Single-Pollen Genotyping of Holocene Lake Sediments

  • Laura Parducci
  • Yoshihisa Suyama
Part of the Ecological Research Monographs book series (ECOLOGICAL)


In this study, we present a DNA genotyping method of fossil pollen of Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies (L.) Karst) extracted from Holocene lake sediments (from the past 11,500 years). Genetic analysis of pollen of different ages extracted from different lake sediments allows direct evaluation of the history and dynamics of genotypes in ancient plant taxa and the microevolutionary processes that occurred in the recent past, a method that greatly contributes toward elucidating plant movement in space and time. However, genotyping ancient material is complicated, with several technical difficulties, and requires careful execution and practical expertise.


Multiplex Polymerase Chain Reaction Fossil Pollen Polymerase Chain Reaction Tube Microevolutionary Process Multiplex Polymerase Chain Reaction Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Chamberlain JS, Gibbs RA, Ranier JE, Nguyen PN, Caskey CT (1988) Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acids Res 16:11141–11156CrossRefPubMedGoogle Scholar
  2. Cooper A, Lalueza-Fox C, Anderson S, Rambaut A, Austin J, Ward R (2001) Complete mitochondrial genome sequences of two extinct moas clarify ratite evolution. Nature (Lond) 409:704–707CrossRefGoogle Scholar
  3. Faegri K, Iversen J (1989) Textbook of pollen analysis. Wiley, ChichesterGoogle Scholar
  4. Gilbert MTP, Kivisild T, Gronnow B et al (2008) Paleo-Eskimo mtDNA genome reveals matrilineal discontinuity in Greenland. Science 320:1787–1789CrossRefPubMedGoogle Scholar
  5. Hadly EA, Ramakrishnan U, Chan YL et al (2004) Genetic response to climatic change: insights from ancient DNA and phylochronology. PLoS Biol 2:1600–1609CrossRefGoogle Scholar
  6. Hein J, Schierup MH, Wiuf C (2005) Gene genealogies, variation and evolution. A primer in coalescent theory. Oxford University Press, OxfordGoogle Scholar
  7. Krings M, Stone A, Schmitz RW, Krainitzki H, Stoneking M, Pääbo S (1997) Neanderthal DNA sequences and the origin of modern humans. Cell 90:19–30CrossRefPubMedGoogle Scholar
  8. Lambert DM, Ritchie PA, Millar CD, Holland B, Drummond AJ, Baroni C (2002) Rates of evolution in ancient DNA from Adelie penguins. Science 295:2270–2273CrossRefPubMedGoogle Scholar
  9. Lascoux M, Palmé AE, Cheddadi R, Latta RG (2004) Impact of ice ages on the genetic structure of trees and shrubs. Philos Trans R Soc Lond B 359:197–207CrossRefGoogle Scholar
  10. Matsuki Y, Isagi Y, Suyama Y (2007) The determination of multiple microsatellite genotypes and DNA sequences from a single pollen grain. Mol Ecol Notes 7:194–198CrossRefGoogle Scholar
  11. Montiel R, Malgosa A, Francalacci P (2001) Authenticating ancient human mitochondrial DNA. Hum Biol 73:689–713CrossRefPubMedGoogle Scholar
  12. Nagata N, Saito C, Sakai A, Kuroiwa H, Kuroiwa T (1999) The selective increase or decrease of organellar DNA in generative cells just after pollen mitosis one controls cytoplasmic inheritance. Planta (Berl) 209:53–65CrossRefGoogle Scholar
  13. Paffetti D, Vettori C, Caramelli D, Vernesi C, Lari M, Paganelli A, Paule L, Giannini R (2007) Unexpected presence of Fagus orientalis complex in Italy as inferred from 45,000-year-old DNA pollen samples from Venice lagoon. BMC Evol Biol 16 (suppl 2):S6CrossRefGoogle Scholar
  14. Parducci L, Suyama Y, Lascoux M, Bennett KD (2005) Ancient DNA from pollen: a genetic record of plant population history. Mol Ecol 14:2873–2882CrossRefPubMedGoogle Scholar
  15. Ramakrishnan U, Hadly EA (2009) Using phylochronology to reveal cryptic population histories: review and synthesis of 29 ancient DNA studies. Mol Ecol 18:1310–1330CrossRefPubMedGoogle Scholar
  16. Shapiro B, Drummond AJ, Rambaut A et al (2004) Rise and fall of the Beringian steppe bison. Science 306:1561–1565CrossRefPubMedGoogle Scholar
  17. Suyama Y, Kawamuro K, Kinoshita I, Yoshimura K, Tsumura Y, Takahara H (1996) DNA sequence from a fossil pollen of Abies spp. from Pleistocene peat. Genes Genet Syst 71:145–149CrossRefPubMedGoogle Scholar
  18. Tollefsrud MM, Sønstebø JH, Brochmann C et al (2008) Genetic consequences of glacial survival and postglacial colonization in Norway spruce: combined analysis of mitochondrial DNA and fossil pollen. Mol Ecol 17:4134–4150CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2011

Authors and Affiliations

  1. 1.Department of Ecology and Genetics, Evolutionary Biology CentreUppsala UniversityUppsalaSweden
  2. 2.Laboratory of Forest Ecology, Graduate School of Agricultural ScienceTohoku UniversityNaruko-onsenJapan

Personalised recommendations