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Potential Application of Pollen Genotyping for Evolutionary Genetic and Genomic Studies: Linkage/Recombination Analysis and Haplotype Sequencing

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Single-Pollen Genotyping

Part of the book series: Ecological Research Monographs ((ECOLOGICAL))

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Abstract

Single-pollen genotyping has mainly been used in ecological studies to date. Here, we discuss how this technique would also be valuable for evolutionary genetic and genomic studies. First, we briefly review previous genetic studies that have exploited haploid cells other than single pollen grains. Examples include the sperm genotyping of humans and livestock for recombination analysis and the linkage map of conifer trees using a multicellular haploid female megagametophyte. Single-pollen genotyping would allow similar analysis in angiosperms. We also discuss the advantages and disadvantages of two potential applications: linkage/recombination analysis and haplotype sequencing. These applications would provide more information about the genetic background of “nonmodel” organisms with little prior genomic information and a better understanding of evolutionary and ecological processes.

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Authors and Affiliations

  1. School of Advanced Sciences, The Graduate University for Advanced Studies, Hayama, Kanagawa, 240-0193, Japan

    Yayoi Takeuchi

  2. Evolutionary Functional Genomics, Institute of Plant Biology, University of Zurich, Zurich, Switzerland

    Yayoi Takeuchi & Kentaro K. Shimizu

Authors
  1. Yayoi Takeuchi
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  2. Kentaro K. Shimizu
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Correspondence to Yayoi Takeuchi .

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Editors and Affiliations

  1. Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwakecho, Sakyo, Kyoto, 606-8502, Japan

    Yuji Isagi Ph.D (Professor) (Professor)

  2. Graduate School of Agricultural Science, Tohoku University, Naruko-onsen, Osaki, Miyagi, 989-6711, Japan

    Yoshihisa Suyama Ph.D (Associate Professor) (Associate Professor)

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Takeuchi, Y., Shimizu, K.K. (2011). Potential Application of Pollen Genotyping for Evolutionary Genetic and Genomic Studies: Linkage/Recombination Analysis and Haplotype Sequencing. In: Isagi, Y., Suyama, Y. (eds) Single-Pollen Genotyping. Ecological Research Monographs. Springer, Tokyo. https://doi.org/10.1007/978-4-431-53901-8_9

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