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Age-related alterations by chronic intermittent hypoxia on cerebral synaptosomal ATPase activities

  • G. Benzi
  • A. Gorini
  • R. Arnaboldi
  • B. Ghigini
  • R. F. Villa
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 44)

Summary

The age-related alterations in the plasticity of synaptic energy-requiring ATPases [Na+,K+-ATPase, low- and high-affinity Ca2+-ATPase, Mg2+-ATPase, and Ca2+,Mg2+-ATPase] were assayed in synaptosomes and synaptosomal subtractions [namely, synaptosomal plasma membranes and synaptic vesicles] in the cerebral cortex from 3- and 24-month-old normoxic rats and rats subjected to either mild or severe chronic (four weeks) intermittent normobaric hypoxia.

With the exception of the high-affinity Ca2+-ATPase, aging induced a decrease in the ATPase activities from normoxic rats. The adaptation to mild hypoxia was characterized by an increase in the activity of Mg2+-ATPase in 3-month-old rats, concomitant with a decrease in the activities of: (i) Na+,K+-ATPase and high-affinity Ca2+-ATPase in both 3- and 24-month-old rats, and (ii) Ca2+,Mg2+-ATPase in 3-month-old ones.

The adaptation to chronic intermittent severe hypoxia was characterized by a decrease in the activities of: (i) Na+,K+-ATPase, Ca2+,Mg2+-ATPase and high-affinity Ca2+-ATPase in both 3- and 24-month-old rats, and (ii) low-affinity Ca2+-ATPase only in 24-month-old ones. The effect on Mg2+-ATPase activity was characterized by a decrease in the activity of the enzymatic form located in the synaptic plasma membranes [involved in ATP hydrolysis to adenosine production], concomitant with an increase in the activity of the form located in the synaptic vesicles [involved in the turnover of transmitters, e.g., glutamate].

Keywords

ATPase Activity Synaptic Vesicle Adenylate Kinase Synaptic Membrane Chronic Intermittent Hypoxia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • G. Benzi
    • 1
  • A. Gorini
    • 1
  • R. Arnaboldi
    • 1
  • B. Ghigini
    • 1
  • R. F. Villa
    • 1
  1. 1.Institute of PharmacologyState University of PaviaPaviaItaly

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