Summary
The aim was to study if neural grafts placed in brain infarcts could improve functional recovery.
The middle cerebral artery was occluded (MCAO) in 19 spontaneously hypertensive rats. Nine rats were sham operated. Twelve to 16 days after the ischemic insult, 9 of the MCAO rats received transplants of dissociated fetal neocortical tissue (MCAO-T) and 1,3 and 6 months after transplantation surgery, the rats were behaviorally evaluated by a test for forelimb function. Infarct and transplant sizes were measured morphometrically.
The remaining volume of the infarcted hemisphere was 66 ± 7% (mean ± SD) in the MCAO group and 71 ± 9% in the MCAO-T group of the non-operated hemisphere. All grafted rats had surviving transplants. Contralateral to the lesion, paw-reaching was highly impaired in both infarcted groups compared with sham-operated controls with no significant difference between MCAO and MCAOT. The lesion size correlated significantly with contralateral pawreach performance at all test periods.
We conclude that neocortical grafts did not alleviate the impaired forepaw function.
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References
Björklund A, Stenevi U, Schmidt RH, Dunnett SB, Gage FH (1983) Intracerebral grafting of neuronal cell suspensions I. Introduction and general methods of preparation. Acta Physiol Scand 522: 1–7
Brundin P, Strecker RE (1991) Preparation and intracerebral grafting of dissociated fetal brain tissue in rats. In: Conn PM (ed) Methods in neurosciences, Vol 7. Lesions and transplantation. Academic Press, San Diego, pp 305–326
Coyle P (1982) Middle cerebral artery occlusion in the young rat. Stroke 13: 855–859
Eagleson KL, Cunningham TJ, Haun F (1992) Rescue of both rapidly and slowly degenerating neurons in the dorsal lateral geniculate nucleus of adult rats by a cortically derived neuron survival factor. Exp Neurol 116: 156–162
Grabowski M, Brundin P, Johansson BB (1992) Fetal neo-cortical grafts implanted in adult hypertensive rats with cortical infarcts following a middle cerebral artery occlusion: ingrowth of afferent fibers from the host brain. Exp Neurol 116: 105–121
Grabowski M, Christofferson RH, Brundin P, Johansson BB (1992) Vascularization of fetal neocortical grafts implanted in brain infarcts in spontaneously hypertensive rats. Neuroscience 51: 673–682
Grabowski M, Brundin P, Johansson BB (1993) Paw-reaching, sensorimotor, and rotational behavior after brain infarction in rats. Stroke 24: 889–895
Grabowski M, Brundin P, Johansson BB (1993) Functional integration of cortical grafts placed in brain infarcts of rats. Ann Neurol 34: 362–368
Grabowski M, Johansson BB, Brundin P (1994) Survival of fetal neocortical grafts implanted in brain infarcts of adult rats: the influence of post-lesion time and the age of donor tissue. Exp Neurol 127: 126–136
Grabowski M, Johansson BB, Brundin P (1995) Neocortical grafts placed in the infarcted brain of adult rats: few or no efferent fibers grow from transplant to host. Exp Neurol 134: 273–276
Grabowski M, Sorensen JC, Mattsson B, Zimmer J, Johansson BB (1995) Influence of an enriched environment and cortical grafting in brain infarcts of adult rats. Exp Neurol 133: 96–102
Haun F, Cunningham TJ (1984) Cortical transplants reveal CNS trophic interactions in situ. Dev Brain Res 15: 290–294
Hedreen JC, Bacon Si, Price DL (1985) A modified histochemical technique to visualize acetylcholinesterase-containing axons. J Histochem Cytochem 30: 134–140
Montoya CP, Campbell-Hope LJ, Pemberton KD, Dunnett SB (1991) The “staircase test”: a measure of independent forelimb reaching and grasping abilities in rats. J Neurosci Meth 36: 219–228
Paxinos G, Watson C (1982) The rat brain in stereotaxic coordinates. Academic Press, Sydney
Sharp FR, Gonzalez MF (1986) Fetal cortical transplants ameliorate thalamic atrophy ipsilateral to neonatal frontal cortex lesions. Neurosci Lett 71: 247–251
Sofroniew MV, Isacson O, Björklund A (1986) Cortical grafts prevent atrophy of cholinergic basal nucleus neurons induced by excitotoxic cortical damage. Brain Res 378: 409–415
Sorensen JC, Zimmer J, Castro AJ (1989) Fetal cortical transplants reduce the thalamic atrophy induced by frontal cortical lesions in newborn rats. Neurosci Lett 98: 33–38
Tamura A, Graham DI, McCulloch J, Teasdale GM (1981) Focal cerebral ischaemia in the rat: 1. Description of technique and early neuropathological consequences following middle cerebral artery occlusion. J Cereb Blood Flow Metab 1: 53–60
Whishaw IQ, O’Connor WT, Dunnett SB (1986) The contributions of motor cortex, nigrostriatal dopamine and caudateputamen to skilled forelimb use in the rat. Brain 109: 805–843
Yamada K, Kinoshita A, Kohmura E, Sakaguchi T, Taguchi J, Kataoka K, Hayakawa T (1991) Basic fibroblast growth factor prevents thalamic degeneration after cortical infarction. J Cereb Blood Flow Metab 11: 472–478
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Grabowski, M., Johansson, B.B., Brundin, P. (1996). Fetal Neocortical Grafts Placed in Brain Infarcts Do Not Improve Paw-Reaching Deficits in Adult Spontaneously Hypertensive Rats. In: Baethmann, A., Kempski, O.S., Plesnila, N., Staub, F. (eds) Mechanisms of Secondary Brain Damage in Cerebral Ischemia and Trauma. Acta Neurochirurgica, vol 66. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9465-2_12
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DOI: https://doi.org/10.1007/978-3-7091-9465-2_12
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