Molecular characterization of Borna virus RNA

  • J. M. Pyper
  • L. Brown
  • J. E. Clements
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 9)


Borna disease virus is cell-associated in infected animals. Antibodies in animals are directed against BDV proteins of 38/39, 24, and 14.5 kD. cDNA clones that encode these proteins hybridize to five mRNAs of 10.5, 3.6, 2.1, 1.4, and 0.85 kb. The 10.5, 3.6, 2.1, and 0.85 kb RNAs are 3′ co-terminal; the 1.4 kb RNA is contained within the 10.5, 3.6, and 2.1 kb species but is not 3′ co-terminal. A negative strand 10 kb RNA is also present in infected cells. To determine which of the large 10 kb species represents the genomic RNA, strand-specific probes were used for Northern analyses of RNA from infectious particles isolated by Freon extraction of BDV-infected rat brain. RNA purified from these particles contained both positive and negative sense 10 kb species. Treatment of particles with RNaseA before isolation of RNA resulted in detection of only negative strand species, suggesting that BDV is a negative strand RNA virus. However, the genomic organization of BDV is unlike any known negative strand RNA virus.


Infected Animal Proteolytic Fragment Negative Strand Subtractive Library Guanidinium Isothiocyanate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Heinig H (1969) Die Borna’sche Krankheit der Pferde und Schafe. In: Roehrer H (ed) Handbuch der Virusinfektionen bei Tieren, vol 4. VEB Fischer, Jena, pp 83–148Google Scholar
  2. 2.
    Zwick W, Seifried O, Witte J (1927) Experimentelle Untersuchungen ueber die seuchenhafte Gehirn- und Rueckenmarks-entzuendung der Pferde ( Bornasche Krankheit ). Z Infektionskrkh 30: 42–136Google Scholar
  3. 3.
    Bode L, Riegel S, Ludwig H, Amsterdam J, Lange W, Koprowski H (1988) Borna disease virus specific antibodies in partients with HIV infection and with mental disorders. Lancet 2: 689PubMedCrossRefGoogle Scholar
  4. 4.
    Rott R, Herzog S, Fleischer B, Winokur A, Amsterdam J, Dyson W (1985) Detection of serum antibodies to Borna disease virus in patients with psychiatric disorders. Science 228: 755–756PubMedCrossRefGoogle Scholar
  5. 5.
    Carbone KM, Duchala CS, Griffin JW, Kincaid AL, Narayan O (1987) Pathogenesis of Borna disease in rats: evidence that intra-axonal spread is the major route for virus dissemination and the determinant for disease incubation. J Virol 61: 3431–3440PubMedGoogle Scholar
  6. 6.
    Morales JA, Herzog S, Kompter C, Frese K, Rott R (1988) Axonal transpot of Borna disease virus along olfactory pathways in spontaneously and experimentally infected rats. Med Microbiol Immunol 177: 51–68PubMedCrossRefGoogle Scholar
  7. 7.
    Shankar V, Kao M, Hamir AN, Sheng H, Koprowski H, Dietschold B (1992) Kinetics of virus spread and changes in levels of several cytokine mRNAs in the brain after intranasal infection of rats with Borna disease virus. J Virol 66: 992–998PubMedGoogle Scholar
  8. 8.
    Dittrich W, Bode L, Ludwig H, Kao M, Schneider K (1989) Learning deficiencies in Borna disease virus-infected but clinically healthy rats. Biol Psychol 26: 818–828CrossRefGoogle Scholar
  9. 9.
    Narayan O, Herzog S, Frese K, Scheefers H, Rott R (1983) Behavioral disease in rats caused by immunopathological responses to persistent Borna virus in the brain. Science 220: 1401–1403PubMedCrossRefGoogle Scholar
  10. 10.
    Narayan O, Herzog S, Frese K, Scheefers H, Rott R (1983) Pathogenesis of Borna disease in rats: immune-mediated ophthalmoencephalopathy causing blindness and behavioral abnormalities. J Infect Dis 148: 305–315PubMedCrossRefGoogle Scholar
  11. 11.
    Stitz L, Soeder D, Deschl U, Frese K, Rott R (1989) Inhibition of immunemediated meningoencephalitis in persistently Borna virus-infected rats by cyclosporine A. J Immunol 143: 4250–4256PubMedGoogle Scholar
  12. 12.
    Herzog S, Wonigeit K, Frese K, Hedrich HJ, Rott R (1985) Effect of Borna disease virus infection on athymic rats. J Gen Virol 66: 503–508PubMedCrossRefGoogle Scholar
  13. 13.
    Rieht JA, Stitz L, Wekerle H, Rott R (1989) Borna disease, a progressive menin- goencephalomyelitis as a model for CD4+ T cell-mediated immunopathology in the brain. J Exp Med 170: 1045–1050CrossRefGoogle Scholar
  14. 14.
    Carbone KM, Trapp BD, Griffin JW, Duchala CS, Narayan O (1989) Astrocytes and Schwann cells are virus-host cells in the nervous system of rats with Borna disease. J Neuropathol Exp Neurol 48: 631–644PubMedCrossRefGoogle Scholar
  15. 15.
    Buchmeier MJ, Welsh RM, Dutko FJ, Oldstone MBA (1980) The virology and immunobiology of LCM virus infection. Adv Immunol 30: 275–331PubMedCrossRefGoogle Scholar
  16. 16.
    Ahmed R (1988) Curing of a congenitally acquired chronic virus infection: Acquisition of T-cell competence by a previously “tolerant” host. In: Lopez C (ed) Immunobiology and pathogenesis of persistent virus infections. ASV Publ., Washington, pp 159–167Google Scholar
  17. 17.
    Elford WG, Galloway IA (1935) Filtration of the virus of Borna disease through graded collodion membranes. Br J Exp Pathol 14: 196–205Google Scholar
  18. 18.
    Danner K, Mayr A (1979) In vitro studies on Borna virus. II. Properties of the virus. Arch Virol 61: 261–271Google Scholar
  19. 19.
    Pauli G, Ludwig H (1985) Increase of virus yields and releases of Borna disease virus from persistently infected cells. Virus Res 2: 29–33PubMedCrossRefGoogle Scholar
  20. 20.
    Rieht JA, Clements JE, Herzog S, Pyper J, Wahn K, Becht H (1993) Analysis of virus-specific RNA species in Freon-113 preparations of the Borna disease virus. Med Microbiol Immunol (in press)Google Scholar
  21. 21.
    Haas B, Becht H, Rott R (1986) Purification and properties of an intranuclear virus-specific antigen from tissues infected with Borna disease virus. J Gen Virol 67: 235–241PubMedCrossRefGoogle Scholar
  22. 22.
    Schaedler R, Diringer H, Ludwig H (1985) Isolation and characterization of a 14500 molecular weight protein from brains and tissue cultures persistently infected with Borna disease virus. J Gen Virol 66: 2479–2484CrossRefGoogle Scholar
  23. 23.
    Thierer J, Riehle H, Grebenstein O, Binz T, Herzog S, Thiedemann N, Stitz L, Rott R, Lottspeich F, Niemann H (1992) The 24 K protein of Borna disease virus. J Gen Virol 73: 413–416PubMedCrossRefGoogle Scholar
  24. 24.
    VandeWoude S, Richt JA, Zink MC, Rott R, Narayan O, Clements JE (1990) A Borna virus cDNA encoding a protein recognized by antibodies in humans with behavioral diseases. Science 250: 1278–1281CrossRefGoogle Scholar
  25. 25.
    Richt JA, VandeWoude S, Zink MC, Narayan O, Clements JE (1991) Analysis of Borna disease virus-specific RNAs in infected cells and tissues. J Gen Virol 72: 2251–2255PubMedCrossRefGoogle Scholar
  26. 26.
    delaTorre JC, Carbone KM, Lipkin WI (1990) Molecular characterization of the Borna disease agent. Virology 179: 853–856CrossRefGoogle Scholar
  27. 27.
    Lipkin WI, Travis GH, Carbone KM, Wilson MC (1990) Isolation and characterization of Borna disease agent cDNA clones. Proc Natl Acad Sci USA 87: 4184–4188PubMedCrossRefGoogle Scholar
  28. 28.
    Pyper JM, Richt JA, Brown L, Rott R, Narayan O, Clements JE (1993) Genomic organization of the structural proteins of Borna disease virus revealed by a cDNA clone encoding the 38 kD protein. Virology 195: 229–238PubMedCrossRefGoogle Scholar
  29. 29.
    Frohman MA, Dush MK, Martin GR (1988) Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85: 8998–9002Google Scholar
  30. 30.
    Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • J. M. Pyper
    • 1
  • L. Brown
    • 1
  • J. E. Clements
    • 1
  1. 1.Division of Comparative MedicineThe Johns Hopkins University School of MedicineBaltimoreUSA

Personalised recommendations