Advertisement

Establishment of a Novel System for Studying the Syk Function in B Cells

  • Tomohiro Kurosaki
  • Clifford A. Lowell
Conference paper

Abstract

The signal through the B-cell antigen-receptor (BCR) complex, which is composed of the BCR itself and the Igα (CD79a)/Igβ (CD79b) heterodimer, is essential for B-cell development and humoral immune responses. The cytoplasmic domains of both Igα and Igβ have an ITAM (immunoreceptor tyrosine-based activation motif), and tyrosine residues in the ITAM are phosphorylated after BCR stimulation followed by the recruitment and activation of protein tyrosine kinases (PTKs). Two classes of the BCR-associated PTK are now defined: Src-PTK such as Lyn, Fyn, Blk, Lck [1–3], and spleen tyrosine kinase, Syk.

Keywords

Tyrosine Phosphorylation Mutant Kinase Spleen Tyrosine Kinase Small Molecule Kinase Inhibitor Porcine Spleen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Burkhardt AL, Brunswick M, Boten JB, Mond JJ (1991) Anti-immunoglobulin stimulation of B lymphocytes activates src-related protein-tyrosine kinase. Proc Natl Acad Sci USA 88:7410–7413PubMedCrossRefGoogle Scholar
  2. 2.
    Yamanashi Y, Kakiuchi T, Mizuguchi J, Yamamoto T, Toyoshima K (1992) Association of B cell antigen receptor with protein kinase Lyn. Science 251:192–194CrossRefGoogle Scholar
  3. 3.
    Clark MR, Campbell KS, Kazlauskas A, Johnson SA, Herz M, Potter TA, Pleiman C, Cambier JC (1992) The B cell antigen receptor complex: association of Ig-α and Ig-β with distinct cytoplasmic effectors. Science 258:123–125PubMedCrossRefGoogle Scholar
  4. 4.
    Taniguchi T, Kobayashi T, Kondo J, Takahashi K, Nakamura H, Suzuki J, Nagai T, Nakamura S, Yamamura H (1991) Molecular cloning of a porcine gene sykthat encodes a 72-kDa protein-tyrosine kinase showing high susceptibility to proteolysis. J Biol Chem 266:15790–15796PubMedGoogle Scholar
  5. 5.
    Benhamou M, Ryba NJ, Kihara H, Nishikata H, Siraganian RP (1993) Protein-tyrosine kinase p72syk in high affinity IgE receptor signaling: identification as a component of pp 72 and association with the receptor γ chain after receptor aggregation. J Biol Chem 268:23318–23324PubMedGoogle Scholar
  6. 6.
    Greenberg S, Chang P, Silverstein SC (1994) Tyrosine phosphorylation of the γ subunit of Fcγ receptors, p72syk, and paxillin during Fc receptor-mediated phagocytosis in macrophages. J Biol Chem 269:3897–3902PubMedGoogle Scholar
  7. 7.
    Yamada T, Taniguchi T, Yang C, Yasue S, Saito H, Yamamura H (1993) Association with B-cell-antigen receptor with protein-tyrosine kinase p72syk and activation by engagement of membrane IgM. Eur J Biochem 213:455–459PubMedCrossRefGoogle Scholar
  8. 8.
    Fanger NA, Voigtlaender D, Liu C, Swink S, Wardwell K, Fisher J, Graziano RF, Pfefferkorn LC, Guyre PM (1997) Characterization of expression, cytokine regulation, and effector function of the high affinity IgG receptor Fcγ RI (CD64) expressed on human blood dendritic cells. J Immunol 158:3090–3098PubMedGoogle Scholar
  9. 9.
    Reth M, Wienands J (2000) Initiation and processing of signals from B cell antigen receptor. Annu Rev Immunol 15:453–479CrossRefGoogle Scholar
  10. 10.
    Kurosaki T (1999) Genetic analyses of B cell antigen receptor signaling. Annu Rev Immunol 17:555–592PubMedCrossRefGoogle Scholar
  11. 11.
    Takata M, Sabe H, Hata A, Inazu T, Homma Y, Nukada T, Yamamura H, Kurosaki T (1994) Tyrosine kinases Lyn and Syk regulate B cell receptor-coupled Ca2+mobilization through distinct pathways. EMBO J 13:1341–1349PubMedGoogle Scholar
  12. 12.
    Kurosaki T, Takata M, Yamanashi Y, Inazu T, Taniguchi T, Yamamoto T, Yamamura H (1994) Syk activation by the Src-family tyrosine kinase in the B cell receptor signaling. J Exp Med 179:1725–1729PubMedCrossRefGoogle Scholar
  13. 13.
    Nagai K, Takata M, Yamamura H, Kurosaki T (1995) Tyrosine phosphorylation of Shc is mediated through Lyn and Syk in B cell receptor signaling. J Biol Chem 270:6824–6829PubMedCrossRefGoogle Scholar
  14. 14.
    Kurosaki T, Johnson SA, Pao L, Sada K, Yamamura H, Cambier JC (1995) Role of the Syk autophosphorylation site and SH2 domains in B cell antigen receptor signaling. J Exp Med 182:1815–1823PubMedCrossRefGoogle Scholar
  15. 15.
    Pogue SL, Kurosaki T, Bolen J, Herbst R (2000) B cell antigen receptor-induced activation of Akt promotes B cell survival and is dependent on Syk kinase. J Immunol 165:1300–1306PubMedGoogle Scholar
  16. 16.
    Baba Y, Hashimoto S, Matsushita M, Watanabe D, Kishimoto T, Kurosaki T, Tsukada S (2001) BLNK mediates Syk-dependent Btk activation. Proc Natl Acad Sci USA 98:2582–2586PubMedCrossRefGoogle Scholar
  17. 17.
    Goitsuka R, Fujimura Y, Mamada H, Ueda A, Morimura T, Uetsuka K, Doi K, Tsuji S, Kitamura D (1998) BASH, a novel signaling molecule preferentially expressed in B cells of the bursa of Fabricius. J Immunol 161:5804–5808PubMedGoogle Scholar
  18. 18.
    Cheng AM, Rowley B, Pao W, Hayday A, Bolen JB, Pawson T (1995) Syk tyrosine kinase required for mouse viability and B-cell development. Nature (Lond) 378:303–306CrossRefGoogle Scholar
  19. 19.
    Davies SP, Reddy H, Caivano M, Cohen P (2000) Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J 351:95–105PubMedCrossRefGoogle Scholar
  20. 20.
    Shah K, Liu Y, Deirmengian C, Shokat KM (1997) Engineering unnatural nucleotide specificity for Rous sarcoma virus tyrosine kinase to uniquely label its direct substrates. Proc Natl Acad Sci USA 94:3565–3570PubMedCrossRefGoogle Scholar
  21. 21.
    Bishop AC, Shah K, Liu Y, Witucki L, Kung C, Shokat KM (1998) Design of allele-specific inhibitors to probe protein kinase signaling. Curr Biol 8:257–266PubMedCrossRefGoogle Scholar
  22. 22.
    Shokat K, Velleca M (2000) Novel chemical genetic approaches to the discovery of signal transduction inhibitors. Drug Discov Today 7:872–879CrossRefGoogle Scholar
  23. 23.
    Gregan J, Zhang C, Rumpf C, Cipak L, Li Z, Uluocak P, Nasmyth K, Shokat KM (2007) Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe. Nat Protocol 2:2996–3000CrossRefGoogle Scholar
  24. 24.
    Danzel A, Hare KJ, Zhang C, Shokat K, Jenkinson EJ, Anderson G, Hayday A (2003) A chemical genetic system for the analysis of kinases regulating T cell development. J Immunol 171:519–523Google Scholar
  25. 25.
    Weiss EL, Bishop AC, Shokat KM, Drubin DG (2000) Chemical genetic analysis of the ­budding-yeast p21-activated kinase Cla4p. Nat Cell Biol 2:677–685PubMedCrossRefGoogle Scholar
  26. 26.
    Zhang C, Kenski DM, Paulson JL, Bonshtien A, Sessa G, Cross JV, Templeton DJ, Shokat KM (2005) A second-site suppressor strategy for chemical genetic analysis of diverse protein kinases. Nat Methods 2:435–441PubMedCrossRefGoogle Scholar
  27. 27.
    Denzel A, Hare KJ, Zhang C, Shokat K, Jenkinson EJ, Anderson G, Hayday A (2003) Cutting edge: a chemical genetic system for the analysis of kinases regulating T cell development. J Immunol 171:519–523PubMedGoogle Scholar
  28. 28.
    Levin SE, Zhang C, Kadlecek TA, Shokat KM, Weiss A (2008) Inhibition of Zap-70 kinase activity via an analog-sensitive allele blocks T cell receptor and CD28 superagonist signaling. J Biol Chem 283:15419–15430PubMedCrossRefGoogle Scholar
  29. 29.
    Chen X, Ye H, Kuruvilla R, Ramanan N, Scangos KW, Zhang C, Johnson NM, England PM, Shokat KM, Ginty DD (2005) A chemical-genetic approach to studying neurotrophin signaling. Neuron 46:13–21PubMedCrossRefGoogle Scholar
  30. 30.
    Holt JR, Gillespie SK, Provance DW, Shah K, Shokat KM, Corey DP, Mercer JA, Gillespie PG (2002) A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells. Cell 108:371–381PubMedCrossRefGoogle Scholar
  31. 31.
    Miller AL, Zhang C, Shokat KM, Lowell CA (2009) Generation of a novel system for studying spleen tyrosine kinase function in macrophages and B cells. J Immunol 182:988–998PubMedGoogle Scholar

Copyright information

© Springer 2012

Authors and Affiliations

  1. 1.Laboratory for Lymphocyte Differentiation, WPI Immunology Frontier Research CenterOsaka UniversityOsakaJapan
  2. 2.RIKEN Research Center for Allergy and ImmunologyKanagawaJapan
  3. 3.Department of Laboratory MedicineUniversity of CaliforniaSan FranciscoUSA

Personalised recommendations