Sequences at the ends of RNA-2 of I6, a recombinant tobravirus

  • D. J. Robinson
Conference paper
Part of the Archives of Virology Supplementum book series (ARCHIVES SUPPL, volume 9)


Tobravirus isolate I6 is an anomalous strain of tobacco rattle virus (TRV), which has biological properties typical of TRV, but which is serologically related to pea early-browning virus (PEBV). Its RNA-2 species is a natural recombinant molecule which contains internal sequences, including the particle protein gene, resembling those of PEBV, but with some TRV-like sequences at each end. Sequencing of RNA-2 of isolate I6 showed that, at the 3′ end, it is almost identical to RNA-2 of PEBV strain SP5, and TRV-like sequences are limited to the 25 residues that are common to both viruses. However, unlike PEBV RNA-2, I6 RNA-2 can be replicated by TRV RNA-l-coded enzymes. Thus, specific recognition of RNA-2 by TRV replicase seems not to involve sequences at the 3′ end. About 275 residues at the 5′ end of I6 RNA-2 are similar to those in RNA-2 of TRV strain TCM, and are joined to the PEBV-SP5-like subgenomic promoter and coding sequences by about 100 residues of uncertain origin. These results support the idea that I6 RNA-2 originated by recombination between TRV and PEBV RNA-2 molecules, although the exact position of the junction and therefore the mechanism involved are unclear.


Tobacco Rattle Virus Barley Stripe Mosaic Virus Brome Mosaic Virus Subgenomic Promoter Tobacco Rattle Virus Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Angenent GC, Linthorst HJM, Van Belkum AF, Cornelissen BJC, Bol JF (1986) RNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene. Nucleic Acids Res 14: 4673–4682PubMedCrossRefGoogle Scholar
  2. 2.
    Angenent GC, Posthumus E, Brederode FT, Bol JF (1989) Genome structure of tobacco rattle virus strain PLB: further evidence on the occurrence of RNA recombination among tobraviruses. Virology 171: 271–274PubMedCrossRefGoogle Scholar
  3. 3.
    Bujarski JJ, Dzianott AM (1991) Generation and analysis of nonhomologous RNA-RNA recombinants in brome mosaic virus: sequence complementarities at crossover sites. J Virol 65: 4153–4159PubMedGoogle Scholar
  4. 4.
    Cornelissen BJC, Linthorst HJM, Brederode FT, Bol JF (1986) Analysis of the genome structure of tobacco rattle virus strain PSG. Nucleic Acids Res 14: 2157–2169PubMedCrossRefGoogle Scholar
  5. 5.
    Edwards MC, Petty ITD, Jackson AO (1992) RNA recombination in the genome of barley stripe mosaic virus. Virology 189: 389–392PubMedCrossRefGoogle Scholar
  6. 6.
    Goulden MG, Lomonossoff GP, Davies JW, Wood KR (1990) The complete nucleotide sequence of PEBV RNA2 reveals the presence of a novel open reading frame and provides insights into the structure of tobraviral subgenomic promoters. Nucleic Acids Res 18: 4507–4512PubMedCrossRefGoogle Scholar
  7. 7.
    Goulden MG, Lomonossoff GP, Wood KR, Davies JW (1991) A model for the generation of tobacco rattle virus (TRV) anomalous isolates: pea early browning virus RNA-2 acquires TRV sequences from both RNA-1 and RNA-2. J Gen Virol 72: 1751–1754PubMedCrossRefGoogle Scholar
  8. 8.
    Hamilton WDO, Boccara M, Robinson DJ, Baulcombe DC (1987) The complete nucleotide sequence of tobacco rattle virus RNA-1. J Gen Virol 68: 2563–2575PubMedCrossRefGoogle Scholar
  9. 9.
    Harrison BD, Robinson DJ (1978) The tobraviruses. Adv Virus Res 23: 25–77PubMedCrossRefGoogle Scholar
  10. 10.
    Kirkegaard K, Baltimore D (1986) The mechanism of RNA recombination in polio virus. Cell 47: 433–443PubMedCrossRefGoogle Scholar
  11. 11.
    Lister RM (1968) Functional relationships between virus-specific products of infection by viruses of the tobacco rattle type. J Gen Virol 2: 43–58CrossRefGoogle Scholar
  12. 12.
    MacFarlane SA, Taylor SC, King DI, Hughes G, Davies JW (1989) Pea early browning virus RNA1 encodes four polypeptides including a putative zinc-finger protein. Nucleic Acids Res 17: 2245–2260PubMedCrossRefGoogle Scholar
  13. 13.
    Robinson DJ, Harrison BD (1985) Unequal variation in the two genome parts of tobraviruses and evidence for the existence of three separate viruses. J Gen Virol 66: 171–176CrossRefGoogle Scholar
  14. 14.
    Robinson DJ, Harrison BD (1985) Evidence that broad bean yellow band virus is a new serotype of pea early-browning virus. J Gen Virol 66: 2003–2009CrossRefGoogle Scholar
  15. 15.
    Robinson DJ, Hamilton WDO, Harrison BD, Baulcombe DC (1987) Two anomalous tobravirus isolates: evidence for RNA recombination in nature. J Gen Virol 68: 2551–2561CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • D. J. Robinson
    • 1
  1. 1.Scottish Crop Research InstituteInvergowrie, DundeeUK

Personalised recommendations