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Matrix Choice

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Imaging Mass Spectrometry

Abstract

In this section, the choices of matrix compound and solvent composition appropriate for tissue IMS are reviewed. As is well known, it is very important to choose an appropriate matrix for successful imaging measurement. A practical choice of matrix depends upon the type of analyte involved. Until today, in traditional MALDI-MS, a large variety of compounds has been empirically tested for their suitability in playing the role of a matrix; today, researchers can choose from a relatively small number of established “organic chemical matrices” such as sinapic acid (SA), α-cyano-4-hydroxy-cinnamic acid (CHCA), and 2,5-dihydroxybenzoic acid (DHB), and they have proven to be useful matrices for MALDI-imaging measurement. On the other hand, in MALDI-IMS, it is still necessary to develop a new matrix because of the extremely complex chemistry on the tissue surface. We also introduce some novel organic matrices and the further use of nanoparticles as an alternative to organic matrices from recent literature.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan

    Yuki Sugiura

  2. Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan

    Yuki Sugiura & Mitsutoshi Setou

  3. Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo, Japan

    Daisuke Horigome

Authors
  1. Yuki Sugiura
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  2. Mitsutoshi Setou
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  3. Daisuke Horigome
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Corresponding author

Correspondence to Mitsutoshi Setou .

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Editors and Affiliations

  1. Molecular Imaging Frontier Research Center Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan

    Mitsutoshi Setou (Professor and Chairman) (Professor and Chairman)

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© 2010 Springer

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Sugiura, Y., Setou, M., Horigome, D. (2010). Matrix Choice. In: Setou, M. (eds) Imaging Mass Spectrometry. Springer, Tokyo. https://doi.org/10.1007/978-4-431-09425-8_5

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