P-selectin glycoprotein ligand-1 (PSGL-1)

  • Rodger P. McEver
Part of the Progress in Inflammation Research book series (PIR)


The selectins are type 1 membrane glycoproteins that have an N-terminal C-type lectin domain, followed by an epidermal growth factor (EGF)-like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail (see Chapters 2 and 3). Leukocytes express L-selectin, whereas activated platelets express P-selectin and activated endothelial cells express P-selectin and E-selectin. Each selectin mediates leukocyte rolling to vascular surfaces through Ca2+-dependent interactions of the lectin domain with cell-surface glycoconjugates. All selectins bind with low affinity to glycans with terminal components that include α2-3-linked sialic acid and a1-3-linked fucose, typified by the sialyl Lewis x (sLex) determinant (NeuAcα2-3Galβ1-4[Fucα1-3]GlcNAcβ1-R) [1, 2]. Crystal structures of sLex bound to the lectin domains of P- and E-selectin reveal a network of interactions between the fucose, a single Ca2+ ion, and several amino acids, including those that coordinate the Ca2+ [3]. This explains the Ca2+ requirement for binding. Targeted disruption of the α1-3-fucosyltransferases Fuc-TIV and Fuc-TVII in mice eliminates selectin-mediated leukocyte trafficking [4, 5]. This demonstrates that physiologically relevant selectin ligands require al-3-linked fucose. However, there is abundant evidence that selectins bind better to some glycoproteins modified with sLex-capped glycans than to others [1, 2]. This chapter focuses on the most thoroughly characterized of these glycoproteins: P-selectin glycoprotein ligand-1 (PSGL-1), which mediates important biological functions through interactions with each of the selectins.


Wall Shear Stress Leukocyte Rolling Lectin Domain Selectin Ligand Bond Lifetime 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Birkhäuser Verlag Basel/Switzerland 2007

Authors and Affiliations

  • Rodger P. McEver
    • 1
  1. 1.Cardiovascular Biology Research ProgramOklahoma Medical Research FoundationOklahoma CityUSA

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