An aggregate brain cell culture model for studying neuronal degeneration and regeneration
Rotation-mediated aggregating cell cultures from fetal rat telencephalons containing glial and neuronal cells mature in a fashion comparable to that known to occur in brain in vivo. Large aggregates of 300–500 µM diameters can now reproducibly be cultivated and maintained for more than 40 days in a well defined serum free medium. Validity of the use of such cultures for in vitro studies of various physiological, pharmacological and toxicological phenomenon has already been demonstrated. In this communication some observations suggesting the usefulness of such cultures for pharmacological studies clarifying the possible effects of drugs and other agents on excitatory amino acid induced pathological processes will be presented. The advantages and limitations of the use of aggregated brain cell culture based models for the development of agents potentially useful for the treatment of aging and dementia will also be discussed.
KeywordsGlutamic Acid Excitatory Amino Acid Kainic Acid Excitatory Amino Acid Receptor Glycine Concentration
Unable to display preview. Download preview PDF.
- Ehrenpreis S, Chiesa A, Bigo-Gullino M, Patrick P (1967) Correlation between acetylcholin (ACH) potentiation and cholinesterase (CHE) inhibition by diisopropylfluorophosphate ( DFP) using a simplified radiometric assay for the enzyme. Fed Proc 26: 296Google Scholar
- Garber BB (1977) Cell aggregation and recognition in the self-assembly of brain tissue. In: Fedoroff S, Herz L (eds) Cell, tissue and organ cultures in neurobiology. Academic Press, New York, pp 515–537Google Scholar
- Halks-Miller M, Toll L, Davies MF (1989) Excitotoxins and antioxidants in reaggregate cultures of developing rat brain. Neurosci Abstr 15: 764Google Scholar
- Matthieu JM, Honegger P, Favrod P, Poduslo JF, Constatino-Ceccarini E, Krstic R (1980) Myelination and demyelination in aggregating cultures of rat brain cells. In: Giacobinin E, et al (eds) Tissue culture in neurobiology. Raven Press, New York, pp 441–459Google Scholar
- Muscona AA (1960) Patterns and mechanisms of tissue reconstruction from dissociated cells. In: Rudnick D (ed) Developing cell systems and their control. Ronald Press, New York, pp 45–70Google Scholar
- Muscona AA (1965) Recombination of dissociated cells and the development of cell aggregates. In: Willmer E (ed) Cells and tissue culture, vol 1. Academic Press, New York, pp 489–529Google Scholar
- Nöldner M, Chatterjee SS (1993) Excitatory amino acid induced toxicity in the aggregate cell cultures from fetal rat brain. Naunyn Schmiedebergs Arch Pharmacol [Suppl] 347: R133Google Scholar
- Seeds NW (1973) Differentiation of aggregating brain cell cultures. In: Sato G (ed) Tissue culture of the nervous system. Plenum, New York, pp 35–53Google Scholar