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Cell and Animal Models in Aging and Dementia Research pp 1–20Cite as

Primary cultures of neurons for testing neuroprotective drug effects

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Part of the book series: Journal of Neural Transmission ((NEURAL SUPPL,volume 44))

Summary

Primary cultures of neurons are widely used for the investigation of pathomechanisms of neuronal damage und for the evaluation of neuroprotective drug effects. The present paper gives a short survey of frequently used primary neuronal culture systems and of experimental measures for the induction of defined neuronal damage with particular respect to the pathomechanisms of cerebral ischemia. Neuroprotective durg effects as achieved under these conditions are reviewed, and the neuroprotective effects of glutamate antagonists, radical scavengers, and neural growth factors are discussed in some more detail.

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Abbreviations

AMPA:

α-Amino-3-hydroxy-5-methylisoxazole-4-propionate

APV:

2-Amino-5-phosphonovaleric acid

CNQX:

6-Cyano-7-nitroquinoxaline-2,3-dione

DNQX:

6,7-Dinitroquinoxaline-2,3-dione

LDH:

Lactate dehydrogenase

NBQX:

2,3-Dihydro-6-nitro-7-sulphamoyl-benzo(f)quinoxaline

NMDA:

N-Methyl-D-aspartate

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Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie, Philipps-Universität, Ketzerbach 63, D-35032, Marburg, Federal Republic of Germany

    H. Oberpichler-Schwenk & Prof. Dr. Dr. J. Krieglstein

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  1. H. Oberpichler-Schwenk
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  2. Prof. Dr. Dr. J. Krieglstein
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Editors and Affiliations

  1. Institute for Pathochemistry and General Neurochemistry, University of Heidelberg, Heidelberg, Federal Republic of Germany

    S. Hoyer , D. Müller  & K. Plaschke ,  & 

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Oberpichler-Schwenk, H., Krieglstein, J. (1994). Primary cultures of neurons for testing neuroprotective drug effects. In: Hoyer, S., Müller, D., Plaschke, K. (eds) Cell and Animal Models in Aging and Dementia Research. Journal of Neural Transmission, vol 44. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9350-1_1

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