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Sequences at the ends of RNA-2 of I6, a recombinant tobravirus

  • D. J. Robinson
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 9)

Summary

Tobravirus isolate I6 is an anomalous strain of tobacco rattle virus (TRV), which has biological properties typical of TRV, but which is serologically related to pea early-browning virus (PEBV). Its RNA-2 species is a natural recombinant molecule which contains internal sequences, including the particle protein gene, resembling those of PEBV, but with some TRV-like sequences at each end. Sequencing of RNA-2 of isolate I6 showed that, at the 3′ end, it is almost identical to RNA-2 of PEBV strain SP5, and TRV-like sequences are limited to the 25 residues that are common to both viruses. However, unlike PEBV RNA-2, I6 RNA-2 can be replicated by TRV RNA-l-coded enzymes. Thus, specific recognition of RNA-2 by TRV replicase seems not to involve sequences at the 3′ end. About 275 residues at the 5′ end of I6 RNA-2 are similar to those in RNA-2 of TRV strain TCM, and are joined to the PEBV-SP5-like subgenomic promoter and coding sequences by about 100 residues of uncertain origin. These results support the idea that I6 RNA-2 originated by recombination between TRV and PEBV RNA-2 molecules, although the exact position of the junction and therefore the mechanism involved are unclear.

Keywords

Tobacco Rattle Virus Barley Stripe Mosaic Virus Brome Mosaic Virus Subgenomic Promoter Tobacco Rattle Virus Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • D. J. Robinson
    • 1
  1. 1.Scottish Crop Research InstituteInvergowrie, DundeeUK

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