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Identification and characterization of host factor interactions with cis-acting elements of rubella virus RNA

  • Conference paper
Positive-Strand RNA Viruses

Part of the book series: Archives of Virology Supplementum ((ARCHIVES SUPPL,volume 9))

Summary

We have analyzed the function of cis-acting elements of rubella virus RNA and the components which interact with these elements in viral RNA replication. We demonstrated that the 5′- and 3′-terminal sequences from RV RNA promote translation and negative- strand RNA synthesis of chimeric chloramphenicol acetyltransferase (CAT) RNAs. These sequences have a potential to form stem-loop (SL) structures and bind cellular proteins specifically in RNA gel-shift and UV cross-linking assays. The 5’ end binding proteins were identified to be Ro/SSA-associated antigens by virtue of being recognized in an RNA complex by an autoimmune patient serum with Ro antigen type specificity. Purification and sequence analysis of the 3’ end binding protein revealed that it is a homologue of human calreticulin. The role of host proteins in RV replication is discussed.

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Author information

Author notes

  1. X.-Q. Cao

    Present address: Laboratory of Pulmonary and Molecular Immunology, NHLBI, NIH, Bethesda, Maryland, USA

Authors and Affiliations

  1. Division of Hematologic Products, CBER, Food and Drug Administration, National Institutes of Health, Bethesda, Maryland, USA

    Dr. H. L. Nakhasi, N. K. Singh, G. P. Pogue & X.-Q. Cao

  2. Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA

    T. A. Rouault

Authors
  1. Dr. H. L. Nakhasi
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  2. N. K. Singh
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  3. G. P. Pogue
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  4. X.-Q. Cao
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  5. T. A. Rouault
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Editor information

Editors and Affiliations

  1. Department of Biology, Georgia State University, Atlanta, Georgia, USA

    Margo A. Brinton

  2. Arthropod-borne and Infectious Disease Laboratory (AIDL), Colorado State University, Fort Collins, Colorado, USA

    Charles H. Calisher

  3. Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin, USA

    Roland Rueckert

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© 1994 Springer-Verlag

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Nakhasi, H.L., Singh, N.K., Pogue, G.P., Cao, XQ., Rouault, T.A. (1994). Identification and characterization of host factor interactions with cis-acting elements of rubella virus RNA. In: Brinton, M.A., Calisher, C.H., Rueckert, R. (eds) Positive-Strand RNA Viruses. Archives of Virology Supplementum, vol 9. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9326-6_26

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  • DOI: https://doi.org/10.1007/978-3-7091-9326-6_26

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82522-8

  • Online ISBN: 978-3-7091-9326-6

  • eBook Packages: Springer Book Archive

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