Mouse hepatitis virus receptors: more than a single carcinoembryonic antigen

  • K. Yokomori
  • M. M. C. Lai
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 9)


Mouse hepatitis virus (MHV), a murine coronavirus, has been shown to utilize carcinoembryonic antigen (CEA) as the receptor. We have demonstrated that MHV can utilize a different isoform of CEA, which is an alternatively spliced gene product that is expressed in different tissues, as a receptor. Furthermore, the CEA molecules from a resistant mouse strain (SJL) have different sequences and yet serve as functional viral receptors. Thus, MHV can use more than a single type of CEA molecule as the receptor. We have also shown that some mouse cell lines express functional CEA molecules and yet are resistant to infections by certain MHV strains. Biochemical studies of the infected cells indicate that MHV infections in these cell lines are blocked at the steps of virus entry. We conclude that MHV entry requires additional cellular factors other than CEA, the viral receptor. The significance of viral receptors and the additional cellular factors in regulating viral tropism is discussed.


Virus Entry Resistant Cell Line Mouse Cell Line Mouse Hepatitis Virus Target Cell Specificity 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Barthold SW, Beck DS, Smith AL (1986) Mouse hepatitis virus nasoencephalopathy is dependent upon virus strain and host genotype. Arch Virol 91: 247–256PubMedCrossRefGoogle Scholar
  2. 2.
    Boyle JF, Weismiller DG, Holmes KV (1987) Genetic resistance to mouse hepatitis virus correlates with the absence of virus-binding activity of target tissues. J Virol 61: 185–189PubMedGoogle Scholar
  3. 3.
    Chesebro B, Buller R, Portis J, Wehrly K (1990) Failure of human immunodeficiency virus entry and infection in CD4-positive human brain and skin cells. J Virol 64: 215–221PubMedGoogle Scholar
  4. 4.
    Clapham PR, Weber JN, Whitby D, Mcintosh K, Dalgleish AG, Maddon PJ, Deen KC, Sweet RW, Weiss RA (1989) Soluble CD4 blocks the infectivity of diverse strains of HIV and SIV for T cells and monocytes but not for brain and muscle cells. Nature 337: 368–370PubMedCrossRefGoogle Scholar
  5. 5.
    Collins AR, Knobler RL, Powell H, Buchmeier MJ (1982) Monoclonal antibodies to murine hepatitis virus-4 (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion. Virology 119: 358–371PubMedCrossRefGoogle Scholar
  6. 6.
    Dalziel RG, Lampert PW, Talbot PJ, Buchmeier MJ (1986) Site-specific alteration of murine hepatitis virus type 4 peplomer glycoprotein E2 results in reduced neurovirulence. J Virol 59: 463–471PubMedGoogle Scholar
  7. 7.
    De-Rossi A, Roncella S, Calabro ML, Ardrea E, Pasti M, Panozzo M, Mammano F, Ferrarini M, Chieco-Bianchi L (1990) Infection of Epstein-Barr virus-transformed lymphoblastoid B cells by the human immunodeficiency virus: evidence for a persistent and productive infection leading to B cell phenotypic changes. Eur J Immunol 20: 2041–2049PubMedCrossRefGoogle Scholar
  8. 8.
    Dveksler GS, Pensiero MN, Cardellichio CB, Williams RK, Jiang GS, Holmes KV, Dieffenbach CW (1991) Cloning of the mouse hepatitis virus (MHV) receptor: expression in human and hamster cell lines confers susceptibility to MHV. J Virol 65: 6881–6891PubMedGoogle Scholar
  9. 9.
    Ellis L, Clauser E, Morgan DO, Edery M, Roth RA, Rutter WJ (1986) Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose. Cell 45: 721–732PubMedCrossRefGoogle Scholar
  10. 10.
    Greve JM, Davis G, Meyer AM, Forte CP, Yost SC, Marlor CW, Kamarck ME, McClelland A (1989) The major human rhinovirus receptor is ICAM-1. Cell 56: 839–847PubMedCrossRefGoogle Scholar
  11. 11.
    Hirano N, Fujiwara K, Hino S, Matsumoto M (1974) Replication and plaque formation of mouse hepatitis virus (MHV-2) in mouse cell line DBT culture. Arch Ges Virusforsch 44: 298–302PubMedCrossRefGoogle Scholar
  12. 12.
    Knobler RL, Tunison LA, Oldstone MBA (1984) Host genetic control of mouse hepatitis virus type 4 (JHM strain) replication. I. Restriction of virus amplification and spread in macrophages from resistant mice. J Gen Virol 65: 1543–1548Google Scholar
  13. 13.
    Lindsley MD, Thiemann R, Rodrigues M (1991) Cytotoxic T cells isolated from the central nervous system of mice infected with Theiler’s virus. J Virol 65: 6612–6620PubMedGoogle Scholar
  14. 14.
    Maddon PJ, Dalgleish AG, McDougal JS, Clapham PR, Weiss RA, Axel R (1986) The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain. Cell 47: 333–348PubMedCrossRefGoogle Scholar
  15. 15.
    Mendelsohn CL, Wimmer E, Racaniello VR (1989) Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily. Cell 56: 855–865PubMedCrossRefGoogle Scholar
  16. 16.
    Robb JA, Bond CW (1979) Pathogenic murine coronaviruses. I. Characterization of biological behavior in vitro and virus-specific intracellular RNA of strongly neurotropic JHMV and weakly neurotropic A59 viruses. Virology 94: 352–370PubMedCrossRefGoogle Scholar
  17. 17.
    Stohlman SA, Freiinger JA (1978) Resistance to fatal central nervous system disease by mouse hepatitis virus, strain JHM. I. Genetic analysis. Immunogenetics 6: 277–281Google Scholar
  18. 18.
    Turbide C, Rojas M, Stanners CP, Beauchemin N (1991) A mouse carcinoembry- onic antigen gene family member is a calcium-dependent cell adhesion molecule. J Biol Chem 266: 309–315PubMedGoogle Scholar
  19. 19.
    Williams RK, Jiang GS, Holmes, KV (1991) Receptor for mouse hepatitis virus is a member of the carcinoembryonic antigen family of glycoproteins. Proc Natl Acad Sei USA 88: 5533–5536CrossRefGoogle Scholar
  20. 20.
    Williams RK, Jiang GS, Snyder SW, Frana MF, Holmes KV (1990) Purification of the 110-kilodalton glycoprotein receptor for mouse hepatitis virus (MHV)-A59 from mouse liver and identification of a nonfunctional, homologous protein in MHV-resistant SJL/J mice. J Virol 64: 3817–3823PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • K. Yokomori
    • 1
  • M. M. C. Lai
    • 1
  1. 1.Howard Hughes Medical Institute and Department of MicrobiologyUniversity of Southern California School of MedicineLos AngelesUSA

Personalised recommendations