Characterization of Potassium Channels from Arabidopsis thaliana
Potassium ion (K+) is the most abundant cation inside prokaryotic and eukaryotic cells and the K+ transport system in biological membranes is essential for maintenance of membrane potential and diverse cellular processes in all organisms studied thus far. Bacterial K+ uptake systems have been investigated extensively. In E. coli, three kinds of K+ transporters have been identified as major K+ transport uptake systems. E. coli Kdp is a P-type ATPase which has been classified as a high-affinity K+ transport system. The Kup transporter in E. coli is a low affinity type of K+ uptake transporter and the Trk system is the third K+ transport system. In fact, an E. coli mutant, lacking the three K+ uptake systems, Trk, Kup, and Kdp, could not grow on K+-limiting medium. In Arabidopsis plants, the K+ channels proteins exist . More than ten genes encoding the homologous channels are dispersed in all of the genome and these homologous channels are expressed in Eucalyptus. We isolated the gene encoding the novel K+ channel from Arabidopsis and characterized the function using E. coli expression system.
KeywordsUptake System Coli Mutant Diverse Cellular Process Functional Channel Abundant Cation
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