Visual Screening for the Natural Compounds That Affect the Formation of Nuclear Structures

  • Kaya Shigaki
  • Kazuaki Tokunaga
  • Yuki Mihara
  • Yota Matsuo
  • Yamato Kojimoto
  • Hiroaki Yagi
  • Masayuki Igarashi
  • Tokio Tani
Conference paper


In eukaryotic cells, there are two major compartments separated by the nuclear membrane: the nucleus, where transcription and replication of DNA occur, and the cytoplasm, where translation of mRNAs to proteins occurs. Interestingly, the interphase nucleus is further divided into a dozen subnuclear compartments, such as nucleoli, speckles, Cajal bodies, paraspeckles, promyelocytic leukemia (PML) bodies, and gems (for gemini of Cajal bodies) (Fig. 1) [1].


Alternative Splice High Pressure Liquid Chromatography Splice Factor Cajal Body Nuclear Speckle 



We thank Dr. Masatoshi Hagiwara (Kyoto University) for collaboration and Ms. Maya Umekita (Institute of Microbial Chemistry) for technical assistance. We also thank Hafize Aysin Demirkol, Yutaro Kurogi, and other members of the Tani laboratory for their helpful discussions. This research was supported by grants from the Uehara Foundation, Japan, and Ministry of Education, Culture, Sports, Science and Technology of Japan.


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Copyright information

© Springer 2012

Authors and Affiliations

  • Kaya Shigaki
    • 1
  • Kazuaki Tokunaga
    • 1
  • Yuki Mihara
    • 1
  • Yota Matsuo
    • 1
  • Yamato Kojimoto
    • 1
  • Hiroaki Yagi
    • 1
  • Masayuki Igarashi
    • 2
  • Tokio Tani
    • 1
  1. 1.Department of Biological Sciences, Graduate School of Science and TechnologyKumamoto UniversityKumamotoJapan
  2. 2.Laboratory of Disease BiologyInstitute of Microbial ChemistryTokyoJapan

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