Crystallographic and cryo EM analysis of virion-receptor interactions

  • M. G. Rossmann
  • N. H. Olson
  • P. R. Kolatkar
  • M. A. Oliveira
  • R. H. Cheng
  • J. M. Greve
  • A. McClelland
  • T. S. Baker
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 9)


Cryoelectron microscopy has been used to determine the first structure of a virus when complexed with its glycoprotein cellular receptor. Human rhinovirus 16 (HRV16) complexed with the two amino-terminal, immunoglobulin-like domains of the intercellular adhesion molecule-1 (ICAM-1) shows that ICAM-1 binds into the 12 Å deep “canyon” on the surface of the virus. This is consistent with the prediction that the viral receptor attachment site lies in a cavity inaccessible to the host’s antibodies. The atomic structures of HRV14 and CD4, homologous to HRV16 and ICAM-1, showed excellent correspondence with observed density, thus establishing the virus-receptor interactions.


Cryoelectron Microscopy Human Rhinovirus Empty Capsid Canyon Floor Poliovirus Receptor 


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • M. G. Rossmann
    • 1
  • N. H. Olson
    • 1
  • P. R. Kolatkar
    • 1
  • M. A. Oliveira
    • 1
  • R. H. Cheng
    • 1
  • J. M. Greve
    • 2
  • A. McClelland
    • 2
  • T. S. Baker
    • 1
  1. 1.Department of Biological SciencesPurdue UniversityWest LafayetteUSA
  2. 2.Institute for Molecular Biologicals, Miles Inc.West HavenUSA

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