Potential Application of Pollen Genotyping for Evolutionary Genetic and Genomic Studies: Linkage/Recombination Analysis and Haplotype Sequencing

Part of the Ecological Research Monographs book series (ECOLOGICAL)


Single-pollen genotyping has mainly been used in ecological studies to date. Here, we discuss how this technique would also be valuable for evolutionary genetic and genomic studies. First, we briefly review previous genetic studies that have exploited haploid cells other than single pollen grains. Examples include the sperm genotyping of humans and livestock for recombination analysis and the linkage map of conifer trees using a multicellular haploid female megagametophyte. Single-pollen genotyping would allow similar analysis in angiosperms. We also discuss the advantages and disadvantages of two potential applications: linkage/recombination analysis and haplotype sequencing. These applications would provide more information about the genetic background of “nonmodel” organisms with little prior genomic information and a better understanding of evolutionary and ecological processes.


Amplify Fragment Length Polymorphism Segregation Distortion Single Pollen Haploid Cell Recombination Analysis 


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Copyright information

© Springer 2011

Authors and Affiliations

  1. 1.School of Advanced Sciences, The Graduate University for Advanced StudiesKanagawaJapan
  2. 2.Evolutionary Functional Genomics, Institute of Plant BiologyUniversity of ZurichZurichSwitzerland

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