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Replication and translation of cowpea mosaic virus RNAs are tightly linked

  • Conference paper
Positive-Strand RNA Viruses

Part of the book series: Archives of Virology Supplementum ((ARCHIVES SUPPL,volume 9))

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Summary

The genome of cowpea mosaic virus (CPMV) is divided among two positive strand RNA molecules. B-RNA is able to replicate independently from M-RNA in cowpea protoplasts. Replication of mutant B-transcripts could not be supported by co-inoculated wild-type B-RNA, indicating that B-RNA cannot be efficiently replicated in trans. Hence replication of a B-RNA molecule is tightly linked to its translation and/or at least one of the replicative proteins functions in cis only. Remarkably also for efficient replication of M-RNA one of its translation products was found to be required in cis. This 58K protein possibly helps in directing the B-RNA-encoded replication complex to the M-RNA. In order to identify the viral polymerase the CPMV B-RNA-specific proteins have been produced individually in cowpea protoplasts using CaMV 35S promoter based expression vectors. Only protoplasts trans- fected with a vector containing the 200K coding sequence were able to support replication of co-transfected M-RNA. Despite this, CPMV- specific RNA polymerase activity could not be detected in extracts of these protoplasts using a poly(A)/oligo(U) assay. These results indicate that, in contrast to the poliovirus polymerase, the CPMV polymerase is not able to accept oligo(U) as a primer and in addition support the concept that translation and replication are linked.

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Author information

Author notes

  1. H. van Bokhoven

    Present address: Department of Human Genetics, University Hospital, University of Nijmegen, Nijmegen, The Netherlands

  2. O. Le Gall

    Present address: Station de Pathologie Végétale, INRA, Villenave D’Ornon Cédex, France

Authors and Affiliations

  1. Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands

    Dr. J. Wellink, H. van Bokhoven, O. Le Gall, J. Verver & A. van Kammen

Authors
  1. Dr. J. Wellink
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  2. H. van Bokhoven
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  3. O. Le Gall
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  4. J. Verver
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  5. A. van Kammen
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Editor information

Editors and Affiliations

  1. Department of Biology, Georgia State University, Atlanta, Georgia, USA

    Margo A. Brinton

  2. Arthropod-borne and Infectious Disease Laboratory (AIDL), Colorado State University, Fort Collins, Colorado, USA

    Charles H. Calisher

  3. Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin, USA

    Roland Rueckert

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© 1994 Springer-Verlag

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Wellink, J., van Bokhoven, H., Le Gall, O., Verver, J., van Kammen, A. (1994). Replication and translation of cowpea mosaic virus RNAs are tightly linked. In: Brinton, M.A., Calisher, C.H., Rueckert, R. (eds) Positive-Strand RNA Viruses. Archives of Virology Supplementum, vol 9. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9326-6_38

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  • DOI: https://doi.org/10.1007/978-3-7091-9326-6_38

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82522-8

  • Online ISBN: 978-3-7091-9326-6

  • eBook Packages: Springer Book Archive

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