Visual Screening for the Natural Compounds That Affect the Formation of Nuclear Structures
In eukaryotic cells, there are two major compartments separated by the nuclear membrane: the nucleus, where transcription and replication of DNA occur, and the cytoplasm, where translation of mRNAs to proteins occurs. Interestingly, the interphase nucleus is further divided into a dozen subnuclear compartments, such as nucleoli, speckles, Cajal bodies, paraspeckles, promyelocytic leukemia (PML) bodies, and gems (for gemini of Cajal bodies) (Fig. 1) .
KeywordsAlternative Splice High Pressure Liquid Chromatography Splice Factor Cajal Body Nuclear Speckle
We thank Dr. Masatoshi Hagiwara (Kyoto University) for collaboration and Ms. Maya Umekita (Institute of Microbial Chemistry) for technical assistance. We also thank Hafize Aysin Demirkol, Yutaro Kurogi, and other members of the Tani laboratory for their helpful discussions. This research was supported by grants from the Uehara Foundation, Japan, and Ministry of Education, Culture, Sports, Science and Technology of Japan.
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