Effects of 5-Bromodeoxyuridine on Chromatin Structure
Normal human cells in culture enter senescence upon passages or treatment with various agents. Among the treatments, 5-bromodeoxyuridine (BrdU) is unique in that it most clearly induces a senescence-like phenomenon in every type of mammalian cells (Michishita et al. 1999). This system ensures genetic and biochemical approaches to study cellular senescence. In contrast, normal cells stop proliferation by stochastic accumulation of senescent cells over a long period of culture. 5-Bromodeoxyuridine is well known as a modulator of cellular differentiation. Therefore, studies of the molecular basis for BrdU facilitate understanding of chromatin-mediated cellular differentiation and cellular senescence.
KeywordsCellular Senescence Nuclear Matrix Senescent Cell Nucleosome Assembly Position Effect Variegation
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