Characterization of the Interferon Alpha Response Against HCV in Hepatoma Cells
More than 170 million people worldwide are persistently infected with hepatitis C virus (HCV) and an estimated 70% of them suffer from variable and severe liver disease. Combination therapy with interferon alpha (IFN-a) and the nucleoside analogue ribavirin leads to sustained recovery in 45-80% of treated patients. The basis for the observed resistance to IFN therapy is not known. The development of a tissue culture system permissive for HCV replication in Huh7 cells has provided a new tool to investigate the mechanism of antiviral therapies against HCV. As in vivo, HCV RNA amplification in Huh7 cells appears to be inhibited by the antiviral programs induced by IFN-a. In this study we sought to further validate this system and investigated whether HCV replication could interfere with the IFN response.
KeywordsHuh7 Cell Interferon Sensitivity Determine Region Subgenomic Replicon Human Fibrosarcoma Cell Line HT1080 Respective Parental Cell Line
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