Breakdown and Reformation of the Nuclear Envelope
In eukaryotes, the nuclear envelope encapsulates chromosomes and provides a physical framework for their organization; it also acts as a nucleo-cytoplasmic boundary for intracellular components providing a regulated chemical environment within the nucleus. The genetic activities of chromosomes are modulated within this distinct physicochemical domain. The nuclear envelope is an apparently stable structure during interphase in the cell cycle, but is dynamic during mitosis, proceeding through disassembly and reassembly in a short period of time. These processes must be precisely regulated to ensure proper progression of the cell cycle, and defects in such processes often cause cell death or disease. Owing to the advancement of imaging technologies, the dynamic behavior of the nuclear envelope during the cell cycle is now being studied in detail in the living cells of many organisms. In this chapter we describe the dynamic processes of disassembly and reassembly of the nuclear envelope as revealed by fluorescence microscopy.
KeywordsNuclear Envelope Mitotic Spindle Nuclear Pore Complex Fluorescence Recovery After Photobleaching Nuclear Lamina
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