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Molecular analyses of a dehydration-related gene from the DREB family in durum, wheat and triticale

  • Arianna Latini
  • Maria Sperandei
  • Sandeep Sharma
  • Cristina Cantale
  • Massimo Iannetta
  • Marco Dettori
  • Karim Ammar
  • Patrizia Galeffi

Abstract

Abiotic stresses are the primary cause of crop loss worldwide. They result in average yield losses of more than 50% in major crops. The negative effects of abiotic stresses are thought to be increasing due to global climate change and the resulting erratic weather patterns. Improving crops’ ability to tolerate abiotic stresses through conventional breeding has been successful, especially in the case of wheat, as new cultivars better adapting to increasingly difficult growing conditions are being released regularly. However, as many stress-inducible genes have been identified, sequenced, characterized and insights into their functional roles in stress tolerance are being obtained, breeding programs have much to gain by exploring ways to target those stress-related genes that may be useful in their selection. If, or when, the relationship between different alleles or expression patterns of some stress-related genes is demonstrated, perfect markers for assisting breeder in selection for stress-tolerant lines can be readily obtained. Previously, we isolated and characterized the gene designated as TdDRF1 encoding for a dehydration responsive factor in durum wheat. Results obtained using plant samples of different cultivars in time-course experiments conducted in the greenhouse suggested that the expression profile of TdDRF1 upon water stress was genotype dependent. In the present paper we report results from field experiments carried at CIMMYT’s experimental fields near Obregon in Mexico, in which quantitative RT-PCR was used to monitor the expression profile of the three transcripts produced by the TdDRF1 gene under stressed (minimally irrigated) and non-stressed (fully irrigated) conditions. Tolerant and susceptible cultivars were analyzed and the results from these field experiments are compared with those from greenhouse testing.

Keywords

Durum Wheat Durum Wheat Genotype Average Yield Loss DREB Family Sive Gene Expression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag/Switzerland 2008

Authors and Affiliations

  • Arianna Latini
    • 1
  • Maria Sperandei
    • 1
  • Sandeep Sharma
    • 1
    • 2
  • Cristina Cantale
    • 1
  • Massimo Iannetta
    • 3
  • Marco Dettori
    • 4
  • Karim Ammar
    • 5
  • Patrizia Galeffi
    • 1
  1. 1.ENEA CR Casaccia BIOTEC-GENRomeItaly
  2. 2.Dept. of Genetics and Plant BreedingBanaras Hindu UniversityBanarasIndia
  3. 3.ENEA CR CasacciaBIOTEC-DESRomeItaly
  4. 4.CRAS, (Centro Regionale Agrario Sperimentale) CagliariSardiniaItaly
  5. 5.CIMMYT Centro Internacional de Mejoramiento de Maíz y TrigoEl BatánMéxico

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