Matrix Choice


In this section, the choices of matrix compound and solvent composition appropriate for tissue IMS are reviewed. As is well known, it is very important to choose an appropriate matrix for successful imaging measurement. A practical choice of matrix depends upon the type of analyte involved. Until today, in traditional MALDI-MS, a large variety of compounds has been empirically tested for their suitability in playing the role of a matrix; today, researchers can choose from a relatively small number of established “organic chemical matrices” such as sinapic acid (SA), α-cyano-4-hydroxy-cinnamic acid (CHCA), and 2,5-dihydroxybenzoic acid (DHB), and they have proven to be useful matrices for MALDI-imaging measurement. On the other hand, in MALDI-IMS, it is still necessary to develop a new matrix because of the extremely complex chemistry on the tissue surface. We also introduce some novel organic matrices and the further use of nanoparticles as an alternative to organic matrices from recent literature.


Sinapic Acid Analyte Molecule Matrix Compound MALDI Imaging Absorb Laser Energy 
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  1. 1.
    McDonnell LA, Heeren RM (2007) Imaging mass spectrometry. Mass Spectrom Rev 26:606–643PubMedCrossRefGoogle Scholar
  2. 2.
    Karas M, Bachmann D, Bahr U, et al. (1987) Matrix assisted ultraviolet-laser desorption of non-volatile compounds. Int J Mass Spectrom Ion Processes 78:53–68CrossRefGoogle Scholar
  3. 3.
    Dreisewerd K (2003) The desorption process in MALDI. Chem Rev 103:395–426PubMedCrossRefGoogle Scholar
  4. 4.
    Tanaka K, Waki H, Ido Y, et al. (1988) Protein and polymer analyses up to m/z 100,000 by laser ionization time-of flight mass spectrometry. Rapid Commun Mass Spectrom 2:151–153CrossRefGoogle Scholar
  5. 5.
    Armstrong DW, Zhang LK, He L, et al. (2001) Ionic liquids as matrixes for matrix-assisted laser desorption/ionization mass spectrometry. Anal Chem 73:3679–3686PubMedCrossRefGoogle Scholar
  6. 6.
    Lemaire R, Tabet JC, Ducoroy P, et al. (2006) Solid ionic matrixes for direct tissue analysis and MALDI imaging. Anal Chem 78:809–819PubMedCrossRefGoogle Scholar
  7. 7.
    Garrett TJ, Prieto-Conaway MC, Kovtoun V, et al. (2006) Imaging of small molecules in tissue sections with a new intermediate-pressure MALDI linear ion trap mass spectrometer. Int J Mass Spectrom 260:11Google Scholar
  8. 8.
    Cornett DS, Frappier SL, Caprioli RM (2008) MALDI-FTICR imaging mass spectrometry of drugs and metabolites in tissue. Anal Chem 80:5648–5653PubMedCrossRefGoogle Scholar
  9. 9.
    Jackson SN, Ugarov M, Egan T, et al. (2007) MALDI-ion mobility-TOFMS imaging of lipids in rat brain tissue. J Mass Spectrom 42:1093–1098PubMedCrossRefGoogle Scholar
  10. 10.
    Amantonico A, Oh JY, Sobek J, et al. (2008) Mass spectrometric method for analyzing metabolites in yeast with single cell sensitivity. Angew Chem (Int Ed) 47:5382–5385CrossRefGoogle Scholar
  11. 11.
    Burrell M, Earnshaw C, Clench M (2007) Imaging matrix assisted laser desorption ionization mass spectrometry: a technique to map plant metabolites within tissues at high spatial resolution. J Exp Bot 58:757–763PubMedCrossRefGoogle Scholar
  12. 12.
    Benabdellah F, Touboul D, Brunelle A, et al. (2009) In situ primary metabolites localization on a rat brain section by chemical mass spectrometry imaging. Anal Chem 81, 5557–5560PubMedCrossRefGoogle Scholar
  13. 13.
    Moritake S, Taira S, Sugiura Y, et al. (2009) Magnetic nanoparticle-based mass spectrometry for the detection of biomolecules in cultured cells. J Nanosci Nanotechnol 9:169–176PubMedCrossRefGoogle Scholar
  14. 14.
    Taira S, Sugiura Y, Moritake S, et al. (2008) Nanoparticle-assisted laser desorption/ionization based mass imaging with cellular resolution. Anal Chem 80:4761–4766PubMedCrossRefGoogle Scholar
  15. 15.
    McMahon JM, Short RT, McCandlish CA, et al. (1996) Identification and mapping of phos-phocholine in animal tissue by static secondary ion mass spectrometry and tandem mass spectrometry. Rapid Commun Mass Spectrom 10:335–340PubMedCrossRefGoogle Scholar
  16. 16.
    Moritake S, Taira S, Sugiura Y, et al. (2008) Magnetic nanoparticle-based mass spectrometry for the detection of biomolecules in cultured cells. J Nanosci Nanotechnol. 2009 (1):169–76.Google Scholar
  17. 17.
    Ageta H, Asai S, Sugiura Y, et al. (2008) Layer-specific sulfatide localization in rat hippocampus middle molecular layer is revealed by nanoparticle-assisted laser desorption/ionization imaging mass spectrometry. Med Mol Morphol 42:16–23 (2009)CrossRefGoogle Scholar
  18. 18.
    McLean JA, Stumpo KA, Russell DH (2005) Size-selected (2–10 nm) gold nanoparticles for matrix assisted laser desorption ionization of peptides. J Am Chem Soc 127:5304–5305PubMedCrossRefGoogle Scholar
  19. 19.
    Schwartz SA, Reyzer ML, Caprioli RM (2003) Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation. J Mass Spectrom 38:699–708PubMedCrossRefGoogle Scholar
  20. 20.
    Seeley EH, Oppenheimer SR, Mi D, et al. (2008) Enhancement of protein sensitivity for MALDI imaging mass spectrometry after chemical treatment of tissue sections. J Am Soc Mass Spectrom 19:1069–1077PubMedCrossRefGoogle Scholar
  21. 21.
    Altelaar AFM, Taban IM, McDonnell LA, et al. (2007) High-resolution MALDI imaging mass spectrometry allows localization of peptide distributions at cellular length scales in pituitary tissue sections. Int J Mass Spectrom 260:9Google Scholar
  22. 22.
    Hopfgartner G, Varesio E, Stoeckli M (2009) Matrix-assisted laser desorption/ionization mass spectrometric imaging of complete rat sections using a triple quadrupole linear ion trap. Rapid Commun Mass Spectrom 23:733–736PubMedCrossRefGoogle Scholar
  23. 23.
    Atkinson SJ, Loadman PM, Sutton C, et al. (2007) Examination of the distribution of the bioreductive drug AQ4N and its active metabolite AQ4 in solid tumours by imaging matrixassisted laser desorption/ionisation mass spectrometry. Rapid Commun Mass Spectrom 21:1271–1276PubMedCrossRefGoogle Scholar
  24. 24.
    Sugiura Y, Konishi Y, Zaima N, et al. (2009) Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry. J Lipid Res J Lipid Res 50: 1776–1788CrossRefGoogle Scholar
  25. 25.
    Astigarraga E, Barreda-Gomez G, Lombardero L, et al. (2008) Profiling and imaging of lipids on brain and liver tissue by matrix-assisted laser desorption/ ionization mass spectrometry using 2-mercaptobenzothiazole as a matrix. Anal Chem 80:9105–9114PubMedCrossRefGoogle Scholar
  26. 26.
    Wang HY, Jackson SN, Woods AS (2007) Direct MALDI-MS analysis of cardiolipin from rat organs sections. J Am Soc Mass Spectrom 18:567–577PubMedCrossRefGoogle Scholar
  27. 27.
    Suguira Y, Setou M (2009) Selective imaging of positively charged polar and nonpolar lipids by optimizing matrix solution composition. Rapid Commun Mass Spectrum 23(20): 3269–3278CrossRefGoogle Scholar

Copyright information

© Springer 2010

Authors and Affiliations

  • Yuki Sugiura
    • 1
    • 2
  • Mitsutoshi Setou
    • 2
  • Daisuke Horigome
    • 3
  1. 1.Department of Bioscience and BiotechnologyTokyo Institute of TechnologyYokohamaJapan
  2. 2.Department of Molecular AnatomyHamamatsu University School of MedicineHamamatsuJapan
  3. 3.Mitsubishi Kagaku Institute of Life SciencesMachidaJapan

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