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Comparative studies of T = 3 and T = 4 icosahedral RNA insect viruses

  • J. E. Johnson
  • S. Munshi
  • L. Liljas
  • D. Agrawal
  • N. H. Olson
  • V. Reddy
  • A. Fisher
  • B. McKinney
  • T. Schmidt
  • T. S. Baker
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 9)

Summary

Crystallographic and molecular biological studies of T = 3 nodaviruses (180 identical subunits in the particle) and T = 4 tetraviruses (240 identical subunits in the particle) have revealed similarity in both the architecture of the particles and the strategy for maturation. The comparative studies provide a novel opportunity to examine an apparent evolution of particle size, from smaller (T = 3) to larger (T = 4), with both particles based on similar subunits. The BBV and FHV nodavirus structures are refined at 2.8 Å and 3 Å respectively, while the NωV structure is at 6 Å resolution. Nevertheless, the detailed comparisons of the noda and tetravirus X-ray electron density maps show that the same type of switching in subunit twofold contacts is used in the T = 3 and T = 4 capsids, although differences must exist between quasi and icosahedral threefold contacts in the T = 4 particle that have not yet been detected. The analyses of primary and tertiary structures of noda and tetraviruses show that NωV subunits undergo a post assembly cleavage like that observed in nodaviruses and that the cleaved 76 C-terminal residues remain associated with the particle.

Keywords

Coat Protein Capsid Protein Sindbis Virus Icosahedral Symmetry Tomato Bushy Stunt Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • J. E. Johnson
    • 1
  • S. Munshi
    • 1
  • L. Liljas
    • 1
  • D. Agrawal
    • 1
  • N. H. Olson
    • 1
  • V. Reddy
    • 1
  • A. Fisher
    • 1
  • B. McKinney
    • 1
  • T. Schmidt
    • 1
  • T. S. Baker
    • 1
  1. 1.Department of Biological SciencesPurdue UniversityWest LafayetteUSA

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