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Detektion von Protein und Nucleinsäure auf Membran

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Gentechnische Methoden

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Zusammenfassung

Beim Protein-Blotting werden Proteine auf eine Membran überführt und dort einer Nachweisreaktion unterzogen, die für ein Protein spezifisch ist oder sein soll. Die Proteine werden dazu entweder direkt auf die Membran getüpfelt (Dot-Blot) oder sie werden gelelektrophoretisch getrennt und anschließend aus dem Gel auf die Membran transferiert (Gel-Blot). Je nach Aufbau des Experiments erhält man dabei entweder Informationen über die tatsächliche Spezifität der Nachweisreaktion oder eine Aussage über Eigenschaften und Menge des Proteins. Das Blotten der Proteine auf eine Membran bietet dabei den Vorteil, dass die Membran als Trägermatrix einfacher zu handhaben ist als ein Gel. Die Proteine auf der Membranoberfläche sind immobilisiert und so auch für voluminöse Nachweisreagenzien gut zugänglich.

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Authors
  1. Steffen Bade
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  2. Niels Röckendorf
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  3. Andreas Frey
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  4. Arnd Petersen
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Monika Jansohn Sophie Rothhämel

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© 2012 Spektrum Akademischer Verlag Heidelberg

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Bade, S., Röckendorf, N., Frey, A., Petersen, A. (2012). Detektion von Protein und Nucleinsäure auf Membran. In: Jansohn, M., Rothhämel, S. (eds) Gentechnische Methoden. Spektrum Akademischer Verlag, Heidelberg. https://doi.org/10.1007/978-3-8274-2430-3_10

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