Imaging and Molecular Identification of Biomolecules on Tissue Sections with AXIMA-QIT: Shimadzu Corporation

  • Masaru Furuta
  • Yuki Sugiura
  • Mitsutoshi Setou


In MALDI-IMS experiments, because of the complex MALDI process on the tissue surface, the ability to both visualize and identify molecules directly on the tissue surface by tandem mass spectrometry (MS n ) is essential. Here we introduce an imaging system based on a MALDI-quadrupole ion trap time-of-flight type instrument (AXIMA-QIT; Shimadzu Corporation), which is compatible with both imaging and highly sensitive MS n . In this chapter, we present the attractive features of the AXIMA-QIT as an imaging instrument and introduce the variety of application topics. The visualized biomolecules were successfully identified by MS n directly on the tissue surface, with a strong ability to isolate precursor ions. The presented IMS system with AXIMA-QIT covers a wide range of molecular imaging including phospholipids and other endogenous metabolites and also tryptic-digested proteins in various biological samples.


Myelin Basic Protein Neutral Loss Imaging Mass Spectrometry Tissue Surface Metastatic Colon Cancer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer 2010

Authors and Affiliations

  • Masaru Furuta
    • 1
  • Yuki Sugiura
    • 2
    • 3
  • Mitsutoshi Setou
    • 2
  1. 1.Applications Development Center, Analytical Applications DepartmentAnalytical and Measuring Instruments Division, Shimadzu CorporationNakagyo-kuJapan
  2. 2.Department of Bioscience and BiotechnologyTokyo Institute of TechnologyMidori-ku, YokohamaJapan
  3. 3.Department of Molecular AnatomyHamamatsu University School of MedicineHigashi-ku, HamamatsuJapan

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