An Overview of Transcriptome Studies in Non-Human Primates
The transcriptome represents the whole set of transcribed data in a genome. It is an important link between the genotype and phenotype of an organism. Transcriptome studies in non-human primates have been performed in many biomedical and evolutionary studies, which have led to many novel findings. Two major methods for transcriptome analysis summarized here are cDNA cloning and DNA microarrays. Both methods have technical advantages and disadvantages. Generally, cDNA cloning has an advantage for qualitative measurement of transcripts, such as for sequence or structural changes in mRNAs, whereas DNA microarrays have an advantage for quantitative measurement of gene expression levels. New DNA sequencers can identify millions or billions of transcripts, and thus are useful for both qualitative and quantitative measurement of the transcriptome, which will open new fields of transcriptome studies.
KeywordsTranscriptome Study Tiling Array Human Lineage Cynomolgus Macaque Chimpanzee Genome
Expressed sequence tag
Serial analysis of gene expression
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