Preparing Biological Tissue Sections for Imaging Mass Spectrometry
The process of making a thin slice section from frozen tissue blocks to the matrix application is described. Because the slice section created is served to direct mass spectrometry, there are some pivotal differences from other staining methods such as immunohistochemistry. In this chapter, the problems specific to mass spectrometry (MS) are introduced, and thereafter the ways of dealing with these problems are explained; last, the standard protocols are introduced.
KeywordsHigh Molecular Weight Protein Freeze Tissue Section Matrix Application Postmortem Degradation Good Light Transmission
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