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Single-Pollen Genotyping of Holocene Lake Sediments

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Single-Pollen Genotyping

Part of the book series: Ecological Research Monographs ((ECOLOGICAL))

Abstract

In this study, we present a DNA genotyping method of fossil pollen of Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies (L.) Karst) extracted from Holocene lake sediments (from the past 11,500 years). Genetic analysis of pollen of different ages extracted from different lake sediments allows direct evaluation of the history and dynamics of genotypes in ancient plant taxa and the microevolutionary processes that occurred in the recent past, a method that greatly contributes toward elucidating plant movement in space and time. However, genotyping ancient material is complicated, with several technical difficulties, and requires careful execution and practical expertise.

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Correspondence to Laura Parducci .

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Parducci, L., Suyama, Y. (2011). Single-Pollen Genotyping of Holocene Lake Sediments. In: Isagi, Y., Suyama, Y. (eds) Single-Pollen Genotyping. Ecological Research Monographs. Springer, Tokyo. https://doi.org/10.1007/978-4-431-53901-8_8

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