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Direct Gene Transfer into Plant Mature Seeds via Electroporation After Vacuum Treatment

  • Takashi Hagio

A number of direct gene transfer methods have been used successfully in plant genetic engineering, providing powerful tools to investigate fundamental and applied problems in plant biology (Chowrira et al., 1996; D'halluin et al., 1992; Morandini and Salamini, 2003; Rakoczy-Trojanowska, 2002; Songstad et al., 1995). In cereals, several methods have been found to be suitable for obtaining transgenic plant; these include bombardment of scutellum (Hagio et al., 1995) and inflorescence cultures (He et al., 2001), and silicon carbide fiber-mediated DNA delivery (Asano et al., 1991) and Agrobacterium tumefaciens transformation (Potrykus, 1990). Electroporation of cereal protoplasts also has proved successful but it involves prolonged cell treatments and generally is limited by the difficulties of regeneration from cereal protoplast cultures (Fromm et al., 1987). Many laboratories worldwide are now using Agrobacterium as a vehicle for routine production of transgenic crop plants. The primary application of the particle system (Klein et al., 1987) has been for transformation of species recalcitrant to conventional Agrobacterium (Binns, 1990) or protoplast methods. But these conventional methods can be applied to the species and varieties that are amenable to tissue culture (Machii et al., 1998). Mature seeds are readily available and free from the seasonal limits that immature embryo, inflorescence, and anther have. This method enables us to produce transgenic plants without time-consuming tissue culture process.

Keywords

Transgenic Plant Mature Seed Immature Embryo Molecular Biology Letter Vacuum Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Asano, Y., Otsuki, Y., Ugaki, M. (1991) Electroporation-mediated and silicon carbide fiber-mediated DNA delivery in Agrostis alba L. (Redtop). Plant Science 79, 247–252.CrossRefGoogle Scholar
  2. Binns, A. N. (1990) Agrobacterium-mediated gene delivery and the biology of host range limitations. Physiologia Plantarum 79, 135–139.CrossRefGoogle Scholar
  3. Chawla, H. S. (2002) Antibiotic Resistance Markers: Introduction to Plant Biotechnology, 2nd edition. Scientific Publishers, Enfield NH, USA 362–363.Google Scholar
  4. Chowrira, G. M., Akella, V., Fuerst, P. E., Lurquin, P. L. (1996) Transgenic grain legumes obtained by in planta electroporation-mediated gene transfer. Molecular Biotechnology 5, 85–96.CrossRefGoogle Scholar
  5. D'halluin, K., Bonne, E., Bossut, M., Beuckeleer, M. D., Leemans, L. (1992) Transgenic maize plants by tissue electroporation. The Plant Cell 4, 1495–1505.CrossRefGoogle Scholar
  6. FAO (Food and Agriculture Organization of the United Nations) (2002) Bulletin of Statistics 2, 20–23.Google Scholar
  7. Fromm, M., Callis, J., Taylor, L. P., Walbot, V. (1987) Electroporation of DNA and RNA into plant protoplasts. Methods in Enzymology 153, 351–382.CrossRefGoogle Scholar
  8. Hagio, T., Hirabayashi, T., Machii, H., Tomotsune, H. (1995) Production of fertile transgenic barley (Hordeum vulgare L.) plant using the hygromycin-resistance marker. Plant Cell Reports 14, 329–334.CrossRefGoogle Scholar
  9. He, G. Y., Lazzeri, P. A., Cannell, M. E. (2001) Fertile transgenic plants obtained from Tritordeum inflorescences by tissue electroporation. Plants Cell Reports 20, 67–72.CrossRefGoogle Scholar
  10. Jefferson, R. A. (1987) Assaying chimeric genes in plants: The GUS gene fusion system. Plant Molecular Biology Reporter 5, 387–405.CrossRefGoogle Scholar
  11. Jefferson, R. A., Kavanagh, T. A., Bevan, M. W. (1987) GUS fusions: βGlucuronidase as a sensitive and versatile gene fusion marker in higher plants. The EMBO Journal 6, 3901–3907.Google Scholar
  12. Klein, T. M., Wolf, E. D., Wu, R., Sanford, J. C. (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327, 70–73.CrossRefGoogle Scholar
  13. Machii, H., Mizuno H., Hirabayashi, T., Li, H., Hagio, T. (1998) Screening wheat genotypes for high callus induction and regeneration capability from anther and immature embryo cultures. Plant Cell Tissue and Organ Culture 53, 67–74.CrossRefGoogle Scholar
  14. Morandini, P., Salamini, F. (2003) Plant biotechnology and breeding: Allied for years to come. Trends in Plant Science 8, 2, 70–75.CrossRefGoogle Scholar
  15. Potrykus, I. (1990) Gene transfer to cereals: An assessment. BIO/TECHNOLOGY 8(6), 535–542.CrossRefGoogle Scholar
  16. Rakoczy-Trojanowska, M. (2002) Alternative methods of plant transformation – A short review. Cellular & Molecular Biology Letters 7, 849–858.Google Scholar
  17. Sinclair, T.R., Purcell, L. C., Sneller, C. H. (2004) Crop transformation and the challenge to increase yield potential. Trends in Plant Science 9, 2, 70–75.CrossRefGoogle Scholar
  18. Songstad, D. D. Somers, D. A., Griesbach, R. J. (1995) Advances in alternative DNA delivery techniques. Plant Cell Tissue Organ Culture 40, 1–15.CrossRefGoogle Scholar
  19. Sorokin, A.P., Ke, X.Y., Chen, D.F., Elliot, M.C. (2000) Production of fertile transgenic wheat plants via tissue electroporation. Plant Science 156, 227–233.CrossRefGoogle Scholar
  20. Ugaki, M., Ueda, T., Timmermans, C. P., Vieira, J., Elliston, K.O., Messing, J. (1991) Replication of a geminivirus-derived shuttle vector in maize endosperm cells. Nucleic Acid Research 19, 371–377.CrossRefGoogle Scholar
  21. Walden, R., Wingender, R. (1995) Gene-transfer and plant regeneration techniques. Trends in Biotechnology 13, 324–331.CrossRefGoogle Scholar

Copyright information

© Springer 2009

Authors and Affiliations

  1. 1.National Institute of Agrobiological SciencesTsukubaJapan

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