Retinal Fiber Tracing by In Ovo Electroporation
Axonal tracing techniques are the fundamentals for the investigation of neural circuit formation. In ovo electroporation system allows us to transfect a gene of interest to the desired place in chick embryos (Odani et al., 2008). Recently, Tol2 transposase element, which was originally found in medaka fish (Koga et al., 1996), has been adapted to an in ovo electroporation system (Niwa et al., 1991; Kawakami et al., 1998, 2000, 2004a, 2004b; Kawakami & Noda, 2004; Kawakami, 2005, 2007; Sato et al., 2007). This system assures the integration of the transgene into the genome by electroporation (Niwa et al., 1991; Sato et al., 2007). We applied this system for tracing retinal fibers (Harada et al., 2008). In this chapter, we demonstrate the method of tracing retinal fibers from both small and large groups of the retinal ganglion cell (RGC) with transposon-mediated gene transfer by in ovo electroporation to chick embryos.
KeywordsChick Embryo Optic Tectum Otic Vesicle Optic Vesicle Medaka Fish
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