Methods of Matrix Application

  • Yuki Sugiura
  • Mitsutoshi Setou
  • Daisuke Horigome


The matrix application procedure is the most critical step of the sample preparation procedure, because it affects co-cystalization of the matrix-analyte complex formed on the tissue surface, thus the quality of the mass spectra and the subsequent imaging results. Several methods have been used for matrix application: (1) manually spraying the matrix solution with a nebulizer sprayer, (2) automatically depositing an array of small droplets of matrix solution with robotic devices, and (3) sublimating the matrices under reduced pressure and elevated temperature. In this chapter, we introduce an overview of these procedures and then continue with details of technical points, especially of methods (1) and (2). Lastly, a Further advanced application method, which is the spray-droplet method to increase signal sensitivity, is also described.


Matrix Solution Imaging Mass Spectrometry Sinapinic Acid Matrix Crystal Airtight Container 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Aerni HR, Cornett DS, Caprioli RM (2006) Automated acoustic matrix deposition for MALDI sample preparation. Anal Chem 78:827–834PubMedCrossRefGoogle Scholar
  2. 2.
    Sugiura Y, Shimma S, Setou M (2006) Two-step matrix application technique to improve ionization efficiency for matrix-assisted laser desorption/ionization in imaging mass spec-trometry. Anal Chem 78:8227–8235PubMedCrossRefGoogle Scholar
  3. 3.
    Hankin JA, Barkley RM, Murphy RC (2007) Sublimation as a method of matrix application for mass spectrometric imaging. J Am Soc Mass Spectrom 18:1646–1652PubMedCrossRefGoogle Scholar
  4. 4.
    Puolitaival SM, Burnum KE, Cornett DS, et al. (2008) Solvent-free matrix dry-coating for MALDI imaging of phospholipids. J Am Soc Mass Spectrom 19:882–886PubMedCrossRefGoogle Scholar
  5. 5.
    Agar N Y, Yang HW, Carroll RS, et al. (2007) Matrix solution fixation: histology-compatible tissue preparation for MALDI mass spectrometry imaging. Anal Chem 79:7416–7423PubMedCrossRefGoogle Scholar
  6. 6.
    Benabdellah F, Touboul D, Brunelle A, et al. (2009) In situ primary metabolites localization on a rat brain section by chemical mass spectrometry imaging. Anal. Chem., 81 (13), 5557–5560PubMedCrossRefGoogle Scholar
  7. 7.
    Sugiura Y, Konishi Y, Zaima N, et al. (2009) Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry. J. Lipid Res. 50: 1776–1788PubMedCrossRefGoogle Scholar
  8. 8.
    Shimma S, Furuta M, Ichimura K, et al. (2006) A novel approach to in situ proteome analysis using chemical inkjet printing technology and MALDI-QIT-TOF tandem mass spectrometer. J Mass Spectrom Soc Jpn 54:133–140CrossRefGoogle Scholar
  9. 9.
    Meistermann H, Norris JL, Aerni HR, et al. (2006) Biomarker discovery by imaging mass spectrometry: transthyretin is a biomarker for gentamicin-induced nephrotoxicity in rat. Mol Cell Proteomics 5:1876–1886PubMedCrossRefGoogle Scholar
  10. 10.
    Groseclose MR, Andersson M, Hardesty WM, Caprioli RM (2007) Identification of proteins directly from tissues: in situ tryptic digestions coupled with imaging mass spectrometry. Journal of Mass Spectrometry 42(2):254–262PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2010

Authors and Affiliations

  • Yuki Sugiura
    • 1
    • 2
  • Mitsutoshi Setou
    • 2
  • Daisuke Horigome
    • 3
  1. 1.Department of Bioscience and BiotechnologyTokyo Institute of TechnologyYokohamaJapan
  2. 2.Department of Molecular AnatomyHamamatsu University School of MedicineHamamatsuJapan
  3. 3.Mitsubishi Kagaku Institute of Life SciencesMachidaJapan

Personalised recommendations