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The Influence of Mesenchymal Stem Cell Growth Medium for Isolation of STRO-1 Positive Cells from Human Periodontal Ligament

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Interface Oral Health Science 2011

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Abstract

In this study, we examined the influence of mesenchymal stem cells growth medium (MSCGM) for STRO-1 positive cells derived from periodontal ligament (PDL) compared with DMEM.

PDL cells cultured in DMEM showed spindle shape. On the other hand, PDL cells cultured in MSCGM for 7 days showed colony formation. The isolation rate of STRO-1 positive cells in MSCGM was 1.57 times higher than that in DMEM in 7 days culture and 2.49 times higher in 14 days culture.

It is suggested that MSCGM is effective to increase the isolation rate of STRO-1 positive cells from PDL.

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Reference

  1. Nishihara D, Iwamatsu-Kobayashi D, Hirata M, Kindaichi K, Kindaichi J, Komatsu M (2007) Mesenchymal stem cells in human wisdom tooth germs. Interface Oral Health Science:187–188

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Authors and Affiliations

  1. Division of Comprehensive Dentistry, Tohoku University Hospital, 4-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan

    Naoki Endo, Masaaki Iwamatsu & Masahiko Kikuchi

  2. Division of Operative Dentistry, Tohoku University Graduate School of Dentistry, 4-1 Seiryou-machi, Aoba-ku, Sendai, 980-8575, Japan

    Yoko Iwamatsu-Kobayashi, Daisuke Nishihara & Masashi Komatsu

Authors
  1. Naoki Endo
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  2. Yoko Iwamatsu-Kobayashi
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  3. Daisuke Nishihara
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  4. Masaaki Iwamatsu
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  5. Masashi Komatsu
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  6. Masahiko Kikuchi
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Corresponding author

Correspondence to Naoki Endo .

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Editors and Affiliations

  1. Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan

    Keiichi Sasaki D.D.S., Ph.D. (Dean and Professor), Osamu Suzuki Ph.D., M.Eng. (Professor) & Nobuhiro Takahashi D.D.S., Ph.D. (Vice-Dean and Professor) (Dean and Professor),  (Professor) &  (Vice-Dean and Professor)

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Endo, N., Iwamatsu-Kobayashi, Y., Nishihara, D., Iwamatsu, M., Komatsu, M., Kikuchi, M. (2012). The Influence of Mesenchymal Stem Cell Growth Medium for Isolation of STRO-1 Positive Cells from Human Periodontal Ligament. In: Sasaki, K., Suzuki, O., Takahashi, N. (eds) Interface Oral Health Science 2011. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54070-0_28

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  • DOI: https://doi.org/10.1007/978-4-431-54070-0_28

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-54069-4

  • Online ISBN: 978-4-431-54070-0

  • eBook Packages: MedicineMedicine (R0)

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