Significance of Single-Pollen Genotyping in Ecological Research
Pollination is one of the most important processes of sexual reproduction in angiosperms and gymnosperms. Although the small size of pollen grains precludes direct observation of pollination, rapid advances in molecular genetics allow direct genotyping of a single pollen grain: single-pollen genotyping. The genetic information obtained from a single pollen grain facilitates a variety of ecological analyses, including determination of parentage, gene flow, plant fitness, and genetic diversity. Further, genotyping of DNA from ancient pollen can reveal the process of migration, microevolution, and demographic history of the plants. Single-pollen genotyping for the haploid genome would facilitate recombination analysis and linkage map construction, which is easier than conventional pedigree analysis of inbred lines obtained from experimental crossing. Moreover, genotyping of a single pollen grain allows easier DNA sequencing of the nuclear loci.
KeywordsGene Flow Sexual Reproduction Pollen Movement Pollen Transfer Genetic Trait
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