Methods, applications and concepts of metabolite profiling: Secondary metabolism

  • Lloyd W. Sumner
  • David V. Huhman
  • Ewa Urbanczyk-Wochniak
  • Zhentian Lei
Part of the Experientia Supplementum book series (EXS, volume 97)


Plants manufacture a vast array of secondary metabolites/natural products for protection against biotic or abiotic environmental challenges. These compounds provide increased fitness due to their antimicrobial, anti-herbivory, and/or alleopathic activities. Secondary metabolites also serve fundamental roles as key signaling compounds in mutualistic interactions and plant development. Metabolic profiling and integrated functional genomics are advancing the understanding of these intriguing biosynthetic pathways and the response of these pathways to environmental challenges. This chapter provides an overview of the basic methods, select applications, and future directions of metabolic profiling of secondary metabolism. The emphasis of the application section includes the combination of primary and secondary metabolic profiling. The future directions section describes the need for increased chromatographic and mass resolution, as well as the inevitable need and benefit of spatially and temporally resolved metabolic profiling.


High Performance Liquid Chromatography Secondary Metabolism Peak Capacity Aristolochic Acid Triterpene Saponin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Birkhäuser Verlag/Switzerland 2007

Authors and Affiliations

  • Lloyd W. Sumner
    • 1
  • David V. Huhman
    • 1
  • Ewa Urbanczyk-Wochniak
    • 1
  • Zhentian Lei
    • 1
  1. 1.Plant Biology DivisionThe Samuel Roberts Noble FoundationArdmoreUSA

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