Age-related alterations by chronic intermittent hypoxia on cerebral synaptosomal ATPase activities

  • G. Benzi
  • A. Gorini
  • R. Arnaboldi
  • B. Ghigini
  • R. F. Villa
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 44)


The age-related alterations in the plasticity of synaptic energy-requiring ATPases [Na+,K+-ATPase, low- and high-affinity Ca2+-ATPase, Mg2+-ATPase, and Ca2+,Mg2+-ATPase] were assayed in synaptosomes and synaptosomal subtractions [namely, synaptosomal plasma membranes and synaptic vesicles] in the cerebral cortex from 3- and 24-month-old normoxic rats and rats subjected to either mild or severe chronic (four weeks) intermittent normobaric hypoxia.

With the exception of the high-affinity Ca2+-ATPase, aging induced a decrease in the ATPase activities from normoxic rats. The adaptation to mild hypoxia was characterized by an increase in the activity of Mg2+-ATPase in 3-month-old rats, concomitant with a decrease in the activities of: (i) Na+,K+-ATPase and high-affinity Ca2+-ATPase in both 3- and 24-month-old rats, and (ii) Ca2+,Mg2+-ATPase in 3-month-old ones.

The adaptation to chronic intermittent severe hypoxia was characterized by a decrease in the activities of: (i) Na+,K+-ATPase, Ca2+,Mg2+-ATPase and high-affinity Ca2+-ATPase in both 3- and 24-month-old rats, and (ii) low-affinity Ca2+-ATPase only in 24-month-old ones. The effect on Mg2+-ATPase activity was characterized by a decrease in the activity of the enzymatic form located in the synaptic plasma membranes [involved in ATP hydrolysis to adenosine production], concomitant with an increase in the activity of the form located in the synaptic vesicles [involved in the turnover of transmitters, e.g., glutamate].


ATPase Activity Synaptic Vesicle Adenylate Kinase Synaptic Membrane Chronic Intermittent Hypoxia 
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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • G. Benzi
    • 1
  • A. Gorini
    • 1
  • R. Arnaboldi
    • 1
  • B. Ghigini
    • 1
  • R. F. Villa
    • 1
  1. 1.Institute of PharmacologyState University of PaviaPaviaItaly

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