Summary
Marmosets injected intrahepatically with nucleic acids (cDNA and RNA transcripts) representing the full-length genome of the wild-type HM-175 strain of hepatitis A virus experienced acute hepatitis and seroconversion to hepatitis A virus capsid proteins. The hepatitis was comparable in severity to that caused by infection with the wild-type virus. The viral cDNA and the hepatitis A virus recovered from the feces of an injected animal contained the same marker mutation. Therefore, intermediate cell culture steps can be omitted and the virulence of a hepatitis A virus encoded by a cDNA clone can be evaluated by direct transfection of marmosets.
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© 1994 Springer-Verlag
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Emerson, S.U., Lewis, M., Govindarajan, S., Shapiro, M., Moskal, T., Purcell, R.H. (1994). In vivo transfection by hepatitis A virus synthetic RNA. In: Brinton, M.A., Calisher, C.H., Rueckert, R. (eds) Positive-Strand RNA Viruses. Archives of Virology Supplementum, vol 9. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9326-6_20
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DOI: https://doi.org/10.1007/978-3-7091-9326-6_20
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-82522-8
Online ISBN: 978-3-7091-9326-6
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