Zusammenfassung
In diesem Kapitel dreht sich alles um die Zellkulturmedien, um deren Inhaltsstoffe und Einsatzmoglichkeiten. Aufgrund der Vielzahl der auf dem Markt erhaltlichen Produkte sollen in diesem Kapitel die Standardmedien und einige Neuentwicklungen vorgestellt werden, die dem derzeitigen Trend zur serumreduzierten bzw. serumfreien Zellkultur Rechnung tragen. Die Zellkulturzusatze und Supplemente wie Seren werden in Kapitel 8 detailliert besprochen.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Literatur
Christie A, Butler M (1999) The adaptation of BHK cells to a non-ammoniagenic glutamate-based culture medium. Biotechnol Bioeng 64(3): 298–309
Eagle H (1955) Nutrition needs of mammalian cells in tissue. Science 122: 501–504
Grace, TDC (1962) Establishment of four strains of cells from insect tissue grown in vitro. Nature 195: 788–789
Hassell T et al. (1991) Growth inhibition in animal cell culture. The effect of lactate and ammonia. Appl Biochem Biotechnol 30(1): 29–41
Jungi TW et al. (1996) Induction of nitric oxide synthase in bovine mononuclear phagocytes is differentation stage-dependent. Immunobiology 195: 385–400
Law JH, Wells MA (1989) Insects as biochemical models. J Biol Chem 264: 16335–16338
Ozisik YY et al. (1994) Trisomy 5 in long-term Cultures from bone marrow of patients with solid tumors. Cancer Genetics and Cytogenetics 78: 207–209
Sanford KK et al. (1952) The effects of ultrafiltrates and residues of horse serum and chickembryo extract on proliferation of cells in vitro. J Nat Cancer Inst 13: 121–137
Schlaeger EJ (1996) Medium design for insect cell culture. Cytotechnology 20: 57–70
Schlaeger EJ (1996) The protein hydrolysate, Primatone RL, is a cost-effective multiple growth promotor of mammalian cell culture in serum-containing and serum-free media and displays anti-apoptosis properties. J Immun Meth 194: 191–199
Schlaeger EJ et al. (1993) SF-1, a low cost culture medium for the production of recombinant proteins in baculovirus infected cells. Biotech Techn 7(3): 183–188
Weiss SA et al. (1981) Improved method for the production of insect cell cultures in large volume. In Vitro17: 495–502
Yang M & Butler M (2002) Effects of ammonia and glucosamine on the heterogeneity of erythropoietin glycoforms. Biotechnol Prog 18(1): 129–38
Yang M & Butler M (2000) Effect of ammonia on the glycosylation of human recombinant erythropoietin in culture. Biotechnol Prog 16(5): 751–759
Rights and permissions
Copyright information
© 2011 Spektrum Akademischer Verlag Heidelberg
About this chapter
Cite this chapter
Schmitz, S. (2011). Medien. In: Der Experimentator: Zellkultur. Experimentator. Spektrum Akademischer Verlag, Heidelberg. https://doi.org/10.1007/978-3-8274-2573-7_7
Download citation
DOI: https://doi.org/10.1007/978-3-8274-2573-7_7
Publisher Name: Spektrum Akademischer Verlag, Heidelberg
Print ISBN: 978-3-8274-2572-0
Online ISBN: 978-3-8274-2573-7
eBook Packages: Life Science and Basic Disciplines (German Language)