid Dyn Res 37:40–59
Ohashi T, Sato M (2012) Chapter 22, Endothelial cell responses to fluid shear stress: from methodology to applications. In: Dias R, Martins AA, Lima R, Mata TM (eds) Single and two-phase flows on chemical and biomedical engineering. Bentham Science Publishers, Oak Park, pp 372–385
Ohashi T, Sugawara H, Matsumoto T, Sato M (2000) Surface topography measurement and intracellular stress analysis of cultured endothelial cells exposed to fluid shear stress. JSME Int J Ser C 43:780–786
Ohashi T, Ishii Y, Ishikawa Y, Matsumoto T, Sato M (2002) Experimental and numerical analyses of local mechanical properties measured by atomic force microscopy for sheared endothelial cells. Bio-Med Mater Eng 12:319–327
Ohashi T, Hanamura K, Azuma D, Sakamoto N, Sato M (2008) Remodeling of endothelial cell nucleus exposed to three different mechanical stimuli. J Biomech Sci Eng 3(2):63–74
Radmacher M, Fritz M, Hansma PK (1995) Imaging soft samples with the atomic force microscope: gelatin in water and propanol. Biophys J 69:264–270
Satcher RL Jr, Bussolari SR, Gimbrone MA Jr, Dewey CF Jr (1992) The distribution of fluid forces on model arterial endothelium using computational fluid dynamics. Trans ASME J Biomech Eng 114:309–316
Sato M, Levesque MJ, Nerem RM (1987) Micropipette aspiration of cultured bovine aortic endothelial cells exposed to shear stress. Arterioscler Thromb Vasc Biol 7:276–286
Sato M, Nagayama K, Kataoka N, Sasaki M, Hane K (2000) Local mechanical properties measured by atomic force microscopy for cultured endothelial cells exposed to shear stress. J Biomech 33:127–135
Shin M, Matsuda K, Ishii O et al (2004) Endothelialized networks with a vascular geometry in microfabricated poly (dimethyl siloxane). Biomed Microdevices 6:269–278
Theret DP, Levesque MJ, Sato M, Nerem RM, Wheeler LT (1988) The application of a homogeneous half-space model in the analysis of endothelial cell micropipette measurements. Trans ASME J Biomech Eng 110:190–199
Tsou JK, Gower RM, Ting HJ et al (2008) Spatial regulation of inflammation by human aortic endothelial cells in a linear gradient of shear stress. Microcirculation 15:311–323
White CR, Haidekker M, Bao X, Frangos JA (2001) Temporal gradients in shear, but not spatial gradients, stimulate endothelial cell proliferation. Circulation 103:2508–2513
Yamaguchi T, Yamamoto Y, Liu H (2000) Computational mechanical model studies on the spontaneous emergent morphogenesis of the cultured endothelial cells. J Biomech 33:115–126
Young EWK, Simmons CA (2010) Macro-and microscale fluid flow systems for endothelial cell biology. Lab Chip 10:143–160
Young EWK, Wheeler AR, Simmons CA (2007) Matrix-dependent adhesion of vascular and valvular endothelial cells in microfluidic channels. Lab Chip 7:1759–1766
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Ohashi, T. (2016). Mechanical Characterization of Vascular Endothelial Cells Exposed to Fluid Shear Stress. In: Tanishita, K., Yamamoto, K. (eds) Vascular Engineering. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54801-0_6
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DOI: https://doi.org/10.1007/978-4-431-54801-0_6
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