Abstract
Background: Recent studies suggest that bone marrow cells contribute to neointimal formation after vascular injury. However, the relationship between the inflammatory reactions and bone marrow cell invasion has not been clarified. Method and Results: We insert a large wire (0.38 mm in a diameter) into the femoral arteries of 6–8 week-old male balb/c (WT) and TNF-α Knockout (KO) mice. In imunohistochmistry using CD34 at 1 week, positive cells possibly containing bone marrow derived cells, were hardly observed in KO, but some were observed in WT in neointima. At 4 weeks, CD45 positive cells were rarely seen, and α-smooth muscle actin positive cells were main component of thickened neointima in both groups. In morphometric analysis at 4 weeks after the injury, developed neointimal area was smaller in KO. Furthermore, re-endothelialization appeared earlier in KO than WT. Conclusion: TNF-α is involved in neointimal formation after vascular injury, possible through its inflammatory effects to induce bone marrow cells.
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Shoji, M. et al. (2007). TNF-α mobilizes bone marrow derived cells to vascular wall, resulting in neointima formation through its inflammatory effects. In: Kusano, M., Shioda, S. (eds) New Frontiers in Regenerative Medicine. Springer, Tokyo. https://doi.org/10.1007/978-4-431-38208-9_10
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DOI: https://doi.org/10.1007/978-4-431-38208-9_10
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