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Visual Biology of Nuclear Dynamics: From Micro- to Nano-dynamics of Nuclear Components

  • Shige H. Yoshimura

Abstract

When you look at an interphase nucleus in a living cell through a light microscope, you will see a round, static organelle separated from the cytoplasm. If you continue the live cell observation, you will easily learn that the cell nucleus does not undergo any significant morphological changes until it reaches the mitosis, where the nuclear envelope and the chromosomes show dynamic structural changes. Because of these morphological properties, the cell nucleus had previously been considered a “container” of genome that provides an enclosed space for genomic events to be carried out. However, recent progress in molecular and cellular biological approaches has led to the revelation that the cell nucleus is composed of various kinds of different “compartments,” each of which is supposed to have a distinct “structure” and “function.” These include promeyelocytic leukemia (PML) bodies, Cajal bodies, nucleolus, nuclear speckles, and nuclear foci (see figure in the Preface). Recent developments in various fluo-rescence observation techniques have revealed that these compartments are moving within a nucleus and there is a constant flow of proteins between nucleoplasm and these compartments (Fig. 1).In this chapter, therecent progress in various “visualization techniques” will be reviewed and how these techniques have been utilized to visualize the structures and the dynamics of the inner nuclear compartments and chromosomes will be described.

Keywords

Atomic Force Microscopy Fluorescent Resonance Energy Transfer Nuclear Pore Complex Fluorescence Recovery After Photobleaching Chromosome Territory 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer 2007

Authors and Affiliations

  • Shige H. Yoshimura
    • 1
  1. 1.Graduate School of BiostudiesKyoto UniversityKyotoJapan

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