Summary
Progesterone receptors (PgR) are detectable in about 60–70% of tissue specimens of human meningiomas. Despite these data, PgR are hardly to be found in monolayer tissue culture of meningiomas. Aim of this study was to elucidate whether PgR might be preserved in tumor fragment spheroids of meningiomas maintained in organ culture since the morphological appearance of the original tumor is preserved by this culture technique.
Aliquots of meningioma specimens of 25 patients (17 females) were snap frozen in liquid nitrogen immediately after removal. Additionally, monolayer tissue cultures of the same specimen were obtained as primary culture and passage #3. Tumor fragment spheroids were kept on medium-agar with liquid medium overlay and harvested after 1 and 3 weeks in culture. PgR were detected by immunohistochemistry using a rat monoclonal antibody.
18/25 meningioma tissue specimens were positive for PgR. In 8 out of 15 PgR-positive tumors which formed spheroids we could detect PgR in fragment spheroids after 1 and 3 weeks in culture. In contrast, none of the monolayers depicted PgR. PgR is preserved in a considerable amount of tumor fragment spheroids of PgR-positive meningiomas. They remain detectable after 3 weeks of culture whereas monolayer tissue cultures are PgR-negative. Thus, tumor fragment spheroids seem to be a suitable tool to investigate progesterone/antiprogesterone effects in vitro.
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References
Adams EF, Schrell UMH, Fahlbusch R, Thierauf P (1990) Hormonal dependency of cerebral meningiomas. Part 2: In vitro effect of steroids, bromocriptine, and epidermal growth factor on growth of meningiomas. J Neurosurg 73: 750–755
Blankenstein MA, Blaauw G, Lamberts SWJ, Mulder E (1983) Presence of progesterone receptors and absence of oestrogen receptors in human intracranial meningioma cytosols. Eur J Cancer Clin Oncol 19: 363–370
Black P (1993) Meningiomas. Neurosurgery 32: 643–657
Bjerkvig R, Tonnesen A, Laerum OD, Backlund EC) (1990) Multicellular tumor spheroids from human gliomas maintained in organ culture. J Neurosurg 72: 463–375
Bjerkvig R, Hostmark J, Pedersen PH, Laerum OD (1992) Tumor spheroids from biopsy specimens. In: Bjerkvig R (ed) Spheroid culture in cancer research. CRC, Boca Raton, pp 41–56
Carrol RS, Glowacka D, Dashner K, Black PM (1993) Progesterone receptor expression in meningiomas. Cancer Res 53: 1312–1316
Grunberg SM, Weiss MH, Spitz IM, Ahmadi J, Sadun A, Russel CA, Lucci L, Stevenson LL (1991) Treatment of un-resectable meningiomas with the antiprogesterone agent mifepristone. J Neurosurg 74: 861–866
Halper J, Colvard DS, Scheithauer BW, Jiang NS, Press MF, Graham ML, Riehl E, Laws ER, Spelsberg TC (1989) Estrogen and progesterone receptors in meningiomas: comparison of nuclear binding, dextran-coated charcoal, and immunoperoxidase staining assays. Neurosurgery 25: 546–533
Jay JJ, MacLaughlin DT, Riley KR, Martuza RL (1985) Modulation of meningioma cell growth by sex steroid hormones in vitro. J Neurosurg 62: 757–762
Markwalder TM, Zava DT (1986) Sex steroid hormones and meningioma cell growth. J Neurosurg 64: 341–342
Martuza RL, Miller DC, MacLauglin DT (1985) Estrogen and progestin binding by cytosolic and nuclear fractions of human meningiomas. J Neurosurg 62: 750–756
Matsuda Y, Kawamoto K, Kiya K, Kurisu K, Sugiyama K, Uozumi T (1994) Antitumor effects of antiprogesterones on human meningioma cells in vitro and in vivo. J Neurosurg 80: 527–534
Olsen JJ, Beck DW, Schlechte J, Loh PM (1986) Hormonal manipulation of meningiomas in vitro. J Neurosurg 65: 99–107
Paulus W, Huettner C, Tonn JC (1994) Collagens, integrins and the mesenchymal drift in glioblastomas: a comparison of biopsy specimens, spheroid and early monolayer cultures. Int J Cancer 58: 841–846
Schrell UMH, Adams EF, Fahlbusch R, Greb R, Jirikowski G, Prior R, Ramalho-Ortigao FJ (1990) Hormonal dependency of cerebral meningiomas. Part 1: female sex steroid receptors and their significance as specific markers for adjuvant medical therapy. J Neurosurg 73: 743–749
Waelti ER, Markwalder TM (1989) Endocrine manipulation of meningiomas with medoxiprogesterone acetate: effect of MPA on growth of meningioma cells in monolayer tissue culture. Surg Neurol 31: 96–100
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© 1996 Springer-Verlag
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Tonn, J.C., Ott, M.M., Paulus, W., Meixensberger, J., Roosen, K. (1996). Progesterone Receptors in Tumor Fragment Spheroids of Human Meningiomas. In: Fahlbusch, R., Bock, W.J., Brock, M., Buchfelder, M., Klinger, M. (eds) Modern Neurosurgery of Meningiomas and Pituitary Adenomas. Acta Neurochirurgica, vol 65. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9450-8_29
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DOI: https://doi.org/10.1007/978-3-7091-9450-8_29
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