Hepatitis C Virus Replication in Cell Culture
By using self-replicating hepatitis C virus (HCV) RNAs, it is now possible to investigate various aspects of the viral replication cycle in cell culture. Here, we describe the construction of subgenomic and genomic replicons which replicate to high levels in the human hepatoma cell line Huh-7. Furthermore, we report and discuss recent contributions from our laboratory which has provided a better understanding of (i) HCV particle formation, (ii) the mechanisms behind the cell culture-adaptation of HCV, and (iii) the innate immune response against HCV.
KeywordsInternal Ribosome Entry Site Subgenomic Replicon EMCV Internal Ribosome Entry Site Genomic Replicon Lohmann Versus
Unable to display preview. Download preview PDF.
- 3.World Health Organization. Hepatitis C: global prevalence (update). Wkly Epidemiol Rec 2000;75:18–19.Google Scholar
- 5.Theodore D, Fried MW. Natural history and disease manifestations of hepatitis C infection. Curr Top Microbiol Immunol 2000;242:44–54.Google Scholar
- 17.Goodbourn S, Didcock L, Randall RE. Interferons: cell signalling, immune modulation, antiviral response and virus countermeasures. J Gen Virol 2000;81:23412364.Google Scholar