Hepatitis C Virus Replication in Cell Culture

  • Michael Frese
  • Volker Lohmann
  • Thomas Pietschmann
  • Artur Kaul
  • Nicole Krieger
  • Jens Bukh
  • Ralf Bartenschlager
Conference paper


By using self-replicating hepatitis C virus (HCV) RNAs, it is now possible to investigate various aspects of the viral replication cycle in cell culture. Here, we describe the construction of subgenomic and genomic replicons which replicate to high levels in the human hepatoma cell line Huh-7. Furthermore, we report and discuss recent contributions from our laboratory which has provided a better understanding of (i) HCV particle formation, (ii) the mechanisms behind the cell culture-adaptation of HCV, and (iii) the innate immune response against HCV.


Internal Ribosome Entry Site Subgenomic Replicon EMCV Internal Ribosome Entry Site Genomic Replicon Lohmann Versus 
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Copyright information

© Springer Japan 2004

Authors and Affiliations

  • Michael Frese
    • 1
  • Volker Lohmann
    • 1
  • Thomas Pietschmann
    • 1
  • Artur Kaul
    • 1
  • Nicole Krieger
    • 1
  • Jens Bukh
    • 2
  • Ralf Bartenschlager
    • 1
  1. 1.Abteilung Molekulare Virologie, Hygiene InstitutUniversität HeidelbergHeidelbergGermany
  2. 2.Hepatitis Viruses Section, Laboratory of Infectious DiseasesNational Institute of Allergy and Infectious DiseasesBethesdaUSA

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