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Methods of Matrix Application

  • Yuki Sugiura
  • Mitsutoshi Setou
  • Daisuke Horigome

Abstract

The matrix application procedure is the most critical step of the sample preparation procedure, because it affects co-cystalization of the matrix-analyte complex formed on the tissue surface, thus the quality of the mass spectra and the subsequent imaging results. Several methods have been used for matrix application: (1) manually spraying the matrix solution with a nebulizer sprayer, (2) automatically depositing an array of small droplets of matrix solution with robotic devices, and (3) sublimating the matrices under reduced pressure and elevated temperature. In this chapter, we introduce an overview of these procedures and then continue with details of technical points, especially of methods (1) and (2). Lastly, a Further advanced application method, which is the spray-droplet method to increase signal sensitivity, is also described.

Keywords

Matrix Solution Imaging Mass Spectrometry Sinapinic Acid Matrix Crystal Airtight Container 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 2010

Authors and Affiliations

  • Yuki Sugiura
    • 1
    • 2
  • Mitsutoshi Setou
    • 2
  • Daisuke Horigome
    • 3
  1. 1.Department of Bioscience and BiotechnologyTokyo Institute of TechnologyYokohamaJapan
  2. 2.Department of Molecular AnatomyHamamatsu University School of MedicineHamamatsuJapan
  3. 3.Mitsubishi Kagaku Institute of Life SciencesMachidaJapan

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