Induction of Human Pluripotent Stem Cells by the Sendai Virus Vector: Establishment of a Highly Efficient and Footprint-Free System
The Sendai virus (SeV) vector system exhibited very high performance in transgene expression and a broad target tissue/cell range. Together with its nonintegrating nature, the potential of this vector has been suggested to be an efficient and valuable tool for generating induced pluripotent stem cells (iPSCs). Initial stage studies have shown the potential of the SeV vector and demonstrated that this system is simple and generates vector/transgene-free iPSCs with high efficiency. Subsequently, a more controllable method to eliminate the vector/transgenes from generated iPSCs has been developed by introducing several temperature-sensitive mutations into the SeV vector backbone. Based on this method, human iPSCs have been established from cord blood CD34+ cells, T cells from peripheral blood, and samples of patients with intractable diseases. In addition, a model of regenerative medicine with gene correction was presented using SeV vector-derived iPSCs from a patient with an inherited disease.
KeywordsGreen Fluorescent Protein Vector Backbone Cord Blood Cell iPSC Line Human iPSCs
We are grateful to M. Hasegawa and A. Iida at DNAVEC Corporation for critical reading of this manuscript. We thank Y. Yonemitsu at Kyushu University for his helpful advice.
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