Abstract
Extracellular phosphate (Pi) has been shown to act as a signaling molecule capable of altering gene expression and cellular function during osteoblastic differentiation. In this study, we investigated whether Pi regulates expression of bone morphogenetic protein (BMP)-2, a crucial regulator of osteogenic differentiation, and explored the signaling pathway involved using human dental pulp cells and human periodontal ligament cells. We found that Pi increased BMP-2 gene expression. Experiments using signaling inhibitors revealed that the increase in BMP-2 expression was dependent on the cAMP/protein kinase A signaling pathway. We also showed that Pi activates the ERK1/2, and treatment with PD98059, an ERK1/2 inhibitor, suppressed Pi-mediated BMP-2 increase, indicating that activation of the ERK1/2 pathway is required for this Pi-mediated response. These findings demonstrate a novel ability of Pi to stimulate BMP-2 via cAMP/protein kinase A and ERK1/2 pathways.
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Reference
Tada H, Nemoto E, Foster BL et al (2011) Phosphate increases bone morphogenetic protein-2 expression through cAMP-dependent protein kinase and ERK1/2 pathways in human dental pulp cells. Bone 48:1409–1416
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Nemoto, E., Tada, H., Shimauchi, H. (2012). Extracellular Phosphate Increases Bone Morphogenetic Protein-2 Expression in Human Dental Pulp Cells and Human Periodontal Ligament Cells. In: Sasaki, K., Suzuki, O., Takahashi, N. (eds) Interface Oral Health Science 2011. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54070-0_35
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DOI: https://doi.org/10.1007/978-4-431-54070-0_35
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-54069-4
Online ISBN: 978-4-431-54070-0
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