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Characterization of Potassium Channels from Arabidopsis thaliana

  • Tatsuki Ibuki
  • Yukiko Tokida
  • Nobuyuki Matsuda
  • Katrin Czempinski
  • Bernd Muller-Roeber
  • Toshihiro Ona
  • Nobuyuki Uozumi
Conference paper

Abstract

Potassium ion (K+) is the most abundant cation inside prokaryotic and eukaryotic cells and the K+ transport system in biological membranes is essential for maintenance of membrane potential and diverse cellular processes in all organisms studied thus far. Bacterial K+ uptake systems have been investigated extensively. In E. coli, three kinds of K+ transporters have been identified as major K+ transport uptake systems. E. coli Kdp is a P-type ATPase which has been classified as a high-affinity K+ transport system. The Kup transporter in E. coli is a low affinity type of K+ uptake transporter and the Trk system is the third K+ transport system. In fact, an E. coli mutant, lacking the three K+ uptake systems, Trk, Kup, and Kdp, could not grow on K+-limiting medium. In Arabidopsis plants, the K+ channels proteins exist [1]. More than ten genes encoding the homologous channels are dispersed in all of the genome and these homologous channels are expressed in Eucalyptus. We isolated the gene encoding the novel K+ channel from Arabidopsis and characterized the function using E. coli expression system.

Keywords

Uptake System Coli Mutant Diverse Cellular Process Functional Channel Abundant Cation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. [1]
    Uozumi, N., Am. J. Physiol. 281 (2001) C733.Google Scholar
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    Tolema, N., Bakker EP., Suzuki A. and Nakamura, T., FEBS Let. 450 (1999) 217.CrossRefGoogle Scholar
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    Uozumi, N., Yamada, K., Goshima, S., Ona, T. and Oiki, S. J. Membrane Biol. 181 (2001) 163.Google Scholar
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    Sato, Y., Sakaguchi, M., Goshima, S., Nakamura, T. and Uozumi, N. J. Biol. Chem. 278 (2003) 13227.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2004

Authors and Affiliations

  • Tatsuki Ibuki
    • 1
  • Yukiko Tokida
    • 1
  • Nobuyuki Matsuda
    • 1
  • Katrin Czempinski
    • 2
    • 3
  • Bernd Muller-Roeber
    • 2
    • 3
  • Toshihiro Ona
    • 4
  • Nobuyuki Uozumi
    • 1
  1. 1.Bioscience and Biotechnology CenterNagoya UniversityNagoya, AichiJapan
  2. 2.Institut für Biochemie und BiologieUniversität PotsdamGolmGermany
  3. 3.Cooperative Research Group of the Max-Planck-Institute of Molecular Plant PhysiologyGolmGermany
  4. 4.Department of Forest and Forest Products Sciences Graduate School of Bioresource and Bioenvironmental SciencesKyushu UniversityHigashi-ku, FukuokaJapan

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