Identification of Protein–Small Molecule Interactions by Chemical Array

  • Hiroyuki Osada
  • Siro Simizu
Conference paper


Molecular biology techniques have enabled us to understand the structure and function of genes and proteins. However, many phenomena, such as intracellular protein–protein interactions in cells, remain unsolved. If a specific inhibitor of a protein is available, we can describe complex biological systems that have been considered intractable. To identify drug seeds and bioprobes, the RIKEN Natural Products Depository (NPDepo) was developed to use in screens, based on the original chemical library platform, as described here. In this chapter, we introduce a screen for identifying small molecule inhibitors using the newly developed chemical array in NPDepo.


Molecular Biology Technique Chemical Library Complex Biological System Microbial Metabolite Cell Signaling Network 
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Copyright information

© Springer 2012

Authors and Affiliations

  1. 1.Chemical Biology Department, RIKEN Advanced Science InstituteWako, SaitamaJapan
  2. 2.Department of Applied Chemistry, Faculty of Science and TechnologyKeio UniversityYokohamaJapan

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