P-selectin glycoprotein ligand-1 (PSGL-1)

  • Rodger P. McEver
Part of the Progress in Inflammation Research book series (PIR)


The selectins are type 1 membrane glycoproteins that have an N-terminal C-type lectin domain, followed by an epidermal growth factor (EGF)-like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail (see Chapters 2 and 3). Leukocytes express L-selectin, whereas activated platelets express P-selectin and activated endothelial cells express P-selectin and E-selectin. Each selectin mediates leukocyte rolling to vascular surfaces through Ca2+-dependent interactions of the lectin domain with cell-surface glycoconjugates. All selectins bind with low affinity to glycans with terminal components that include α2-3-linked sialic acid and a1-3-linked fucose, typified by the sialyl Lewis x (sLex) determinant (NeuAcα2-3Galβ1-4[Fucα1-3]GlcNAcβ1-R) [1, 2]. Crystal structures of sLex bound to the lectin domains of P- and E-selectin reveal a network of interactions between the fucose, a single Ca2+ ion, and several amino acids, including those that coordinate the Ca2+ [3]. This explains the Ca2+ requirement for binding. Targeted disruption of the α1-3-fucosyltransferases Fuc-TIV and Fuc-TVII in mice eliminates selectin-mediated leukocyte trafficking [4, 5]. This demonstrates that physiologically relevant selectin ligands require al-3-linked fucose. However, there is abundant evidence that selectins bind better to some glycoproteins modified with sLex-capped glycans than to others [1, 2]. This chapter focuses on the most thoroughly characterized of these glycoproteins: P-selectin glycoprotein ligand-1 (PSGL-1), which mediates important biological functions through interactions with each of the selectins.


Wall Shear Stress Leukocyte Rolling Lectin Domain Selectin Ligand Bond Lifetime 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Vestweber D, Blanks JE (1999) Mechanisms that regulate the function of the selectins and their ligands. Physiol Rev 79: 181–213PubMedGoogle Scholar
  2. 2.
    McEver RP (2002) Selectins: lectins that initiate cell adhesion under flow. Curr Opin Cell Biol 14: 581–586PubMedCrossRefGoogle Scholar
  3. 3.
    Somers WS, Tang J, Shaw GD, Camphausen RT (2000) Insights into the molecular basis of leukocyte tethering and rolling revealed by structures of Pand E-selectin bound to SLe(X) and PSGL-1. Cell 103: 467–479PubMedCrossRefGoogle Scholar
  4. 4.
    Homeister JW, Thall AD, Petrymak B, Maly P, Rogers CE, Smith PL, Kelly RJ, Gersten KM, Askari SW, Cheng GY et al (2001) The α(1,3)fucosyltransferases FucT-IV and FucT-VII exert collaborative control over selectin-dependent leukocyte recruitment and lymphocyte homing. Immunity 15: 115–126PubMedCrossRefGoogle Scholar
  5. 5.
    Smithson G, Rogers CE, Smith PL, Scheidegger EP, Petryniak B, Myers JT, Kim DSL, Homeister JW, Lowe JB (2001) Fuc-TVII is required for T helper 1 and T cytotoxic 1 lymphocyte selectin ligand expression and recruitment in inflammation, and together with Fuc-TIV regulates naive T cell trafficking to lymph nodes. J Exp Med 194: 601–614PubMedCrossRefGoogle Scholar
  6. 6.
    Moore KL, Stults NL, Diaz S, Smith DL, Cummings RD, Varki A, McEver RP (1992) Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells. J Cell Biol 118: 445–456PubMedCrossRefGoogle Scholar
  7. 7.
    Norgard KE, Moore KL, Diaz S, Stults NL, Ushiyama S, McEver RP, Cummings RD, Varki A (1993) Characterization of a specific ligand for P-selectin on myeloid cells. A minor glycoprotein with sialylated O-linked oligosaccharides. J Biol Chem 268: 12764–12774PubMedGoogle Scholar
  8. 8.
    Sako D, Chang XJ, Barone KM, Vachino G, White HM, Shaw G, Veldman GM, Bean KM, Ahern TJ, Furie B et al (1993) Expression cloning of a functional glycoprotein ligand for P-selectin. Cell 75: 1179–1186PubMedCrossRefGoogle Scholar
  9. 9.
    Veldman GM, Bean KM, Cumming DA, Eddy RL, Sait SNJ, Shows TB (1995) Genomic organization and chromosomal localization of the gene encoding human P-selectin glycoprotein ligand. J Btol Chem 270: 16470–16475CrossRefGoogle Scholar
  10. 10.
    Li F, Enckson HP, James JA, Moore KL, Cummings RD, McEver RP (1996) Visualization of P-selectin glycoprotein ligand-1 as a highly extended molecule and mapping of protein epitopes for monoclonal antibodies. J Biol Chem 271: 6342–6348PubMedCrossRefGoogle Scholar
  11. 11.
    Snapp KR, Craig R, Herron M, Nelson RD, Stoolman LM, Kansas GS (1998) Dimerization of P-selectin glycoprotein ligand-1 (PSGL-1) required for optimal recognition of P-selectin. J Cell Biol 142: 263–270PubMedCrossRefGoogle Scholar
  12. 12.
    Epperson TK, Patel KD, McEver RP, Cummings RD (2000) Noncovalent association of P-selectin glycoprotein ligand-1 and minimal determinants for binding to P-selectin. J Biol Chem 275: 7839–7853PubMedCrossRefGoogle Scholar
  13. 13.
    Yang J, Galipeau J, Kozak CA, Furie BC, Furie B (1996) Mouse P-selectin glycoprotein ligand-1: Molecular cloning, chromosomal localization, and expression of a functional P-selectin receptor. Blood 87: 4176–4186PubMedGoogle Scholar
  14. 14.
    Moore KL, Patel KD, Bruehl RE, Fugang L, Johnson DA, Lichenstein HS, Cummings RD, Bainton DF, McEver RP (1995) P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin. J Cell Biol 128: 661–671PubMedCrossRefGoogle Scholar
  15. 15.
    Laszik Z, Jansen PJ, Cummings RD, Tedder TF, McEver RP, Moore KL (1996) P-selectin glycoprotein ligand-1 is broadly expressed in cells of myeloid, lymphoid, and dendritic lineage and in some nonhematopoietic cells. Blood 88: 3010–3021PubMedGoogle Scholar
  16. 16.
    Frenette PS, Dems CV, Weiss L, Jurk K, Subbarao S, Kehrel B, Hartwig JH, Vestweber D, Wagner DD (2000) P-selectin glycoprotein ligand 1 (PSGL-1) is expressed on platelets and can mediate platelet-endothelial interactions in vivo. J Exp Med 191: 1413–1422PubMedCrossRefGoogle Scholar
  17. 17.
    Rivera-Nieves J, Burcin TL, Olson TS, Morris MA, McDuffie M, Cominelli F, Ley K (2006) Critical role of endothelial P-selectin glycoprotein ligand 1 in chronic murine ileitis. J Exp Med 203: 907–917PubMedCrossRefGoogle Scholar
  18. 18.
    da Costa Martins P, Garcia-Vallejo JJ, van Thienen JV, Fernandez-Borja M, van Gils J, Beckers C, Horrevoets AJ, Hordijk PL, Zwaginga JJ (2007) P-selectin glycoprotein ligand-1 Is expressed on endothelial cells and mediates monocyte adhesion to activated endothelium. Arterioscler Thromb Vase Biol 27: 1023–1029CrossRefGoogle Scholar
  19. 19.
    Wilkins PP, McEver RP, Cummings RD (1996) Structures of the O-glycans on P-selectin glycoprotein ligand-1 from HL-60 cells. J Biol Chem 271: 18732–18742PubMedCrossRefGoogle Scholar
  20. 20.
    Kobzdej MMA, Leppänen A, Ramachandran V, Cummings RD, McEver RP (2002) Discordant expression of selectin ligands and sialyl Lewis x-related epitopes on murine myeloid cells. Blood 100: 485–494CrossRefGoogle Scholar
  21. 21.
    Walcheck B, Moore KL, McEver RP, Kishimoto TK (1996) Neutrophil-neutrophil interactions under hydrodynamic shear stress involve L-selectin and PSGL-1: a mechanism that amplifies initial leukocyte accumulation on P-selectin in vitro. J Clin Invest 98: 1081–1087PubMedGoogle Scholar
  22. 22.
    Tu LL, Chen AJ, Delahunty MD, Moore KL, Watson SR, McEver RP, Tedder TF (1996) L-selectin binds to P-selectin glycoprotein ligand-1 on leukocytes —Interactions between the lectin, epidermal growth factor, and consensus repeat domains of the selectins determine ligand binding specificity. J Immunol 157: 3995–4004PubMedGoogle Scholar
  23. 23.
    Spertim O, Cordey AS, Monai N, Giuffre L, Schapira M (1996) P-selectin glycoprotein ligand-1 (PSGL-1) is a ligand for L-selectin on neutrophils, monocytes and CD34+ hematopoietic progenitor cells. J Cell Biol 135: 523–531CrossRefGoogle Scholar
  24. 24.
    Guyer DA, Moore KL, Lynam E, Schammel CMG, Rogelj S, McEver RP, Sklar LA (1996) P-selectin glycoprotein ligand-1 (PSGL-1) is a ligand for L-selectin in neutrophil aggregation. Blood 88: 2415–2421PubMedGoogle Scholar
  25. 25.
    Borges E, Tietz W, Steegmaier M, Moll T, Hallmann R, Hamann A, Vestweber D (1997) P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migration into inflamed skin. J Exp Med 185: 573–578PubMedCrossRefGoogle Scholar
  26. 26.
    Alon R, Fuhlbrigge RC, Finger EB, Springer TA (1996) Interactions through L-selectin between leukocytes and adherent leukocytes nucleate rolling adhesions on selectins and VCAM-1 in shear flow. J Cell Biol 135: 849–865PubMedCrossRefGoogle Scholar
  27. 27.
    Wilkins PP, Moore KL, McEver RP, Cummings RD (1995) Tyrosine sulfation of P-selectin glycoprotein ligand-1 is required for high affinity binding to P-selectin. J Biol Chem 270:22677–22680PubMedCrossRefGoogle Scholar
  28. 28.
    Sako D, Comess KM, Barone KM, Camphausen RT, Cumming DA, Shaw GD (1995) A sulfated peptide segment at the amino terminus of PSGL-1 is critical for P-selectin binding. Cell 83: 323–331PubMedCrossRefGoogle Scholar
  29. 29.
    Pouyani T, Seed B (1995) PSGL-1 recognition of P-selectin is controlled by a tyrosine sulfation consensus at the PSGL-1 amino terminus. Cell 83: 333–343PubMedCrossRefGoogle Scholar
  30. 30.
    Li F, Wilkins PP, Crawley S, Weinstein J, Cummings RD, McEver RP (1996) Posttranslational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to Pand E-selectin. J Biol Chem 271: 3255–3264PubMedCrossRefGoogle Scholar
  31. 31.
    Liu WJ, Ramachandran V, Kang J, Kishimoto TK, Cummings RD, McEver RP (1998) Identification of N-terminal residues on P-selectin glycoprotein ligand-1 required for binding to P-selectin. J Biol Chem 273: 7078–7087PubMedCrossRefGoogle Scholar
  32. 32.
    Ramachandran V, Nollert MU, Qiu H, Liu WJ, Cummings RD, Zhu C, McEver RP (1999) Tyrosine replacement in P-selectin glycoprotein ligand-1 affects distinct kinetic and mechanical properties of bonds with Pand L-selectin. Proc Natl Acad Sei USA 96: 13771–13776CrossRefGoogle Scholar
  33. 33.
    Ellies LG, Tsuboi S, Petrymak B, Lowe JB, Fukuda M, Marth JD (1998) Core 2 oligosaccharide biosynthesis distinguishes between selectin ligands essential for leukocyte homing and inflammation. Immunity 9: 881–890PubMedCrossRefGoogle Scholar
  34. 34.
    Ouyang YB, Crawley JT, Aston CE, Moore KL (2002) Reduced body weight and increased postimplantation fetal death in tyrosylprotein sulfotransferase-1-deficient mice. J Biol Chem 277: 23781–23787PubMedCrossRefGoogle Scholar
  35. 35.
    Borghei A, Ouyang YB, Westmuckett AD, Marcello MR, Landel CP, Evans JP, Moore KL (2006) Targeted disruption of tyrosylprotein sulfotransferase-2, an enzyme that catalyzes post-translational protein tyrosine O-sulfation, causes male infertility. J Biol Chem 281: 9423–9431PubMedCrossRefGoogle Scholar
  36. 36.
    Leppänen A, Mehta P, Ouyang YB, Ju T, Helm J, Moore KL, van Die I, Canfield WM, McEver RP, Cummings RD (1999) A novel glycosulfopeptide binds to P-selectin and inhibits leukocyte adhesion to P-selectin. J Biol Chem 274: 24838–24848PubMedCrossRefGoogle Scholar
  37. 37.
    Leppänen A, White SP, Helm J, McEver RP, Cummings RD (2000) Binding of glycosulfopeptides to P-selectin requires stereospecific contributions of individual tyrosine sulfate and sugar residues. J Biol Chem 275: 39569–39578PubMedCrossRefGoogle Scholar
  38. 38.
    Leppänen A, Yago T, Otto VI, McEver RP, Cummings RD (2003) Model glycosulfopeptides from P-selectin glycoprotein ligand-1 require tyrosine sulfation and a core 2-branched O-glycan to bind to L-selectin. J Biol Chem 278: 26391–26400PubMedCrossRefGoogle Scholar
  39. 39.
    Leppänen A, Penttilä L, Renkonen O, McEver RP, Cummings RD (2002) Glycosulfopeptides with O-glycans containing sialylated and polyfucosylated polylactosamine bind with low affinity to P-selectin. J Biol Chem 277: 39749–39759PubMedCrossRefGoogle Scholar
  40. 40.
    Mehta P, Cummings RD, McEver RP (1998) Affinity and kinetic analysis of P-selectin binding to P-selectin glycoprotein ligand-1. J Biol Chem 273: 32506–32513PubMedCrossRefGoogle Scholar
  41. 41.
    Croce K, Freedman SJ, Furie BC, Furie B (1998) Interaction between soluble P-selectin and soluble P-selectin glycoprotein ligand 1: Equilibrium binding analysis. Biochemistry 37:16472–16480PubMedCrossRefGoogle Scholar
  42. 42.
    Poppe L, Brown GS, Philo JS, Nikrad PV, Shah BH (1997) Conformation of sLex tetrasaccharide, free in solution and bound to E-, P-, and L-selectin. J Am Chem Soc 119: 1727–1736CrossRefGoogle Scholar
  43. 43.
    Brandley BK, Kiso M, Abbas S, Nikrad P, Srivasatava O, Foxall C, Oda Y, Hasegawa A (1993) Structure-function studies on selectin carbohydrate ligands. Modifications to fucose, sialic acid and sulphate as a sialic acid replacement. Glycobiology 3: 633–639PubMedCrossRefGoogle Scholar
  44. 44.
    Koenig A, Jain R, Vig R, Norgard-Summcht KE, Matta KL, Varki A (1997) Selectin inhibition: Synthesis and evaluation of novel sialylated, sulfated and fucosylated oligosaccharides, including the major capping group of GlyCAM-1. Glycobiology 7: 79–93PubMedCrossRefGoogle Scholar
  45. 45.
    Xia L, Ramachandran V, McDaniel JM, Nguyen KN, Cummings RD, McEver RP (2003) N-terminal residues in murine P-selectin glycoprotein ligand-1 required for binding to murine P-selectin. Blood 101: 552–559PubMedCrossRefGoogle Scholar
  46. 46.
    Moore KL, Eaton SF, Lyons DE, Lichenstein HS, Cummings RD, McEver RP (1994) The P-selectin glycoprotein ligand from human neutrophils displays sialylated, fucosylated, O-linked poly-N-acetyllactosamine. J Biol Chem 269: 23318–23327PubMedGoogle Scholar
  47. 47.
    Goetz DJ, Greif DM, Ding H, Camphausen RT, Howes S, Comess KM, Snapp KR, Kansas GS, Luscinskas FW (1997) Isolated P-selectin glycoprotein ligand-1 dynamic adhesion to Pand E-selectin. J Cell Biol 137: 509–519PubMedCrossRefGoogle Scholar
  48. 48.
    Patel KD, Moore KL, Nollert MU, McEver RP (1995) Neutrophils use both shared and distinct mechanisms to adhere to selectins under static and flow conditions. J Clin Invest 96: 1887–1896PubMedGoogle Scholar
  49. 49.
    Vachino G, Chang XJ, Veldman GM, Kumar R, Sako D, Fouser LA, Berndt MC, Cumming DA (1995) P-selectin glycoprotein ligand-1 is the major counter-receptor for Pselectin on stimulated T cells and is widely distributed in non-functional form on many lymphocytic cells. J Biol Chem 270: 21966–21974PubMedCrossRefGoogle Scholar
  50. 50.
    Ley K, Kansas GS (2004) Selectins in T-cell recruitment to non-lymphoid tissues and sites of inflammation. Nat Rev Immunol 4: 325–335PubMedCrossRefGoogle Scholar
  51. 51.
    André P, Spertini O, Guia S, Rihet P, Dignat-George F, Brailly H, Sampol J, Anderson PJ, Vivier E (2000) Modification of P-selectin glycoprotein ligand-1 with a natural killer cell-restricted sulfated lactosamine creates an alternate ligand for L-selectin. Proc Natl Acad Sci USA 97: 3400–3405PubMedCrossRefGoogle Scholar
  52. 52.
    Schäkel K, Kannagi R, Kniep B, Goto Y, Mitsuoka C, Zwirner J, Soruri A, von Kietzell M, Rieber E (2002) 6-Sulfo LacNAc, a novel carbohydrate modification of PSGL-1, defines an inflammatory type of human dendritic cells. Immunity 17: 289–301PubMedCrossRefGoogle Scholar
  53. 53.
    Rosen SD (2004) Ligands for L-selectin: homing, inflammation, and beyond. Annu Rev Immunol 22: 129–156PubMedCrossRefGoogle Scholar
  54. 54.
    Lawrence MB, Springer TA (1991) Leukocytes roll on a selectin at physiologic flow rates: Distinction from and prerequisite for adhesion through integrins. Cell 65: 859–873PubMedCrossRefGoogle Scholar
  55. 55.
    Ushiyama S, Laue TM, Moore KL, Erickson HP, McEver RP (1993) Structural and functional characterization of monomeric soluble P-selectin and comparison with membrane P-selectin. J Biol Chem 268: 15229–15237PubMedGoogle Scholar
  56. 56.
    Hattori R, Hamilton KK, Fugate RD, McEver RP, Sims PJ (1989) Stimulated secretion of endothelial von Willebrand factor is accompanied by rapid redistribution to the cell surface of the intracellular granule membrane protein GMP-140. J Biol Chem 264: 7768–7771PubMedGoogle Scholar
  57. 57.
    Yeo EL, Sheppard JAI, Feuerstein IA (1994) Role of P-selectin and leukocyte activation in polymorphonuclear cell adhesion to surface adherent activated platelets under physiologic shear conditions (an injury vessel wall model). Blood 83: 2498–2507PubMedGoogle Scholar
  58. 58.
    McEver RP, Martin MN (1984) A monoclonal antibody to a membrane glycoprotein binds only to activated platelets. J Biol Chem 259: 9799–9804PubMedGoogle Scholar
  59. 59.
    Hsu-Lin SC, Berman CL, Furie BC, August D, Furie B (1984) A platelet membrane protein expressed during platelet activation and secretion. Studies using a monoclonal antibody specific for thrombin-activated platelets. J Biol Chem 259: 9121–9126PubMedGoogle Scholar
  60. 60.
    Alon R, Hammer DA, Springer TA (1995) Lifetime of the P-selectin: carbohydrate bond and its response to tensile force in hydrodynamic flow. Nature 374: 539–542PubMedCrossRefGoogle Scholar
  61. 61.
    Ramachandran V, Yago T, Epperson TK, Kobzdej MMA, Nollert MU, Cummings RD, Zhu C, McEver RP (2001) Dimerization of a selectin and its ligand stabilizes cell rolling and enhances tether strength in shear flow. Proc Natl Acad Sci USA 98: 10166–10171PubMedCrossRefGoogle Scholar
  62. 62.
    Dembo M, Torney DC, Saxman K, Hammer D (1988) The reaction-limited kinetics of membrane-to-surface adhesion and detachment. Proc R Soc Lond B Biol Sci 234: 55–83PubMedCrossRefGoogle Scholar
  63. 63.
    Marshall BT, Long M, Piper JW, Yago T, McEver RP, Zhu C (2003) Direct observation of catch bonds involving cell-adhesion molecules. Nature 423: 190–193PubMedCrossRefGoogle Scholar
  64. 64.
    Finger EB, Puri KD, Alon R, Lawrence MB, Von Andrian UH, Springer TA (1996) Adhesion through L-selectin requires a threshold hydrodynamic shear. Nature 379: 266–269PubMedCrossRefGoogle Scholar
  65. 65.
    Lawrence MB, Kansas GS, Kunkel EJ, Ley K (1997) Threshold levels of fluid shear promote leukocyte adhesion through selectins (CD62L, P, E). / Cell Btol 136: 717–727CrossRefGoogle Scholar
  66. 66.
    Sarangapani KK, Yago T, Klopocki AG, Lawrence MB, Fieger CB, Rosen SD, McEver RP, Zhu C (2004) Low force decelerates L-selectin dissociation from P-selectin glycoprotein ligand-1 and endoglycan. J Biol Chem 279: 2291–2298PubMedCrossRefGoogle Scholar
  67. 67.
    Yago T, Wu J, Wey CD, Klopocki AG, Zhu C, McEver RP (2004) Catch bonds govern adhesion through L-selectin at threshold shear. J Cell Biol 166: 913–923PubMedCrossRefGoogle Scholar
  68. 68.
    Lou J, Yago T, Klopocki AG, Mehta P, Chen W, Zarnitsyna VI, Bovin NV, Zhu C, McEver RP (2006) Flow-enhanced adhesion regulated by a selectin interdomain hinge. J Cell Biol 174: 1107–1117PubMedCrossRefGoogle Scholar
  69. 69.
    Phan UT, Waldron TT, Springer TA (2006) Remodeling of the lectin-EGF-like domain interface in Pand L-selectin increases adhesiveness and shear resistance under hydrodynamic force. Nat Immunol 7: 883–889PubMedCrossRefGoogle Scholar
  70. 70.
    Yago T, Leppänen A, Qiu H, Marcus WD, Nollert MU, Zhu C, Cummings RD, McEver RP (2002) Distinct molecular and cellular contributions to stabilizing selectin-mediated rolling under flow. J Cell Biol 158: 787–799PubMedCrossRefGoogle Scholar
  71. 71.
    Von Andrian UH, Hasslen SR, Nelson RD, Erlandsen SL, Butcher EC (1995) A central role for microvillous receptor presentation in leukocyte adhesion under flow. Cell 82: 989–999CrossRefGoogle Scholar
  72. 72.
    Bruehl RE, Moore KL, Lorant DE, Borregaard N, Zimmerman GA, McEver RP, Bainton DF (1997) Leukocyte activation induces surface redistribution of P-selectin glycoprotein ligand-1. J Leukoc Biol 61: 489–499PubMedGoogle Scholar
  73. 73.
    Patel KD, Nollert MU, McEver RP (1995) P-selectin must extend a sufficient length from the plasma membrane to mediate rolling of neutrophils. J Cell Biol 131: 1893–1902PubMedCrossRefGoogle Scholar
  74. 74.
    Huang J, Chen J, Chesla SE, Yago T, Mehta P, McEver RP, Zhu C, Long M (2004) Quantifying the effects of molecular orientation and length on two-dimensional receptor-ligand binding kinetics. J Biol Chem 279: 44915–22923PubMedCrossRefGoogle Scholar
  75. 75.
    Shao JY, Ting-Beall HP, Hochmuth RM (1998) Static and dynamic lengths of neutrophil microvilli. Troc Natl Acad Sci USA 95: 6797–6802CrossRefGoogle Scholar
  76. 76.
    Schmidtke DW, Diamond SL (2000) Direct observation of membrane tethers formed during neutrophil attachment to platelets or P-selectin under physiological flow. J Cell Biol 149: 719–729PubMedCrossRefGoogle Scholar
  77. 77.
    Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP (2004) Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin. Proc Natl Acad Sci USA 101: 13519–13524PubMedCrossRefGoogle Scholar
  78. 78.
    Setiadi H, Sedgewick G, Erlandsen SL, McEver RP (1998) Interactions of the cytoplasmic domain of P-selectin with clathrin-coated pits enhance leukocyte adhesion under flow. J Cell Biol 142: 859–871PubMedCrossRefGoogle Scholar
  79. 79.
    Setiadi H, McEver RP (2003) Signal-dependent distribution of cell surface P-selectin in clathrin-coated pits affects leukocyte rolling under flow. J Cell Biol 163: 1385–1395PubMedCrossRefGoogle Scholar
  80. 80.
    Serrador JM, Urzainqui A, Alonso-Lebrero JL, Cabrero JR, Montoya MC, VicenteManzanares M, Yanez-Mo M, Sanchez-Madrid F (2002) A juxta-membrane amino acid sequence of P-selectin glycoprotein ligand-1 is involved in moesin binding and ezrin/ radixin/moesin-directed targeting at the trailing edge of migrating lymphocytes. Eur J Immunol 32: 1560–1566PubMedCrossRefGoogle Scholar
  81. 81.
    Snapp KR, Heitzig CE, Kansas GS (2002) Attachment of the PSGL-1 cytoplasmic domain to the actin cytoskeleton is essential for leukocyte rolling on P-selectin. Blood 99: 4494–4502PubMedCrossRefGoogle Scholar
  82. 82.
    Marcus WD, McEver RP, Zhu C (2004) Forces required to initiate membrane tether extrusion from cell surface depend on cell type but not on the surface molecule. Mech Chem Biosyst 1:245–251PubMedGoogle Scholar
  83. 83.
    Barkalow FJ, Barkalow KL, Mayadas TN (2000) Dimerization of P-selectin in platelets and endothelial cells. Blood 96: 3070–3077PubMedGoogle Scholar
  84. 84.
    Chen SQ, Springer TA (1999) An automatic braking system that stabilizes leukocyte rolling by an increase in selectin bond number with shear. J Cell Biol 144: 185–200PubMedCrossRefGoogle Scholar
  85. 85.
    Lei X, Lawrence MB, Dong C (1999) Influence of cell deformation on leukocyte rolling adhesion in shear flow. J Biomech Eng 121: 636–643PubMedGoogle Scholar
  86. 86.
    Handa K, Jacobs F, Longenecker BM, Hakoriori SI (2001) Association of MUC-1 and PSGL-1 with low-density microdomain in T-lymphocytes: a preliminary note. Biochem Biophys Res Commun 285: 788–794PubMedCrossRefGoogle Scholar
  87. 87.
    Del Conde I, Shrimpton CN, Thiagarajan P, Lopez JA (2005) Tissue-factor-bearing microvesicles arise from lipid rafts and fuse with activated platelets to initiate coagulation. Blood 106: 1604–1611PubMedCrossRefGoogle Scholar
  88. 88.
    Abbal C, Lambelet M, Bertaggia D, Gerbex C, Martinez M, Arcaro A, Schapira M, Spertini O (2006) Lipid raft adhesion receptors and Syk regulate selectin-dependent rolling under flow conditions. Blood 108: 3352–3359PubMedCrossRefGoogle Scholar
  89. 89.
    Lorant DE, Patel KD, Mclntyre TM, McEver RP, Prescott SM, Zimmerman GA (1991) Coexpression of GMP-140 and PAF by endothelium stimulated by histamine or thrombin: A juxtacrine system for adhesion and activation of neutrophils. J Cell Biol 115: 223–234PubMedCrossRefGoogle Scholar
  90. 90.
    Lorant DE, Topham MK, Whatley RE, McEver RP, Mclntyre TM, Prescott SM, Zimmerman GA (1993) Inflammatory roles of P-selectin. J Clin Invest 92: 559–570PubMedCrossRefGoogle Scholar
  91. 91.
    Elstad MR, La Pine TR, Cowley FS, McEver RP, Mclntyre TM, Prescott SM, Zimmerman GA (1995) P-selectin regulates platelet-activating factor synthesis and phagocytosis by monocytes. J Immunol 155: 2109–2122PubMedGoogle Scholar
  92. 92.
    Blanks JE, Moll T, Eytner R, Vestweber D (1998) Stimulation of P-selectin glycoprotein ligand-1 on mouse neutrophils activates ß2-integrin mediated cell attachment to ICAM1. Eur J Immunol 28: 433–443PubMedCrossRefGoogle Scholar
  93. 93.
    Hidari KI-PJ, Weyrich AS, Zimmerman GA, McEver RP (1997) Engagement of P-selectin glycoprotein ligand-1 enhances tyrosine phosphorylation and activates mitogen-activated protein kinases in human neutrophils. J Biol Chem 272: 28750–28756PubMedCrossRefGoogle Scholar
  94. 94.
    Cell A, Pellegrini G, Lorenzet R, De Blasi A, Ready N, Furie BC, Furie B (1994) P-selectin induces the expression of tissue factor on monocytes. Proc Natl Acad Sci USA 91: 8767–8771CrossRefGoogle Scholar
  95. 95.
    Damle NK, Klussman K, Dietsch MR, Mohagheghpour N, Aruffo A (1992) GMP-140 (P-selectin/CD62) binds to chronically stimulated but not resting CD4+ T lymphocytes and regulates their production of proinflammatory cytokines. Eur J Immunol 22: 1789–1793PubMedCrossRefGoogle Scholar
  96. 96.
    Haller H, Kunzendorf U, Sacherer K, Lindschau C, Walz G, Distler A, Luft FC (1997) T cell adhesion to P-selectin induces tyrosine phosphorylation of ppl25 focal adhesion kinase and other substrates. J Immunol 158: 1061–1067PubMedGoogle Scholar
  97. 97.
    Evangelista V, Manarini S, Sideri R, Rotondo S, Martelli N, Piccoli A, Totani L, Piccardoni P, Vestweber D, de Gaetano G, Cerletti C (1999) Platelet/polymorphonuclear leukocyte interaction: P-selectin triggers protein-tyrosine phosphorylation-dependent CDllb/CD18 adhesion: Role of PSGL-1 as a signaling molecule. Blood 93: 876–885PubMedGoogle Scholar
  98. 98.
    Urzainqui A, Serrador JM, Viedma F, Yanez-Mo M, Rodriguez A, Corbi AL, Alonso Lebrero JL, Luque A, Deckert M, Vazquez J, Sanchez-Madrid F (2002) ITAM-based interaction of ERM proteins with Syk mediates signaling by the leukocyte adhesion receptor PSGL-1. Immunity 17: 401–412PubMedCrossRefGoogle Scholar
  99. 99.
    Mahoney TS, Weyrich AS, Dixon DA, Mclntyre T, Prescott SM, Zimmerman GA (2001) Cell adhesion regulates gene expression at translational checkpoints in human myeloid leukocytes. Proc Natl Acad Sci USA 98: 10284–10289PubMedCrossRefGoogle Scholar
  100. 100.
    Ma YQ, Plow EF, Geng JG (2004) P-selectin binding to P-selectin glycoprotein ligand-1 induces an intermediate state of αMβ2 activation and acts cooperatively with extracellular stimuli to support maximal adhesion of human neutrophils. Blood 104: 2549–2556PubMedCrossRefGoogle Scholar
  101. 101.
    del Conde I, Nabi F, Tonda R, Thiagarajan P, Lopez JA, Kleiman NS (2005) Effect of P-selectin on phosphatidylserine exposure and surface-dependent thrombin generation on monocytes. Arterioscler Thromb Vasc Biol 25: 1065–1070PubMedCrossRefGoogle Scholar
  102. 102.
    Atarashi K, Hirata T, Matsumoto M, Kanemitsu N, Miyasaka M (2005) Rolling of Thl cells via P-selectin glycoprotein ligand-1 stimulates LFA-1-mediated cell binding to ICAM-1. J Immunol 174: 1424–1432PubMedGoogle Scholar
  103. 103.
    Woollard KJ, Kling D, Kulkarni S, Dart AM, Jackson S, Chin-Dusting J (2006) Raised plasma soluble P-selectin in peripheral arterial occlusive disease enhances leukocyte adhesion. Circ Res 98: 149–156PubMedCrossRefGoogle Scholar
  104. 104.
    Dixon DA, Tolley ND, Bemis-Standoli K, Martinez ML, Weyrich AS, Morrow JD, Prescott SM, Zimmerman GA (2006) Expression of COX-2 in platelet-monocyte interactions occurs via combinatorial regulation involving adhesion and cytokine signaling. J Clin Invest 116: 2727–2738PubMedGoogle Scholar
  105. 105.
    Smith ML, Sperandio M, Galkina EV, Ley K (2004) Autoperfused mouse flow chamber reveals synergistic neutrophil accumulation through P-selectin and E-selectin. J Leukoc Biol 76: 985–993PubMedCrossRefGoogle Scholar
  106. 106.
    Turner M, Schweighoffer E, Colucci F, Di Santo JP, Tybulewicz VL (2000) Tyrosine kinase SYK: essential functions for immunoreceptor signalling. Immunol Today 21: 148–154PubMedCrossRefGoogle Scholar
  107. 107.
    Piccardom P, Sideri R, Manarini S, Piccoli A, Martelli N, De Gaetano G, Cerletti C, Evangelista V (2001) Platelet/polymorphonuclear leukocyte adhesion: a new role for SRC kinases in Mac-1 adhesive function triggered by P-selectin. Blood 98: 108–116CrossRefGoogle Scholar
  108. 108.
    Norman KE, Moore KL, McEver RP, Ley K (1995) Leukocyte rolling in vivo is mediated by P-selectin glycoprotein ligand-1. Blood 86: 4417–4421PubMedGoogle Scholar
  109. 109.
    Borges E, Eytner R, Moll T, Steegmaier M, Campbell MA, Ley K, Mossman H, Vestweber D (1997) The P-selectin glycoprotein ligand-1 is important for recruitment of neutrophils into inflamed mouse peritoneum. Blood 90: 1934–1942PubMedGoogle Scholar
  110. 110.
    Yang J, Hirata T, Croce K, Merrill-Skoloff G, Tchernychev B, Williams E, Flaumenhaft R, Furie BC, Furie B (1999) Targeted gene disruption demonstrates that P-selectin glycoprotein ligand 1 (PSGL-1) is required for P-selectin-mediated but not E-selectin-mediated neutrophil rolling and migration. J Exp Med 190: 1769–1782PubMedCrossRefGoogle Scholar
  111. 111.
    Xia L, Sperandio M, Yago T, McDaniel JM, Cummings RD, Pearson-White S, Ley K, McEver RP (2002) P-selectin glycoprotein ligand-1-deficient mice have impaired leukocyte tethering to E-selectin under flow. J Clin Invest 109: 939–950PubMedCrossRefGoogle Scholar
  112. 112.
    Katayama Y, Hidalgo A, Chang J, Peired A, Frenette PS (2005) CD44 is a physiological E-selectin ligand on neutrophils. J Exp Med 201: 1183–1189PubMedCrossRefGoogle Scholar
  113. 113.
    Sperandio M, Smith ML, Forlow SB, Olson TS, Xia L, McEver RP, Ley K (2003) P-selectin glycoprotein ligand-1 mediates L-selectin-dependent leukocyte rolling in venules. / Exp Med 197: 1355–1363CrossRefGoogle Scholar
  114. 114.
    Hirata T, Mernll-Skoloff G, Aab M, Yang J, Furie BC, Furie B (2000) P-selectin glycoprotein ligand 1 (PSGL-1) is a physiological ligand for E-selectin in mediating T helper 1 lymphocyte migration. J Exp Med 192: 1669–1675PubMedCrossRefGoogle Scholar
  115. 115.
    Hrachovinova I, Cambien B, Hafezi-Moghadam A, Kappelmayer J, Camphausen RT, Widom A, Xia L, Kazazian HH Jr., Schaub RG, McEver RP, Wagner DD (2003) Interaction of P-selectin and PSGL-1 generates microparticles that correct hemostasis in a mouse model of hemophilia A. Nat Med 9: 1020–1025PubMedCrossRefGoogle Scholar
  116. 116.
    Falati S, Liu Q, Gross P, Mernll-Skoloff G, Chou J, Vandendnes E, Cell A, Croce K, Furie BC, Furie B (2003) Accumulation of tissue factor into developing thrombi in vivo is dependent upon microparticle P-selectin glycoprotein ligand 1 and platelet P-selectin. J Exp Med 197: 1585–1598PubMedCrossRefGoogle Scholar
  117. 117.
    André P, Hartwell D, Hrachovinová I, Saffaripour S, Wagner DD (2000) Pro-coagulant state resulting from high levels of soluble P-selectin in blood. Proc Natl Acad Sci USA 97:13835–13840PubMedCrossRefGoogle Scholar
  118. 118.
    Bodary PF, Homeister JW, Vargas FB, Wickenheiser KJ, Cudney SS, Bahrou KL, Ohman M, Rabbani AB, Eitzman DT (2007) Generation of soluble Pand E-selectins in vivo is dependent on expression of P-selectin glycoprotein ligand-1. J Thromb Haemost 5: 599–603PubMedCrossRefGoogle Scholar
  119. 119.
    Frenette PS, Subbarao S, Mazo IB, Von Andrian UH, Wagner DD (1998) Endothelial selectins and vascular cell adhesion molecule-1 promote hematopoietic progenitor homing to bone marrow. Proc Natl Acad Sci USA 95: 14423–14428PubMedCrossRefGoogle Scholar
  120. 120.
    Katayama Y, Hidalgo A, Furie BC, Vestweber D, Furie B, Frenette PS (2003) PSGL-1 participates in E-selectin-mediated progenitor homing to bone marrow: evidence for cooperation between E-selectin ligands and α4 integrin. Blood 102: 2060–2067PubMedCrossRefGoogle Scholar
  121. 121.
    Hidalgo A, Weiss LA, Frenette PS (2002) Functional selectin ligands mediating human CD34+ cell interactions with bone marrow endothelium are enhanced postnatally. J Clin Invest 110: 559–569PubMedCrossRefGoogle Scholar
  122. 122.
    Xia L, McDaniel JM, Yago T, Doeden A, McEver RP (2004) Surface fucosylation of human cord blood cells augments binding to P-selectin and E-selectin and enhances engraftment in bone marrow. Blood 104: 3091–3096PubMedCrossRefGoogle Scholar
  123. 123.
    Hidalgo A, Frenette PS (2005) Enforced fucosylation of neonatal CD34+ cells generates selectin ligands that enhance the initial interactions with microvessels but not homing to bone marrow. Blood 105: 567–575PubMedCrossRefGoogle Scholar
  124. 124.
    Rossi FM, Corbel SY, Merzaban JS, Carlow DA, Gossens K, Duenas J, So L, Yi L, Ziltener HJ (2005) Recruitment of adult thymic progenitors is regulated by P-selectin and its ligand PSGL-1. Nat Immunol 6: 626–634PubMedCrossRefGoogle Scholar
  125. 125.
    Hirata T, Furukawa Y, Yang BG, Hieshima K, Fukuda M, Kannagi R, Yoshie O, Miyasaka M (2004) Human P-selectin glycoprotein ligand-1 (PSGL-1) interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus. / Biol Chem 279: 51775–51782CrossRefGoogle Scholar
  126. 126.
    Pendu R, Terraube V, Christophe OD, Gahmberg CG, de Groot PG, Lenting PJ, Denis CV (2006) P-selectin glycoprotein ligand 1 and β2-integrins cooperate in the adhesion of leukocytes to von Willebrand factor. Blood 108: 3746–3752PubMedCrossRefGoogle Scholar
  127. 127.
    Zheng PS, Vais D, Lapierre D, Liang YY, Lee V, Yang BL, Yang BB (2004) PG-M/versican binds to P-selectin glycoprotein ligand-1 and mediates leukocyte aggregation. / Cell Sci 117: 5887–5895CrossRefGoogle Scholar
  128. 128.
    Herron MJ, Nelson CM, Larson J, Snapp KR, Kansas GS, Goodman JL (2000) Intracellular parasitism by the human granulocytic ehrlichiosis bacterium through the P-selectin ligand, PSGL-1. Science 288: 1653–1656PubMedCrossRefGoogle Scholar
  129. 129.
    Yago T, Leppanen A, Carlyon JA, Akkoyunlu M, Karmakar S, Fikrig E, Cummings RD, McEver RP (2003) Structurally distinct requirements for binding of P-selectin glycoprotein ligand-1 and sialyl Lewis x to Anaplasma phagocytophilum and P-selectin. J Biol Chem 278: 37987–37997PubMedCrossRefGoogle Scholar
  130. 130.
    Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ (2007) Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling. Blood 109: 2936–2943PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag Basel/Switzerland 2007

Authors and Affiliations

  • Rodger P. McEver
    • 1
  1. 1.Cardiovascular Biology Research ProgramOklahoma Medical Research FoundationOklahoma CityUSA

Personalised recommendations