Procedure for Single-Pollen Genotyping
Direct determination of multilocus genotypes and DNA sequences from single pollen grains is an efficient method and can be used to answer questions in different research areas such as pollination ecology and genomics. The essential prerequisites to perform this technique are the ability (1) to manipulate small objects in small amounts of liquid by using standard pipettes, (2) to determine optimal conditions to minimize the loss of DNA, (3) to identify the optimal conditions for polymerase chain reaction amplification using an extremely low copy template, and (4) to eliminate possible contaminants. Owing to its simple yet efficient approach, the single-pollen genotyping technique has become the technique of choice among molecular ecologists. This technique is expected to be used extensively in various disciplines in the future.
KeywordsPolymerase Chain Reaction Hybrid Zone Individual Pollen Pollination Ecology Polymerase Chain Reaction Tube
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