Reverse Genetics of Mononegavirales: The Rabies Virus Paradigm

  • Karl-Klaus Conzelmann


The neurotropic rabies virus (RABV) is a prototype member of the Mononegavirales order of viruses and is the most significant human pathogen of the Rhabdoviridae family. A reverse genetics system for RABV was established almost 20 years ago, providing a paradigm for other Mononegavirales members as well. The availability of engineered recombinant viruses opened a new era to study common aspects of Mononegavirales biology and specific aspects of the unique lifestyle and pathogenesis of individual members. Above all, the knowledge gained has allowed engineering of beneficial biomedical tools such as viral vectors, vaccines, and tracers. In this chapter, the development of the classical rabies virus reverse genetics approach is described, and some of the most exciting biomedical applications for recombinant RABV and other Mononegavirales are briefly addressed.


Newcastle Disease Virus Rabies Virus Vesicular Stomatitis Virus Reverse Genetic Hepatitis Delta Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Research in the author’s laboratory is funded by the German Research Foundation (DFG) through SFB 870 and Grako 1202, and the German Federal Ministry of Education and Research (BMBF) grant no. 01KI1016B. I thank Kerstin Schuhmann and Yoshiyuki Nagai for valuable comments on the manuscript.


  1. Ammayappan A, Lapatra SE, Vakharia VN (2010) A vaccinia-virus-free reverse genetics system for infectious hematopoietic necrosis virus. J Virol Methods 167:132–139PubMedGoogle Scholar
  2. Astic L, Saucier D, Coulon P, Lafay F, Flamand A (1993) The CVS strain of rabies virus as transneuronal tracer in the olfactory system of mice. Brain Res 619:146–156PubMedGoogle Scholar
  3. Baltimore D (1971) Expression of animal virus genomes. Bacteriol Rev 35:235–241PubMedCentralPubMedGoogle Scholar
  4. Baltimore D, Huang AS, Stampfer M (1970) Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci USA 66:572–576PubMedGoogle Scholar
  5. Baron MD, Barrett T (1997) Rescue of rinderpest virus from cloned cDNA. J Virol 71:1265–1271PubMedCentralPubMedGoogle Scholar
  6. Biacchesi S (2011) The reverse genetics applied to fish RNA viruses. Vet Res 42:12PubMedGoogle Scholar
  7. Blount KF, Uhlenbeck OC (2002) The hammerhead ribozyme. Biochem Soc Trans 30:1119–1122PubMedGoogle Scholar
  8. Boonyaratanakornkit J, Bartlett E, Schomacker H, Surman S, Akira S, Bae YS, Collins P, Murphy B, Schmidt A (2011) The C proteins of human parainfluenza virus type 1 limit double-stranded RNA accumulation that would otherwise trigger activation of MDA5 and protein kinase R. J Virol 85:1495–1506PubMedCentralPubMedGoogle Scholar
  9. Brandler S, Ruffie C, Najburg V, Frenkiel MP, Bedouelle H, Despres P, Tangy F (2010) Pediatric measles vaccine expressing a dengue tetravalent antigen elicits neutralizing antibodies against all four dengue viruses. Vaccine 28:6730–6739PubMedGoogle Scholar
  10. Bridgen A, Elliott RM (1996) Rescue of a segmented negative-strand RNA virus entirely from cloned complementary DNAs. Proc Natl Acad Sci USA 93:15400–15404PubMedGoogle Scholar
  11. Bruns AM, Horvath CM (2012) Activation of RIG-I-like receptor signal transduction. Crit Rev Biochem Mol Biol 47:194–206PubMedCentralPubMedGoogle Scholar
  12. Brzózka K, Finke S, Conzelmann KK (2005) Identification of the rabies virus alpha/beta interferon antagonist: phosphoprotein P interferes with phosphorylation of interferon regulatory factor 3. J Virol 79:7673–7681PubMedCentralPubMedGoogle Scholar
  13. Brzózka K, Finke S, Conzelmann KK (2006) Inhibition of interferon signaling by rabies virus phosphoprotein P: activation-dependent binding of STAT1 and STAT2. J Virol 80:2675–2683Google Scholar
  14. Buchholz UJ, Finke S, Conzelmann KK (1999) Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. J Virol 73:251–259PubMedCentralPubMedGoogle Scholar
  15. Calain P, Roux L (1993) The rule of six, a basic feature for efficient replication of Sendai virus defective interfering RNA. J Virol 67:4822–4830PubMedCentralPubMedGoogle Scholar
  16. Cattaneo R, Miest T, Shashkova EV, Barry MA (2008) Reprogrammed viruses as cancer therapeutics: targeted, armed and shielded. Nat Rev Microbiol 6:529–540PubMedGoogle Scholar
  17. Collins PL, Hill MG, Camargo E, Grosfeld H, Chanock RM, Murphy BR (1995) Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5′ proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development. Proc Natl Acad Sci USA 92:11563–11567PubMedGoogle Scholar
  18. Conzelmann KK (1996) Genetic manipulation of non-segmented negative-strand RNA viruses. J Gen Virol 77(pt 3):381–389PubMedGoogle Scholar
  19. Conzelmann KK (2004) Reverse genetics of Mononegavirales. Curr Top Microbiol Immunol 283:1–41PubMedGoogle Scholar
  20. Conzelmann KK, Schnell M (1994) Rescue of synthetic genomic RNA analogs of rabies virus by plasmid-encoded proteins. J Virol 68:713–719PubMedCentralPubMedGoogle Scholar
  21. Cui S, Eisenacher K, Kirchhofer A, Brzózka K, Lammens A, Lammens K, Fujita T, Conzelmann KK, Krug A, Hopfner KP (2008) The C-terminal regulatory domain is the RNA 5′-triphosphate sensor of RIG-I. Mol Cell 29:169–179PubMedGoogle Scholar
  22. Dum RP, Strick PL (2012) Transneuronal tracing with neurotropic viruses reveals network macroarchitecture. Curr Opin Neurobiol doi: 10.1016/j.conb.2012.12.002
  23. Durbin AP, Hall SL, Siew JW, Whitehead SS, Collins PL, Murphy BR (1997) Recovery of infectious human parainfluenza virus type 3 from cDNA. Virology 235:323–332PubMedGoogle Scholar
  24. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE (2000) Spread and pathogenic characteristics of a G-deficient rabies virus recombinant: an in vitro and in vivo study. J Gen Virol 81:2147–2153PubMedGoogle Scholar
  25. Finke S, Conzelmann KK (1997) Ambisense gene expression from recombinant rabies virus: random packaging of positive- and negative-strand ribonucleoprotein complexes into rabies virions. J Virol 71:7281–7288PubMedCentralPubMedGoogle Scholar
  26. Finke S, Cox JH, Conzelmann KK (2000) Differential transcription attenuation of rabies virus genes by intergenic regions: generation of recombinant viruses overexpressing the polymerase gene. J Virol 74:7261–7269PubMedCentralPubMedGoogle Scholar
  27. Finke S, Mueller-Waldeck R, Conzelmann KK (2003) Rabies virus matrix protein regulates the balance of virus transcription and replication. J Gen Virol 84:1613–1621PubMedGoogle Scholar
  28. Fitzgerald KA, McWhirter SM, Faia KL, Rowe DC, Latz E, Golenbock DT, Coyle AJ, Liao SM, Maniatis T (2003) IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat Immunol 4:491–496PubMedGoogle Scholar
  29. Ford E, Thanos D (2010) The transcriptional code of human IFN-beta gene expression. Biochim Biophys Acta 1799:328–336PubMedGoogle Scholar
  30. Fu ZF (2005) Genetic comparison of the Rhabdoviruses from animals and plants. Curr Top Microbiol Immunol 292:1–24PubMedGoogle Scholar
  31. Fuerst TR, Niles EG, Studier FW, Moss B (1986) Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci USA 83:8122–8126PubMedGoogle Scholar
  32. Garcin D, Pelet T, Calain P, Roux L, Curran J, Kolakofsky D (1995) A highly recombinogenic system for the recovery of infectious Sendai paramyxovirus from cDNA: generation of a novel copy-back nondefective interfering virus. EMBO J 14:6087–6094PubMedGoogle Scholar
  33. Geisbert TW, Feldmann H (2011) Recombinant vesicular stomatitis virus-based vaccines against Ebola and Marburg virus infections. J Infect Dis 204(Suppl 3):S1075–S1081PubMedGoogle Scholar
  34. Gerlier D, Lyles DS (2011) Interplay between innate immunity and negative-strand RNA viruses: towards a rational model. Microbiol Mol Biol Rev 75:468–490PubMedCentralPubMedGoogle Scholar
  35. Ghanem A, Kern A, Conzelmann KK (2012) Significantly improved rescue of rabies virus from cDNA plasmids. Eur J Cell Biol 91:10–16PubMedGoogle Scholar
  36. Ginger M, Haberl M, Conzelmann KK, Schwarz MK, Frick A (2013) Revealing the secrets of neuronal circuits with recombinant rabies virus technology. Front Neural Circuits 7:2. doi: 10.3389/fncir.2013.00002, Epub@2013 Jan 24:2PubMedCentralPubMedGoogle Scholar
  37. Goff SP, Berg P (1976) Construction of hybrid viruses containing SV40 and lambda phage DNA segments and their propagation in cultured monkey cells. Cell 9:695–705PubMedGoogle Scholar
  38. Gomme EA, Wanjalla CN, Wirblich C, Schnell MJ (2011) Rabies virus as a research tool and viral vaccine vector. Adv Virus Res 79:139–164PubMedGoogle Scholar
  39. Goodbourn S, Randall RE (2009) The regulation of type I interferon production by paramyxoviruses. J Interferon Cytokine Res 29:539–547PubMedGoogle Scholar
  40. He B, Paterson RG, Ward CD, Lamb RA (1997) Recovery of infectious SV5 from cloned DNA and expression of a foreign gene. Virology 237:249–260PubMedGoogle Scholar
  41. Hoffman MA, Banerjee AK (1997) An infectious clone of human parainfluenza virus type 3. J Virol 71:4272–4277PubMedCentralPubMedGoogle Scholar
  42. Honda K, Takaoka A, Taniguchi T (2006) Type I interferon [corrected] gene induction by the interferon regulatory factor family of transcription factors. Immunity 25:349–360PubMedGoogle Scholar
  43. Hornung V, Ellegast J, Kim S, Brzózka K, Jung A, Kato H, Poeck H, Akira S, Conzelmann KK, Schlee M, Endres S, Hartmann G (2006) 5′-Triphosphate RNA is the ligand for RIG-I. Science 314:994–997PubMedGoogle Scholar
  44. Huang Y, Tang Q, Nadin-Davis SA, Zhang S, Hooper CD, Ming P, Du J, Tao X, Hu R, Liang G (2010) Development of a reverse genetics system for a human rabies virus vaccine strain employed in China. Virus Res 149:28–35PubMedGoogle Scholar
  45. Inoue K, Shoji Y, Kurane I, Iijima T, Sakai T, Morimoto K (2003) An improved method for recovering rabies virus from cloned cDNA. J Virol Methods 107:229–236PubMedGoogle Scholar
  46. Ito N, Moseley GW, Blondel D, Shimizu K, Rowe CL, Ito Y, Masatani T, Nakagawa K, Jans DA, Sugiyama M (2010) The role of interferon-antagonist activity of rabies virus phosphoprotein in viral pathogenicity. J Virol 84:6699–6710PubMedCentralPubMedGoogle Scholar
  47. Johnson JE, Schnell MJ, Buonocore L, Rose JK (1997) Specific targeting to CD4+ cells of recombinant vesicular stomatitis viruses encoding human immunodeficiency virus envelope proteins. J Virol 71:5060–5068PubMedCentralPubMedGoogle Scholar
  48. Kato A, Sakai Y, Shioda T, Kondo T, Nakanishi M, Nagai Y (1996) Initiation of Sendai virus multiplication from transfected cDNA or RNA with negative or positive sense. Genes Cells 1:569–579PubMedGoogle Scholar
  49. Kato H, Takahasi K, Fujita T (2011) RIG-I-like receptors: cytoplasmic sensors for non-self RNA. Immunol Rev 243:91–98PubMedGoogle Scholar
  50. Kawasaki T, Kawai T, Akira S (2011) Recognition of nucleic acids by pattern-recognition receptors and its relevance in autoimmunity. Immunol Rev 243:61–73PubMedGoogle Scholar
  51. Khattar SK, Samal S, Devico AL, Collins PL, Samal SK (2011) Newcastle disease virus expressing human immunodeficiency virus type 1 envelope glycoprotein induces strong mucosal and serum antibody responses in Guinea pigs. J Virol 85:10529–10541PubMedCentralPubMedGoogle Scholar
  52. Kimura Y (1973) Phenotypic mixing of vesicular stomatitis virus with HVJ (Sendai virus). Jpn J Microbiol 17:373–381PubMedGoogle Scholar
  53. Kinoh H, Inoue M, Komaru A, Ueda Y, Hasegawa M, Yonemitsu Y (2009) Generation of optimized and urokinase-targeted oncolytic Sendai virus vectors applicable for various human malignancies. Gene Ther 16:392–403PubMedGoogle Scholar
  54. Klingen Y, Conzelmann KK, Finke S (2008) Double-labeled rabies virus: live tracking of enveloped virus transport. J Virol 82:237–245PubMedCentralPubMedGoogle Scholar
  55. Lafon M (2011) Evasive strategies in rabies virus infection. Adv Virus Res 79:33–53PubMedGoogle Scholar
  56. Lamb RA (2007) Mononegavirales. In: Knipe DM, Howley PM (eds) Fields virology. Lippincott, Philadelphia, pp 1357–1362Google Scholar
  57. Lawson ND, Stillman EA, Whitt MA, Rose JK (1995) Recombinant vesicular stomatitis viruses from DNA. Proc Natl Acad Sci USA 92:4477–4481PubMedGoogle Scholar
  58. Le Mercier P, Garcin D, Hausmann S, Kolakofsky D (2002) Ambisense Sendai viruses are inherently unstable but are useful to study viral RNA synthesis. J Virol 76:5492–5502PubMedCentralPubMedGoogle Scholar
  59. Loo YM, Gale M Jr (2011) Immune signaling by RIG-I-like receptors. Immunity 34:680–692PubMedCentralPubMedGoogle Scholar
  60. Luytjes W, Krystal M, Enami M, Pavin JD, Palese P (1989) Amplification, expression, and packaging of foreign gene by influenza virus. Cell 59:1107–1113PubMedGoogle Scholar
  61. Marriott AC, Easton AJ (1999) Reverse genetics of the Paramyxoviridae. Adv Virus Res 53:321–340PubMedGoogle Scholar
  62. Marschalek A, Finke S, Schwemmle M, Mayer D, Heimrich B, Stitz L, Conzelmann KK (2009) Attenuation of rabies virus replication and virulence by picornavirus internal ribosome entry site elements. J Virol 83:1911–1919PubMedCentralPubMedGoogle Scholar
  63. Martin A, Staeheli P, Schneider U (2006) RNA polymerase II-controlled expression of antigenomic RNA enhances the rescue efficacies of two different members of the Mononegavirales independently of the site of viral genome replication. J Virol 80:5708–5715PubMedCentralPubMedGoogle Scholar
  64. Mebatsion T (2001) Extensive attenuation of rabies virus by simultaneously modifying the dynein light chain binding site in the P protein and replacing Arg333 in the G protein. J Virol 75:11496–11502PubMedCentralPubMedGoogle Scholar
  65. Mebatsion T, Conzelmann KK (1996) Specific infection of CD4+ target cells by recombinant rabies virus pseudotypes carrying the HIV-1 envelope spike protein. Proc Natl Acad Sci USA 93:11366–11370PubMedGoogle Scholar
  66. Mebatsion T, Konig M, Conzelmann KK (1996) Budding of rabies virus particles in the absence of the spike glycoprotein. Cell 84:941–951PubMedGoogle Scholar
  67. Mebatsion T, Finke S, Weiland F, Conzelmann KK (1997) A CXCR4/CD4 pseudotype rhabdovirus that selectively infects HIV-1 envelope protein-expressing cells. Cell 90:841–847PubMedGoogle Scholar
  68. Ming PG, Huang Y, Tang Q, Du JL, Tao XY, Yan JX, Hu RL (2009) Construction and analysis of full-length cDNA clone of rabies virus street strain. Bing Du Xue Bao 25:17–22PubMedGoogle Scholar
  69. Morodomi Y, Yano T, Kinoh H, Harada Y, Saito S, Kyuragi R, Yoshida K, Onimaru M, Shoji F, Yoshida T, Ito K, Shikada Y, Maruyama R, Hasegawa M, Maehara Y, Yonemitsu Y (2012) BioKnife, a uPA activity-dependent oncolytic Sendai virus, eliminates pleural spread of malignant mesothelioma via simultaneous stimulation of uPA expression. Mol Ther 20:769–777PubMedGoogle Scholar
  70. Motz C, Schuhmann KM, Kirchhofer A, Moldt M, Witte G, Conzelmann KK, Hopfner KP (2013) Paramyxovirus V proteins disrupt the fold of the RNA sensor MDA5 to inhibit antiviral signaling. Science 339:690–693PubMedGoogle Scholar
  71. Mourez T, Mesel-Lemoine M, Combredet C, Najburg V, Cayet N, Tangy F (2011) A chimeric measles virus with a lentiviral envelope replicates exclusively in CD4+/CCR5+ cells. Virology 419:117–125PubMedGoogle Scholar
  72. Muik A, Kneiske I, Werbizki M, Wilfingseder D, Giroglou T, Ebert O, Kraft A, Dietrich U, Zimmer G, Momma S, von Laer D (2011) Pseudotyping vesicular stomatitis virus witn lymphocytic choriomeningitis virus glycoproteins enhances infectivity for glioma cells and minimizes neurotropism. J Virol 85:5679–5684Google Scholar
  73. Nagai Y, Takakura A, Irie T, Yonemitsu Y, Gotoh B (2011) Sendai virus: evolution from mouse pathogen to a state-of-the-art tool in virus research and biotechnology. In: Samal SK (ed) The biology of paramyxoviruses. Caister Academic, Norfolk, pp 115–173Google Scholar
  74. Nishimura K, Sano M, Ohtaka M, Furuta B, Umemura Y, Nakajima Y, Ikehara Y, Kobayashi T, Segawa H, Takayasu S, Sato H, Motomura K, Uchida E, Kanayasu-Toyoda T, Asashima M, Nakauchi H, Yamaguchi T, Nakanishi M (2011) Development of defective and persistent Sendai virus vector: a unique gene delivery/expression system ideal for cell reprogramming. J Biol Chem 286:4760–4771PubMedGoogle Scholar
  75. Onoguchi K, Yoneyama M, Takemura A, Akira S, Taniguchi T, Namiki H, Fujita T (2007) Viral infections activate types I and III interferon genes through a common mechanism. J Biol Chem 282:7576–7581PubMedGoogle Scholar
  76. Orbanz J, Finke S (2010) Generation of recombinant European bat lyssavirus type 1 and inter-genotypic compatibility of lyssavirus genotype 1 and 5 antigenome promoters. Arch Virol 155:1631–1641PubMedGoogle Scholar
  77. Park KH, Huang T, Correia FF, Krystal M (1991) Rescue of a foreign gene by Sendai virus. Proc Natl Acad Sci USA 88:5537–5541PubMedGoogle Scholar
  78. Pattnaik AK, Wertz GW (1990) Replication and amplification of defective interfering particle RNAs of vesicular stomatitis virus in cells expressing viral proteins from vectors containing cloned cDNAs 42. J Virol 64:2948–2957PubMedCentralPubMedGoogle Scholar
  79. Pattnaik AK, Wertz GW (1991) Cells that express all five proteins of vesicular stomatitis virus from cloned cDNAs support replication, assembly, and budding of defective interfering particles. Proc Natl Acad Sci USA 88:1379–1383PubMedGoogle Scholar
  80. Pattnaik AK, Ball LA, LeGrone AW, Wertz GW (1992) Infectious defective interfering particles of VSV from transcripts of a cDNA clone. Cell 69:1011–1020PubMedGoogle Scholar
  81. Perrotta AT, Been MD (1990) The self-cleaving domain from the genomic RNA of hepatitis delta virus: sequence requirements and the effects of denaturant. Nucleic Acids Res 18:6821–6827PubMedCentralPubMedGoogle Scholar
  82. Pfaller CK, Radeke MJ, Cattaneo R, Samuel CE (2013) Measles virus C protein impairs production of defective copyback double-stranded viral RNA and activation of protein kinase R. J Virol. Oct 23. [Epub ahead of print] PubMed PMID: 24155404Google Scholar
  83. Platanias LC (2005) Mechanisms of type-I- and type-II-interferon-mediated signalling. Nat Rev Immunol 5:375–386PubMedGoogle Scholar
  84. Pringle CR (2005) Mononegavirales. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy. Eighth report of the International Committee on the taxonomy of viruses. Elsevier/Academic, London, pp 609–614Google Scholar
  85. Racaniello VR, Baltimore D (1981) Cloned poliovirus complementary DNA is infectious in mammalian cells. Science 214:916–919PubMedGoogle Scholar
  86. Radecke F, Spielhofer P, Schneider H, Kaelin K, Huber M, Dotsch C, Christiansen G, Billeter MA (1995) Rescue of measles viruses from cloned DNA. EMBO J 14:5773–5784PubMedGoogle Scholar
  87. Rajani KR, Pettit Kneller EL, McKenzie MO, Horita DA, Chou JW, Lyles DS (2012) Complexes of vesicular stomatitis virus matrix protein with host Rae1 and Nup98 involved in inhibition of host transcription. PLoS Pathog 8:e1002929PubMedCentralPubMedGoogle Scholar
  88. Randall RE, Goodbourn S (2008) Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures. J Gen Virol 89:1–47PubMedGoogle Scholar
  89. Rieder M, Conzelmann KK (2011) Interferon in rabies virus infection. Adv Virus Res 79:91–114PubMedGoogle Scholar
  90. Rieder M, Brzózka K, Pfaller CK, Cox JH, Stitz L, Conzelmann KK (2011) Genetic dissection of interferon-antagonistic functions of rabies virus phosphoprotein: inhibition of interferon regulatory factor 3 activation is important for pathogenicity. J Virol 85:842–852PubMedCentralPubMedGoogle Scholar
  91. Roberts A, Rose JK (1998) Recovery of negative-strand RNA viruses from plasmid DNAs: a positive approach revitalizes a negative field. Virology 247:1–6PubMedGoogle Scholar
  92. Rothenberg E, Smotkin D, Baltimore D, Weinberg RA (1977) In vitro synthesis of infectious DNA of murine leukaemia virus. Nature (Lond) 269:122–126Google Scholar
  93. Russell SJ, Peng KW, Bell JC (2012) Oncolytic virotherapy. Nat Biotechnol 30:658–670PubMedGoogle Scholar
  94. Sakaguchi T, Kato A, Kiyotani K, Yoshida T, Nagai Y (2008) Studies on the paramyxovirus accessory genes by reverse genetics in the Sendai virus-mouse system. Proc Jpn Acad Ser B Phys Biol Sci 84:439–451PubMedCentralPubMedGoogle Scholar
  95. Schnell MJ, Mebatsion T, Conzelmann KK (1994) Infectious rabies viruses from cloned cDNA. EMBO J 13:4195–4203PubMedGoogle Scholar
  96. Schnell MJ, Buonocore L, Kretzschmar E, Johnson E, Rose JK (1996) Foreign glycoproteins expressed from recombinant vesicular stomatitis viruses are incorporated efficiently into virus particles. Proc Natl Acad Sci USA 93:11359–11365PubMedGoogle Scholar
  97. Schnell MJ, Johnson JE, Buonocore L, Rose JK (1997) Construction of a novel virus that targets HIV-1-infected cells and controls HIV-1 infection. Cell 90:849–857PubMedGoogle Scholar
  98. Schnell MJ, Buonocore L, Boritz E, Ghosh HP, Chernish R, Rose JK (1998) Requirement for a non-specific glycoprotein cytoplasmic domain sequence to drive efficient budding of vesicular stomatitis virus. EMBO J 17:1289–1296PubMedGoogle Scholar
  99. Schoggins JW, Wilson SJ, Panis M, Murphy MY, Jones CT, Bieniasz P, Rice CM (2011) A diverse range of gene products are effectors of the type I interferon antiviral response. Nature (Lond) 472:481–485Google Scholar
  100. Schuhmann KM, Pfaller CK, Conzelmann KK (2011) The measles virus V protein binds to p65 (RelA) to suppress NF-{kappa}B activity. J Virol 85:3162–3171PubMedCentralPubMedGoogle Scholar
  101. Sharma S, tenOever BR, Grandvaux N, Zhou GP, Lin R, Hiscott J (2003) Triggering the interferon antiviral response through an IKK-related pathway. Science 300:1148–1151PubMedGoogle Scholar
  102. Sharmeen L, Kuo MY, Dinter-Gottlieb G, Taylor J (1988) Antigenomic RNA of human hepatitis delta virus can undergo self-cleavage. J Virol 62:2674–2679PubMedCentralPubMedGoogle Scholar
  103. Sparrer KM, Pfaller CK, Conzelmann KK (2012) Measles virus C protein interferes with beta interferon transcription in the nucleus. J Virol 86:796–805Google Scholar
  104. Taniguchi T, Palmieri M, Weissmann C (1978) QB DNA-containing hybrid plasmids giving rise to QB phage formation in the bacterial host. Nature (Lond) 274:223–228Google Scholar
  105. Tao L, Ge J, Wang X, Zhai H, Hua T, Zhao B, Kong D, Yang C, Chen H, Bu Z (2010) Molecular basis of neurovirulence of flury rabies virus vaccine strains: importance of the polymerase and the glycoprotein R333Q mutation. J Virol 84:8926–8936PubMedCentralPubMedGoogle Scholar
  106. Theofilopoulos AN, Baccala R, Beutler B, Kono DH (2005) Type I interferons (alpha/beta) in immunity and autoimmunity. Annu Rev Immunol 23:307–335PubMedGoogle Scholar
  107. Theriault S, Groseth A, Artsob H, Feldmann H (2005) The role of reverse genetics systems in determining filovirus pathogenicity. Arch Virol Suppl 19:157–177PubMedGoogle Scholar
  108. Ugolini G (1995) Specificity of rabies virus as a transneuronal tracer of motor networks: transfer from hypoglossal motoneurons to connected second-order and higher order central nervous system cell groups. J Comp Neurol 356:457–480PubMedGoogle Scholar
  109. Versteeg GA, Garcia-Sastre A (2010) Viral tricks to grid-lock the type I interferon system. Curr Opin Microbiol 13:508–516PubMedCentralPubMedGoogle Scholar
  110. Vulliemoz D, Roux L (2001) “Rule of six:” how does the Sendai virus RNA polymerase keep count? J Virol 75:4506–4518PubMedCentralPubMedGoogle Scholar
  111. Weissmann C, Weber H, Taniguchi T, Muller W, Meyer F (1979) Reversed genetics: a new approach to the elucidation of structure–function relationship. Ciba Found Symp 66:47–61PubMedGoogle Scholar
  112. Whelan SP, Ball LA, Barr JN, Wertz GT (1995) Efficient recovery of infectious vesicular stomatitis virus entirely from cDNA clones. Proc Natl Acad Sci USA 92:8388–8392PubMedGoogle Scholar
  113. Whelan SP, Barr JN, Wertz GW (2004) Transcription and replication of nonsegmented negative-strand RNA viruses. Curr Top Microbiol Immunol 283:61–119PubMedGoogle Scholar
  114. Wickersham IR, Feinberg EH (2012) New technologies for imaging synaptic partners. Curr Opin Neurobiol 22:121–127PubMedGoogle Scholar
  115. Wickersham IR, Lyon DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, Young JA, Callaway EM (2007) Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons. Neuron 53:639–647PubMedCentralPubMedGoogle Scholar
  116. Yoneyama M, Suhara W, Fukuhara Y, Fukuda M, Nishida E, Fujita T (1998) Direct triggering of the type I interferon system by virus infection: activation of a transcription factor complex containing IRF-3 and CBP/p300. EMBO J 17:1087–1095PubMedGoogle Scholar
  117. Yu S, Feng X, Shu T, Matano T, Hasegawa M, Wang X, Li H, Li Z, Zhong R, Zeng Y (2010) Comparison of the expression and immunogenicity of wild-type and sequence-modified HIV-1 gag genes in a recombinant Sendai virus vector. Curr HIV Res 8:199–206PubMedGoogle Scholar

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© Springer Japan 2013

Authors and Affiliations

  1. 1.Max von Pettenkofer-Institute and Gene CenterLudwig-Maximilians-University MunichMuenchenGermany

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