Spinal Cord Lesions in Spinal Hyperostotic Mouse (twy/twy) Simulating Ossification of the Posterior Longitudinal Ligament of the Cervical Spine
In the spinal hyperostotic mouse model (twy/twy), at the C1-C2 level developing calcification and ossification, the area of neuronal soma and the length of the neurites significantly decreased with a decrement in the motoneuron population. The compression significantly decreased the expression levels of BDNF and NT-3, trkB, and trkC compared with the levels in adjacent, less-compressed segments. On the other hand, at other spinal cord segments sustaining less compressive stress, enlargement of the neuronal soma and elongation of neurites were observed in association with increased expression of BDNF, NT-3, and the receptor proteins trkB and trkC. In 20-week-old twy mice, separation of the myelin sheath from the axon and axonal swelling with deformation were signifi cant in association of increased mmunoreactivity to neurofi lament protein and growth-associated protein 43. Targeted retrograde adenovirus-BDNF-gene in vivo delivery via the sternomastoid muscle prevented loss of anterior horn neurons at the site of spinal cord compression, enhanced the expression of BDNF, and increased the activities of choline acetyltransferase and acetylcholine esterase in motoneurons of the twy mouse spinal cord.
KeywordsSpinal Cord Spinal Cord Lesion Posterior Longitudinal Ligament Spinal Cord Segment Neuronal Soma
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