The oversampling method succeeded in improving the resolution of imaging mass spectrometry (IMS) without any changes to the laser optics of their instrument. In addition, other useful tools have been also developed: Dynamic pixel imaging (patent pending) is a technique that can allow longer mass spectrometry (MS) analysis time over a defined area during a IMS experiment and it can also be used as a multiplexing tool such as dependent scan when MS to MS/MS data are simultaneously acquired. Here we report three analytical examples using these new measuring technologies.


Image Mass Spectrometry Mass Spectrometry Spectrum Laser Spot Size Desmethyl Diazepam Image Mass Spectrometry Data 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Stoeckli M, Chaurand P, Hallahan DE, Caprioli RM (2001) Imaging mass spectrometry: a new technology for the analysis of protein expression in mammalian tissues. Nat Med 7(4):493–496PubMedCrossRefGoogle Scholar
  2. 2.
    Todd PJ, Schhaaff TG, Chaurand P, Caprioli RM (2001) Organic ion imaging of biological tissue with secondary ion mass spectrometry and matrix-assisted laser desorption/ionization. J Mass Spectrom 36(4):355–369PubMedCrossRefGoogle Scholar
  3. 3.
    Chaurand P, Caprioli RM (2002) Direct profiling and imaging of peptides and proteins from mammalian cells and tissue sections by mass spectrometry. Electrophoresis 23(18):3125–3135PubMedCrossRefGoogle Scholar
  4. 4.
    Chaurand P, Schwartz SA, Caprioli RM (2002) Imaging mass spectrometry: a new tool to investigate the spatial organization of peptides and proteins in mammalian tissue sections. Curr Opin Chem Biol 6(5):676–681PubMedCrossRefGoogle Scholar
  5. 5.
    Jurchen JC, Rubakhin SS, Sweedler JV (2005) MALDI-MS imaging of features smaller than the size of the laser beam. J Am Soc Mass Spectrom 16(10):1654–1659PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2010

Authors and Affiliations

  1. 1.Applied Biosystems JapanChuo-kuJapan

Personalised recommendations