Expression analysis of salt stress responsive genes in grapevines
In Tunisia, highly tolerant autochthonous varieties represent a valuable resource for elucidating mechanisms of plant adaptation to salinity. Our aim is to determine which genes in these Tunisian grapevine varieties significantly contribute to the adaptation to increasing salinity. To characterize the complement of salt-responsive genes in Tunisian grapevines, we have constructed subtractive cDNA library from leaves of Vitis vinifera var. Razegui, a highly salt-tolerant variety. The library was screened for differentially expressed cDNAs, and positive clones were verified. The expression pattern of selected candidate cDNAs was analyzed using a range of Vitis vinifera cultivars with different degrees of phenotypic salt-stress tolerance. Expression of these cDNAs was investigated in plants after 6 and 24 h of treatment with 100 mM NaCl in hydroponic culture, and compared to plants grown under control conditions. Here, we describe the analysis of these transcripts and the putative correlation between phenotypic adaptation to salt stress and salt-induced gene expression.
KeywordsSalt Stress Cicer Arietinum Suppressive Subtractive Hybridization Grapevine Variety Suppressive Subtractive Hybridization Library
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- 2.Hachicha M, Mtimet A (1994) Les sols salés et la salinisation en Tunisie. Sols de Tunisie 15: 271–341Google Scholar
- 6.Hamrouni L, BenAbdallah F, Abdelly C, Ghorbel W (2002) Evaluation de la tolérance des vignes tunisiennes cultivées in vitro. Proceeding du XXVIIeme Congrès Mondial de la Vigne et du vin, Bratislava-Solvénie, 24–28 June 2002,102–112Google Scholar
- 7.Diatchenko L, Lau YFC, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD (1996) Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc NatlAcad Sci USA 93: 6025–6030CrossRefGoogle Scholar
- 8.Renault AS, Deloire A, Letinois I, Kraeva E, Tesniere C, Ageorges A, Redon C, Bierne J (2000) Beta-1,3-glucanase gene expression in grapevine leaves as a response to infection with Botrytis cinerea. Am J Enol Viticult 51: 81–87Google Scholar
- 10.Höfer MU, Santore UJ, Westhoff P (1992) Differential accumulation of the 10-, 16-and 23-kDa peripheral components of the water-splitting complex of photosystem II in mesophyll and bundle-sheath chloroplasts of the dicotyledonous C4 plant Flaveria trinervia (Spreng.) C. Mohr. Planta 186: 304–312CrossRefGoogle Scholar
- 11.Hoefer MU, Daldoul S, Linhard C, Ghorbel W, Krczal G, Reustle GM (2005) Isolation and characterization of salt stress-associated cDNAs from a stress-tolerant Mediterranean V. vinifera variety. In: W QiuW, LG Kovacs (eds): Proceedings of the International Grape Genomics Symposium. The International Grape Genome Program and Missouri State University, USA, 131–136Google Scholar