In vivo transfection by hepatitis A virus synthetic RNA
Marmosets injected intrahepatically with nucleic acids (cDNA and RNA transcripts) representing the full-length genome of the wild-type HM-175 strain of hepatitis A virus experienced acute hepatitis and seroconversion to hepatitis A virus capsid proteins. The hepatitis was comparable in severity to that caused by infection with the wild-type virus. The viral cDNA and the hepatitis A virus recovered from the feces of an injected animal contained the same marker mutation. Therefore, intermediate cell culture steps can be omitted and the virulence of a hepatitis A virus encoded by a cDNA clone can be evaluated by direct transfection of marmosets.
KeywordsVirulent Virus African Green Monkey Kidney Chimeric Virus African Green Monkey Kidney Cell Rabbit Hemorrhagic Disease
Unable to display preview. Download preview PDF.
- 8.Emerson SU (1993) Unpubl. dataGoogle Scholar