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TLC-Blot-MALDI-IMS

  • Naoko Goto-Inoue
  • Takao Taki
  • Mitsutoshi Setou

Abstract

In this chapter, we demonstrate the technique of TLC-imaging mass spectrometry consisting of thin-layer chromatography (TLC), the TLC-blot method, and imaging mass spectrometry. By the use of this method, we could separate, purify, detect, and identify very faint lipids at the same time. We could detect a lipid at a low-picomole level; this method shows higher sensitivity than the conventional staining method. This method is also suitable for molecular species differential analysis by constructing the ion image of each molecular species with fine resolution (micrometer order). Furthermore, this system is capable of quantitative analysis by the use of TLC. It needs no special equipment or technique, and thus it is easy for everyone to begin using.

Keywords

PVDF Membrane Molecular Species Image Mass Spectrometry Methyl Acetate MALDI Target Plate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 2010

Authors and Affiliations

  • Naoko Goto-Inoue
    • 1
  • Takao Taki
    • 1
  • Mitsutoshi Setou
    • 2
  1. 1.Department of Molecular AnatomyHamamatsu University School of MedicineHamamatsuJapan
  2. 2.Institute of Biomedical InnovationOtsuka Pharmaceutical Co. Ltd.Kawauchi-choJapan

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